Restriction map for
pF1KB9782
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvrII BalI BamHI
BbrPI BbvCI BciVI BclI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaI BsaWI BseAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstF5I BstPI BstXI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcyI 383 GGCGCC G[GA]^CG[CT]C
Alw44I 298 GTGCAC G^TGCAC
ApaLI 298 GTGCAC G^TGCAC
AvaII 324 GGACC G^G[AT]CC
BbeI 383 GGCGCC GGCGC^C
BbsI 165 GTCTTC GAAGAC(2/6)
BcgI 360 GCAGGAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 330 CCGGG CC^[GC]GG
BfaI 181 CTAG C^TAG
BlpI 273 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 273 GCTGAGC GC^T[ACGT]AGC
BpuAI 165 GTCTTC GAAGAC(2/6)
BsaHI 383 GGCGCC G[GA]^CG[CT]C
BseRI 316 GAGGAG GAGGAG(10/8)
BsgI 403 GTGCAG GTGCAG(16/14)
BsmAI 448 GTCTC GTCTC(1/5)
BstAPI 231 GCAACTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 273 GCTGAGC GC^T[ACGT]AGC
DsaI 452 CCACGG C^C[GA][CT]GG
EcoNI 24 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 293 CCAAGG C^C[AT][AT]GG
FseI 132 GGCCGGCC GGCCGG^CC
HaeII 383 GGCGCC [GA]GCGC^[CT]
HgaI 535 GACGC GACGC(5/10)
Hin1I 383 GGCGCC G[GA]^CG[CT]C
HinfI 460 GACTC G^A[ACGT]TC
HphI 505 GGTGA GGTGA(8/7)
KasI 383 GGCGCC G^GCGCC
MaeI 181 CTAG C^TAG
MaeIII 533 GTGAC ^GT[ACGT]AC
MboII 166 TCTTC GAAGA(8/7)
NarI 383 GGCGCC GG^CGCC
NciI 330 CCGGG CC^[GC]GG
NlaIII 489 CATG CATG^
PleI 460 GACTC GAGTC(4/5)
SfoI 383 GGCGCC GGC^GCC
SgrAI 567 CACCGGCG C[GA]^CCGG[CT]G
StyI 293 CCAAGG C^C[AT][AT]GG
Tsp45I 533 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 418 CGCG CG^CG
537 CGCG
AfaI 42 GTAC GT^AC
213 GTAC
AlwI 21 GATCC GGATC(4/5)
57 GGATC
ApaI 246 GGGCCC GGGCC^C
589 GGGCCC
AspHI 70 GAGCAC G[AT]GC[AT]^C
298 GTGCAC
AviII 143 TGCGCA TGC^GCA
422 TGCGCA
BsiEI 35 CGGCCG CG[GA][CT]^CG
131 CGGCCG
BsiHKAI 70 GAGCAC G[AT]GC[AT]^C
298 GTGCAC
BsrI 234 ACTGG ACTGG(1/-1)
312 ACTGG
BstUI 418 CGCG CG^CG
537 CGCG
DpnII 21 GATC ^GATC
58 GATC
EaeI 35 CGGCCG [CT]^GGCC[GA]
131 CGGCCG
EagI 35 CGGCCG C^GGCCG
131 CGGCCG
EclXI 35 CGGCCG C^GGCCG
131 CGGCCG
Eco52I 35 CGGCCG C^GGCCG
131 CGGCCG
FspI 143 TGCGCA TGC^GCA
422 TGCGCA
MboI 21 GATC ^GATC
58 GATC
MspA1I 352 CAGCTG C[AC]G^C[GT]G
394 CAGCTG
MvnI 418 CGCG CG^CG
537 CGCG
NaeI 133 GCCGGC GCC^GGC
599 GCCGGC
NdeII 21 GATC ^GATC
58 GATC
NgoMIV 133 GCCGGC G^CCGGC
599 GCCGGC
PstI 355 CTGCAG CTGCA^G
556 CTGCAG
PvuII 352 CAGCTG CAG^CTG
394 CAGCTG
RsaI 42 GTAC GT^AC
213 GTAC
Sau3AI 21 GATC ^GATC
58 GATC
SfaNI 341 GCATC GCATC(5/9)
366 GCATC
SmlI 217 CTTGAG C^T[CT][GA]AG
226 CTCAAG
TaqI 60 TCGA T^CGA
98 TCGA
TthHB8I 60 TCGA T^CGA
98 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI BanI BanII BbvI
BmyI BpmI BsaJI BsiYI BslI
Bsp1286I BsrFI BssKI BstNI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
EcoO109I EcoRII FauI Fnu4HI HaeIII
HapII HhaI HinP1I HpaII ItaI
MnlI MspI MvaI MwoI NlaIV
Sau96I ScrFI SfcI TseI TspRI