##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AfaI AflIII AgeI AhdI Alw44I
AocI ApaLI ApoI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
BalI BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BglII BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DdeI
DraI DraIII DrdI Eam1105I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MaeII MaeIII MamI
MfeI MluI MluNI MscI MunI
NaeI NcoI NdeI NgoMIV NheI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tsp45I Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccIII 570 TCCGGA T^CCGGA
AflII 409 CTTAAG C^TTAAG
AlwNI 352 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 405 AGCGCT AGC^GCT
AvrII 531 CCTAGG C^CTAGG
BfrI 409 CTTAAG C^TTAAG
BlnI 531 CCTAGG C^CTAGG
BsaWI 570 TCCGGA [AT]^CCGG[AT]
BseAI 570 TCCGGA T^CCGGA
BsgI 118 GTGCAG GTGCAG(16/14)
BsiEI 126 CGGCCG CG[GA][CT]^CG
BsmFI 304 GGGAC GGGAC(10/14)
BspEI 570 TCCGGA T^CCGGA
BsrFI 200 ACCGGC [GA]^CCGG[CT]
BstF5I 314 CATCC GGATG(2/0)
BstYI 176 AGATCC [GA]^GATC[CT]
Cfr10I 200 ACCGGC [GA]^CCGG[CT]
DsaI 450 CCGCGG C^C[GA][CT]GG
EagI 126 CGGCCG C^GGCCG
EclXI 126 CGGCCG C^GGCCG
Eco47III 405 AGCGCT AGC^GCT
Eco52I 126 CGGCCG C^GGCCG
EcoNI 132 CCTCATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 531 CCTAGG C^C[AT][AT]GG
FokI 314 CATCC GGATG(9/13)
HgaI 204 GCGTC GACGC(5/10)
HphI 144 GGTGA GGTGA(8/7)
KspI 450 CCGCGG CCGC^GG
MflI 176 AGATCC [GA]^GATC[CT]
MroI 570 TCCGGA T^CCGGA
MseI 410 TTAA T^TAA
MslI 284 CACGCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII 135 CATG CATG^
NotI 125 GCGGCCGC GC^GGCCGC
PleI 253 GACTC GAGTC(4/5)
PvuII 154 CAGCTG CAG^CTG
SacII 450 CCGCGG CCGC^GG
SfaNI 313 GCATC GCATC(5/9)
SmaI 317 CCCGGG CCC^GGG
StyI 531 CCTAGG C^C[AT][AT]GG
TfiI 147 GAATC G^A[AT]TC
Tru9I 410 TTAA T^TAA
XhoII 176 AGATCC [GA]^GATC[CT]
XmaCI 317 CCCGGG C^CCGGG
XmaI 317 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
ApaI 444 GGGCCC GGGCC^C
520 GGGCCC
AscI 99 GGCGCGCC GG^CGCGCC
275 GGCGCGCC
AspHI 184 GAGCTC G[AT]GC[AT]^C
415 GAGCTC
AvaI 317 CCCGGG C^[CT]CG[GA]G
496 CCCGAG
AvaII 305 GGACC G^G[AT]CC
333 GGACC
BanI 77 GGCGCC G^G[CT][GA]CC
466 GGCGCC
BbeI 77 GGCGCC GGCGC^C
466 GGCGCC
BsiHKAI 184 GAGCTC G[AT]GC[AT]^C
415 GAGCTC
BsoBI 317 CCCGGG C^[CT]CG[GA]G
496 CCCGAG
BssHII 100 GCGCGC G^CGCGC
276 GCGCGC
EaeI 126 CGGCCG [CT]^GGCC[GA]
359 TGGCCG
EarI 298 CTCTTC CTCTTC(1/4)
64 GAAGAG
HinfI 147 GAATC G^A[ACGT]TC
253 GACTC
KasI 77 GGCGCC G^GCGCC
466 GGCGCC
Ksp632I 298 CTCTTC CTCTTC(1/4)
64 GAAGAG
NarI 77 GGCGCC GG^CGCC
466 GGCGCC
PpuMI 304 GGGACCC [GA]G^G[AT]CC[CT]
332 AGGACCT
PstI 157 CTGCAG CTGCA^G
208 CTGCAG
SacI 184 GAGCTC GAGCT^C
415 GAGCTC
SapI 64 GAAGAGC GCTCTTC(1/4)
297 GCTCTTC
SfcI 157 CTGCAG C^T[GA][CT]AG
208 CTGCAG
SfoI 77 GGCGCC GGC^GCC
466 GGCGCC
SmlI 187 CTCAAG C^T[CT][GA]AG
409 CTTAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
BanII BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsiYI BslI Bsp1286I BsrI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DpnII DraII EcoO109I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII ItaI MaeI
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIV Sau3AI Sau96I ScrFI TseI
TspRI