Restriction map for
pF1KB9735
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BclI BfrI BglI
BglII BlnI BpuAI BsaAI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspEI BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoRV FbaI FseI
FspI HgaI Hin1I HindIII KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TaqI TthHB8I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 340 GTAGAC GT^[AC][GT]AC
AflIII 284 ACATGT A^C[GA][CT]GT
Aor51HI 1007 AGCGCT AGC^GCT
ApaI 274 GGGCCC GGGCC^C
AspI 127 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 33 CCCGGG C^[CT]CG[GA]G
BanI 681 GGTGCC G^G[CT][GA]CC
BanII 274 GGGCCC G[GA]GC[CT]^C
BciVI 698 GTATCC GTATCC(6/5)
BsgI 1202 CTGCAC GTGCAG(16/14)
BsmBI 5 GAGACG CGTCTC(1/5)
BsoBI 33 CCCGGG C^[CT]CG[GA]G
BspHI 1418 TCATGA T^CATGA
BspLU11I 284 ACATGT A^CATGT
BstEII 405 GGTTACC G^GT[ACGT]ACC
BstPI 405 GGTTACC G^GT[ACGT]ACC
BstXI 845 CCAGCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 987 AGATCC [GA]^GATC[CT]
Eco47III 1007 AGCGCT AGC^GCT
EcoO65I 405 GGTTACC G^GT[ACGT]ACC
EcoRI 229 GAATTC G^AATTC
EcoT22I 589 ATGCAT ATGCA^T
HaeII 1007 AGCGCT [GA]GCGC^[CT]
HincII 715 GTTAAC GT[CT]^[GA]AC
HindII 715 GTTAAC GT[CT]^[GA]AC
HpaI 715 GTTAAC GTT^AAC
MaeII 453 ACGT A^CGT
MflI 987 AGATCC [GA]^GATC[CT]
MseI 716 TTAA T^TAA
NheI 211 GCTAGC G^CTAGC
NsiI 589 ATGCAT ATGCA^T
PflFI 127 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 668 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1418 TCATGA T^CATGA
SapI 325 GAAGAGC GCTCTTC(1/4)
ScaI 952 AGTACT AGT^ACT
SmaI 33 CCCGGG CCC^GGG
SmlI 1220 CTCAAG C^T[CT][GA]AG
SphI 1394 GCATGC GCATG^C
Tru9I 716 TTAA T^TAA
Tth111I 127 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 545 CCAACAGGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 987 AGATCC [GA]^GATC[CT]
XmaCI 33 CCCGGG C^CCGGG
XmaI 33 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 106 AAATTT [GA]^AATT[CT]
229 GAATTC
AlwI 487 GATCC GGATC(4/5)
988 GATCC
ApoI 106 AAATTT [GA]^AATT[CT]
229 GAATTC
AvaII 63 GGACC G^G[AT]CC
1356 GGTCC
BlpI 937 GCTCAGC GC^T[ACGT]AGC
1292 GCTCAGC
BmyI 274 GGGCCC G[AGT]GC[ACT]^C
682 GTGCCC
Bpu1102I 937 GCTCAGC GC^T[ACGT]AGC
1292 GCTCAGC
BsaBI 882 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
982 GATGAAGATC
BsaI 560 GGTCTC GGTCTC(1/5)
927 GGTCTC
BseRI 804 CTCCTC GAGGAG(10/8)
184 GAGGAG
Bsp1286I 274 GGGCCC G[AGT]GC[ACT]^C
682 GTGCCC
CelII 937 GCTCAGC GC^T[ACGT]AGC
1292 GCTCAGC
DraII 274 GGGCCCC [GA]G^G[ACGT]CC[CT]
627 AGGGCCT
DrdI 1189 GACTGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1422 GACCCCACAGTC
DsaI 677 CCATGG C^C[GA][CT]GG
851 CCATGG
EarI 196 CTCTTC CTCTTC(1/4)
325 GAAGAG
EcoO109I 274 GGGCCCC [GA]G^G[ACGT]CC[CT]
627 AGGGCCT
HapII 34 CCGG C^CGG
1026 CCGG
HpaII 34 CCGG C^CGG
1026 CCGG
Ksp632I 196 CTCTTC CTCTTC(1/4)
325 GAAGAG
MamI 882 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
982 GATGAAGATC
MspI 34 CCGG C^CGG
1026 CCGG
NcoI 677 CCATGG C^CATGG
851 CCATGG
PvuII 1117 CAGCTG CAG^CTG
1334 CAGCTG
Tsp509I 107 AATT ^AATT
230 AATT
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwNI BalI
BbvI BcnI BfaI BpmI BsaJI
BsiYI BslI BsmAI BsmFI BsrDI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI MaeI MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI TseI Tsp45I TspRI