Restriction map for
pF1KB9730
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BsaAI BsaBI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspLU11I BsrGI BsrI BssSI Bst1107I
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eam1105I Eco81I EcoRI
EcoRV EcoT22I FbaI HincII HindII
HindIII HpaI KasI KpnI MaeI
MamI MfeI MflI MluI MluNI
MscI MseI MunI NarI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SnaBI SpeI
Sse8387I SspBI StuI SwaI TfiI
Tru9I Tth111I XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 1040 TCCGGA T^CCGGA
AcyI 165 GGCGTC G[GA]^CG[CT]C
AfaI 1385 GTAC GT^AC
Aor51HI 917 AGCGCT AGC^GCT
ApaI 218 GGGCCC GGGCC^C
Asp700I 848 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 992 TGCGCA TGC^GCA
BbsI 1171 GTCTTC GAAGAC(2/6)
BpuAI 1171 GTCTTC GAAGAC(2/6)
BsaHI 165 GGCGTC G[GA]^CG[CT]C
BsaI 1033 GAGACC GGTCTC(1/5)
BseAI 1040 TCCGGA T^CCGGA
BseRI 1314 GAGGAG GAGGAG(10/8)
BsgI 37 CTGCAC GTGCAG(16/14)
BspEI 1040 TCCGGA T^CCGGA
BspHI 1298 TCATGA T^CATGA
BsrDI 842 GCAATG GCAATG(2/0)
BstBI 652 TTCGAA TT^CGAA
BstEII 822 GGTGACC G^GT[ACGT]ACC
BstPI 822 GGTGACC G^GT[ACGT]ACC
DraIII 40 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 868 GAAGAG CTCTTC(1/4)
Eco47III 917 AGCGCT AGC^GCT
EcoNI 1037 CCTTCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 822 GGTGACC G^GT[ACGT]ACC
FseI 237 GGCCGGCC GGCCGG^CC
FspI 992 TGCGCA TGC^GCA
Hin1I 165 GGCGTC G[GA]^CG[CT]C
Ksp632I 868 GAAGAG CTCTTC(1/4)
KspI 120 CCGCGG CCGC^GG
MaeIII 823 GTGAC ^GT[ACGT]AC
MroI 1040 TCCGGA T^CCGGA
NspV 652 TTCGAA TT^CGAA
PpuMI 973 GGGACCC [GA]G^G[AT]CC[CT]
PvuI 26 CGATCG CGAT^CG
RcaI 1298 TCATGA T^CATGA
RsaI 1385 GTAC GT^AC
SacI 910 GAGCTC GAGCT^C
SacII 120 CCGCGG CCGC^GG
SfuI 652 TTCGAA TT^CGAA
SmlI 913 CTCAAG C^T[CT][GA]AG
SspI 1206 AATATT AAT^ATT
Tsp45I 823 GTGAC ^GT[GC]AC
TspRI 1281 CACTG CA[GC]TG[ACGT][ACGT]^
XmnI 848 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 33 enzymes) #
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AvaI 606 CCCGGG C^[CT]CG[GA]G
803 CCCGGG
AvaII 974 GGACC G^G[AT]CC
984 GGACC
BanI 60 GGTGCC G^G[CT][GA]CC
723 GGTGCC
BglI 233 GCCTGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
419 GCCTGGCGGGC
BlpI 1097 GCTTAGC GC^T[ACGT]AGC
1275 GCTGAGC
BpmI 546 CTCCAG CTGGAG(16/14)
1008 CTGGAG
Bpu1102I 1097 GCTTAGC GC^T[ACGT]AGC
1275 GCTGAGC
BsaWI 553 TCCGGT [AT]^CCGG[AT]
1040 TCCGGA
BsmFI 973 GGGAC GGGAC(10/14)
168 GTCCC
BsoBI 606 CCCGGG C^[CT]CG[GA]G
803 CCCGGG
BsrBI 963 CCGCTC CCGCTC(-3/-3)
435 GAGCGG
BssHII 100 GCGCGC G^CGCGC
830 GCGCGC
BstAPI 327 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
948 GCAGAGGGTGC
CelII 1097 GCTTAGC GC^T[ACGT]AGC
1275 GCTGAGC
DpnII 27 GATC ^GATC
873 GATC
DraII 218 GGGCCCC [GA]G^G[ACGT]CC[CT]
973 GGGACCC
EcoO109I 218 GGGCCCC [GA]G^G[ACGT]CC[CT]
973 GGGACCC
HaeII 416 AGCGCC [GA]GCGC^[CT]
917 AGCGCT
HinfI 276 GACTC G^A[ACGT]TC
1317 GAGTC
MaeII 431 ACGT A^CGT
512 ACGT
MboI 27 GATC ^GATC
873 GATC
NdeII 27 GATC ^GATC
873 GATC
PleI 276 GACTC GAGTC(4/5)
1317 GAGTC
PstI 325 CTGCAG CTGCA^G
1060 CTGCAG
PvuII 10 CAGCTG CAG^CTG
1261 CAGCTG
Sau3AI 27 GATC ^GATC
873 GATC
SmaI 606 CCCGGG CCC^GGG
803 CCCGGG
SphI 293 GCATGC GCATG^C
1105 GCATGC
TaqI 653 TCGA T^CGA
1191 TCGA
TthHB8I 653 TCGA T^CGA
1191 TCGA
XcmI 254 CCACCATGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
548 CCAGCTCCGGTCTGG
XmaCI 606 CCCGGG C^CCGGG
803 CCCGGG
XmaI 606 CCCGGG C^CCGGG
803 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
BanII BbvI BcnI BmyI BsaJI
BsiEI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BspMI BsrFI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DsaI EaeI
EagI EclXI Eco52I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HpaII HphI
ItaI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NgoMIV NlaIII NlaIV
NspI PflMI Sau96I ScrFI SfaNI
SfcI StyI TseI Tsp509I Van91I