##################################
# NO CUT ENZYMES (  218 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BbvI        BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       Cfr10I      ClaI        CpoI        
DdeI        DpnII       DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FokI        
FseI        FspI        HaeII       HincII      HindII      
HindIII     HpaI        HphI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MaeIII      
MamI        MboI        MboII       MfeI        MflI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MunI        MvnI        MwoI        NaeI        
NarI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AciI           91  GCGG                 CCGC(-3/-1)
AcyI           93  GGCGTC               G[GA]^CG[CT]C
BsaHI          93  GGCGTC               G[GA]^CG[CT]C
BsaI           81  GGTCTC               GGTCTC(1/5)
BsmAI          82  GTCTC                GTCTC(1/5)
BsmFI         154  GTCCC                GGGAC(10/14)
BspHI         104  TCATGA               T^CATGA
BstNI          24  CCAGG                CC^[AT]GG
CfoI           72  GCGC                 GCG^C
Cfr13I         27  GGCCC                G^G[ACGT]CC
EcoRII         24  CCAGG                ^CC[AT]GG
Fnu4HI         91  GCGGC                GC^[ACGT]GC
HaeIII         27  GGCC                 GG^CC
HgaI           94  GCGTC                GACGC(5/10)
HhaI           72  GCGC                 GCG^C
Hin1I          93  GGCGTC               G[GA]^CG[CT]C
HinP1I         72  GCGC                 G^CGC
HinfI         152  GAGTC                G^A[ACGT]TC
ItaI           91  GCGGC                GC^[ACGT]GC
MseI          176  TTAA                 T^TAA
MvaI           24  CCAGG                CC^[AT]GG
NspI          123  ACATGC               [GA]CATG^[CT]
PleI          152  GAGTC                GAGTC(4/5)
RcaI          104  TCATGA               T^CATGA
Sau96I         27  GGCCC                G^G[ACGT]CC
SfaNI          74  GCATC                GCATC(5/9)
Tru9I         176  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (    3 enzymes) #
###################################

BsaJI          23  CCCAGG               C^C[ACGT][ACGT]GG
              161  CCTCGG              
MnlI          102  CCTC                 CCTC(7/6)
              161  CCTC                
NlaIII        105  CATG                 CATG^
              124  CATG                


#########################################
# 3 OR MORE CUT ENZYMES (   10 enzymes) #
#########################################

AluI        BcnI        BsiYI       BslI        BssKI       
HapII       HpaII       MspI        NciI        ScrFI