Restriction map for
pF1KB9775
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# NO CUT ENZYMES ( 117 enzymes) #
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Acc65I AccIII AclI AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvrII BamHI BbeI
BbrPI BcgI BciVI BglI BlnI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspEI BspLU11I BsrDI BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CpoI DraI DraIII EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRV
EcoT22I FseI HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AatII 292 GACGTC GACGT^C
AccI 274 GTCTAC GT^[AC][GT]AC
AflII 789 CTTAAG C^TTAAG
Asp700I 204 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1295 TGGCCA TGG^CCA
BanII 1417 GAGCCC G[GA]GC[CT]^C
BbsI 1894 GTCTTC GAAGAC(2/6)
BbvCI 1812 CCTCAGC CCTCAGC(-5/-2)
BclI 725 TGATCA T^GATCA
BfaI 895 CTAG C^TAG
BfrI 789 CTTAAG C^TTAAG
BglII 1001 AGATCT A^GATCT
BpuAI 1894 GTCTTC GAAGAC(2/6)
BsaI 784 GAGACC GGTCTC(1/5)
BspDI 18 ATCGAT AT^CGAT
BspHI 728 TCATGA T^CATGA
BsrFI 98 ACCGGC [GA]^CCGG[CT]
BssSI 1993 CACGAG CACGAG(-5/-1)
BstAPI 348 GCAGCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 98 ACCGGC [GA]^CCGG[CT]
ClaI 18 ATCGAT AT^CGAT
DrdI 576 GACACAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1121 CCATGG C^C[GA][CT]GG
EcoRI 987 GAATTC G^AATTC
FbaI 725 TGATCA T^GATCA
MaeI 895 CTAG C^TAG
MluNI 1295 TGGCCA TGG^CCA
MscI 1295 TGGCCA TGG^CCA
NcoI 1121 CCATGG C^CATGG
PpuMI 1340 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 728 TCATGA T^CATGA
SspI 29 AATATT AAT^ATT
TfiI 1495 GATTC G^A[AT]TC
Tsp45I 2021 GTGAC ^GT[GC]AC
XmnI 204 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcsI 415 GAATTT [GA]^AATT[CT]
987 GAATTC
AcyI 292 GACGTC G[GA]^CG[CT]C
1823 GGCGTC
Aor51HI 38 AGCGCT AGC^GCT
1529 AGCGCT
ApoI 415 GAATTT [GA]^AATT[CT]
987 GAATTC
AspHI 1393 GAGCAC G[AT]GC[AT]^C
1903 GTGCTC
AviII 179 TGCGCA TGC^GCA
1229 TGCGCA
BsaHI 292 GACGTC G[GA]^CG[CT]C
1823 GGCGTC
BseRI 1746 CTCCTC GAGGAG(10/8)
1726 GAGGAG
BsiHKAI 1393 GAGCAC G[AT]GC[AT]^C
1903 GTGCTC
BsrI 1053 ACTGG ACTGG(1/-1)
1719 CCAGT
BssHII 1620 GCGCGC G^CGCGC
1849 GCGCGC
BstXI 1166 CCAAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1838 CCAACAAGATGG
DraII 1340 AGGACCT [GA]G^G[ACGT]CC[CT]
1592 AGGCCCT
EarI 204 GAAGAG CTCTTC(1/4)
1087 GAAGAG
Eco47III 38 AGCGCT AGC^GCT
1529 AGCGCT
EcoNI 1332 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1476 CCTCCCAGAGG
EcoO109I 1340 AGGACCT [GA]G^G[ACGT]CC[CT]
1592 AGGCCCT
FspI 179 TGCGCA TGC^GCA
1229 TGCGCA
HgaI 1256 GACGC GACGC(5/10)
1824 GCGTC
Hin1I 292 GACGTC G[GA]^CG[CT]C
1823 GGCGTC
Ksp632I 204 GAAGAG CTCTTC(1/4)
1087 GAAGAG
MseI 458 TTAA T^TAA
790 TTAA
MslI 870 CATAGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1993 CACGAGAATG
PleI 956 GACTC GAGTC(4/5)
1791 GAGTC
Tru9I 458 TTAA T^TAA
790 TTAA
XcmI 590 CCACCTCAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1808 CCACCCTCAGCATGG
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaII BanI BbvI BcnI
BlpI BmyI BpmI Bpu1102I BsaJI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrBI BssKI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII EaeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TseI Tsp509I TspRI TthHB8I XhoII