Restriction map for
pF1KB9742
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AgeI AhdI AlwNI ApaI
ApoI AscI AseI AsnI Asp718I
AspEI AviII AvrII BamHI BbrPI
BbsI BbvCI BcgI BciVI BfaI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BseAI BseRI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraII
DrdI EagI Eam1105I EclXI Eco52I
EcoO109I EcoO65I EcoRI EcoRV FauI
FseI FspI HgaI KpnI KspI
MaeI MamI MfeI MflI MluI
MroI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tsp509I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 18 CGCG CG^CG
AcyI 269 GGCGCC G[GA]^CG[CT]C
AfaI 558 GTAC GT^AC
AflII 738 CTTAAG C^TTAAG
AlwI 293 GGATC GGATC(4/5)
Aor51HI 626 AGCGCT AGC^GCT
AvaI 429 CTCGGG C^[CT]CG[GA]G
BalI 1075 TGGCCA TGG^CCA
BbeI 269 GGCGCC GGCGC^C
BclI 114 TGATCA T^GATCA
BcnI 308 CCCGG CC^[GC]GG
BfrI 738 CTTAAG C^TTAAG
BglI 845 GCCATGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 269 GGCGCC G[GA]^CG[CT]C
BsaWI 644 ACCGGA [AT]^CCGG[AT]
BsoBI 429 CTCGGG C^[CT]CG[GA]G
BspHI 669 TCATGA T^CATGA
BstAPI 227 GCACTTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 18 CGCG CG^CG
BstXI 326 CCACCACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 835 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 988 GAAGAG CTCTTC(1/4)
Eco47III 626 AGCGCT AGC^GCT
EcoNI 24 CCTGTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1558 CCTTGG C^C[AT][AT]GG
EcoT22I 978 ATGCAT ATGCA^T
FbaI 114 TGATCA T^GATCA
Hin1I 269 GGCGCC G[GA]^CG[CT]C
HpaI 1300 GTTAAC GTT^AAC
KasI 269 GGCGCC G^GCGCC
Ksp632I 988 GAAGAG CTCTTC(1/4)
MaeII 793 ACGT A^CGT
MluNI 1075 TGGCCA TGG^CCA
MscI 1075 TGGCCA TGG^CCA
MspA1I 1256 CAGCTG C[AC]G^C[GT]G
MvnI 18 CGCG CG^CG
NarI 269 GGCGCC GG^CGCC
NciI 308 CCCGG CC^[GC]GG
NsiI 978 ATGCAT ATGCA^T
PstI 234 CTGCAG CTGCA^G
PvuII 1256 CAGCTG CAG^CTG
RcaI 669 TCATGA T^CATGA
RsaI 558 GTAC GT^AC
SacI 93 GAGCTC GAGCT^C
SfoI 269 GGCGCC GGC^GCC
SphI 1520 GCATGC GCATG^C
StyI 1558 CCTTGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AocI 154 CCTCAGG CC^T[ACGT]AGG
264 CCTGAGG
Asp700I 540 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
714 GAAAGGCTTC
AspI 477 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1230 GACTATGTC
AvaII 14 GGACC G^G[AT]CC
642 GGACC
BanI 269 GGCGCC G^G[CT][GA]CC
433 GGCACC
BanII 93 GAGCTC G[GA]GC[CT]^C
106 GAGCCC
BsgI 890 GTGCAG GTGCAG(16/14)
1295 GTGCAG
BstNI 30 CCAGG CC^[AT]GG
1078 CCAGG
Bsu36I 154 CCTCAGG CC^T[ACGT]AGG
264 CCTGAGG
DpnII 115 GATC ^GATC
294 GATC
EaeI 310 CGGCCA [CT]^GGCC[GA]
1075 TGGCCA
Eco81I 154 CCTCAGG CC^T[ACGT]AGG
264 CCTGAGG
EcoRII 30 CCAGG ^CC[AT]GG
1078 CCAGG
HaeII 269 GGCGCC [GA]GCGC^[CT]
626 AGCGCT
HapII 309 CCGG C^CGG
645 CCGG
HpaII 309 CCGG C^CGG
645 CCGG
MboI 115 GATC ^GATC
294 GATC
MspI 309 CCGG C^CGG
645 CCGG
MvaI 30 CCAGG CC^[AT]GG
1078 CCAGG
NdeII 115 GATC ^GATC
294 GATC
PflFI 477 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1230 GACTATGTC
PleI 918 GACTC GAGTC(4/5)
1185 GACTC
Sau3AI 115 GATC ^GATC
294 GATC
SfcI 234 CTGCAG C^T[GA][CT]AG
1511 CTGTAG
SmlI 723 CTCAAG C^T[CT][GA]AG
738 CTTAAG
StuI 542 AGGCCT AGG^CCT
727 AGGCCT
TaqI 388 TCGA T^CGA
570 TCGA
TfiI 277 GAATC G^A[AT]TC
1458 GAATC
Tth111I 477 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1230 GACTATGTC
TthHB8I 388 TCGA T^CGA
570 TCGA
XmnI 540 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
714 GAAAGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AflIII AluI Alw44I ApaLI
AspHI BbvI BmyI BsaI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspLU11I BsrDI BsrI BssKI
BstF5I Cac8I CfoI Cfr13I DdeI
DsaI Fnu4HI FokI HaeIII HhaI
HinP1I HincII HindII HindIII HinfI
HphI ItaI MaeIII MboII MnlI
MseI MslI MwoI NlaIII NlaIV
NspI Sau96I ScrFI SfaNI Tru9I
TseI Tsp45I TspRI