Restriction map for
pF1KB9784
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AviII AvrII BamHI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BseRI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BsrI BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FspI HincII HindII
HindIII HinfI HpaI HphI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MroI MseI MslI MunI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PleI PmeI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I TspRI Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AfaI 531 GTAC GT^AC
AlwI 543 GATCC GGATC(4/5)
AlwNI 286 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 396 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 356 TGGCCA TGG^CCA
BbeI 26 GGCGCC GGCGC^C
BbrPI 310 CACGTG CAC^GTG
BbsI 3 GAAGAC GAAGAC(2/6)
BpuAI 3 GAAGAC GAAGAC(2/6)
BsaAI 310 CACGTG [CT]AC^GT[GA]
BsgI 40 GTGCAG GTGCAG(16/14)
BsiEI 130 CGGCCG CG[GA][CT]^CG
BsmAI 343 GAGAC GTCTC(1/5)
BsmBI 343 GAGACG CGTCTC(1/5)
BsrBI 250 GAGCGG CCGCTC(-3/-3)
BssHII 315 GCGCGC G^CGCGC
BstAPI 255 GCAGCGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 542 AGATCC [GA]^GATC[CT]
EagI 130 CGGCCG C^GGCCG
EclXI 130 CGGCCG C^GGCCG
Eco52I 130 CGGCCG C^GGCCG
EcoT14I 113 CCAAGG C^C[AT][AT]GG
FseI 131 GGCCGGCC GGCCGG^CC
HaeII 26 GGCGCC [GA]GCGC^[CT]
KasI 26 GGCGCC G^GCGCC
MaeII 311 ACGT A^CGT
MaeIII 209 GTGAC ^GT[ACGT]AC
MboII 3 GAAGA GAAGA(8/7)
MflI 542 AGATCC [GA]^GATC[CT]
MluNI 356 TGGCCA TGG^CCA
MscI 356 TGGCCA TGG^CCA
NarI 26 GGCGCC GG^CGCC
NlaIII 399 CATG CATG^
PflFI 396 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 310 CACGTG CAC^GTG
PmlI 310 CACGTG CAC^GTG
PstI 523 CTGCAG CTGCA^G
RsaI 531 GTAC GT^AC
SfcI 523 CTGCAG C^T[GA][CT]AG
SfiI 495 GGCCACGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 26 GGCGCC GGC^GCC
StuI 420 AGGCCT AGG^CCT
StyI 113 CCAAGG C^C[AT][AT]GG
TaqI 341 TCGA T^CGA
Tsp45I 209 GTGAC ^GT[GC]AC
Tth111I 396 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 341 TCGA T^CGA
XhoII 542 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 316 CGCG CG^CG
318 CGCG
AcyI 26 GGCGCC G[GA]^CG[CT]C
72 GACGCC
AvaII 83 GGTCC G^G[AT]CC
184 GGACC
BanI 26 GGCGCC G^G[CT][GA]CC
562 GGCACC
BpmI 232 CTGGAG CTGGAG(16/14)
424 CTGGAG
BsaHI 26 GGCGCC G[GA]^CG[CT]C
72 GACGCC
BsmFI 70 GGGAC GGGAC(10/14)
84 GTCCC
BspMI 521 ACCTGC ACCTGC(4/8)
471 GCAGGT
BstUI 316 CGCG CG^CG
318 CGCG
DpnII 339 GATC ^GATC
543 GATC
DsaI 22 CCACGG C^C[GA][CT]GG
497 CCACGG
HgaI 72 GACGC GACGC(5/10)
345 GACGC
Hin1I 26 GGCGCC G[GA]^CG[CT]C
72 GACGCC
MboI 339 GATC ^GATC
543 GATC
MspA1I 223 CAGCGG C[AC]G^C[GT]G
256 CAGCGG
MvnI 316 CGCG CG^CG
318 CGCG
NdeII 339 GATC ^GATC
543 GATC
PpuMI 82 GGGTCCC [GA]G^G[AT]CC[CT]
183 AGGACCC
Sau3AI 339 GATC ^GATC
543 GATC
SfaNI 261 GATGC GCATC(5/9)
218 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI ApaI AvaI BanII
BbvI BcnI BglI BmyI BsaJI
BsiYI BslI BsoBI Bsp1286I BsrFI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DraII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MnlI
MspI MvaI MwoI NaeI NciI
NgoMIV NlaIV Sau96I ScrFI SmaI
TseI XmaCI XmaI