Restriction map for
pF1KB9716
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BbeI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BseRI BsgI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DrdI Eam1105I Eco47III Eco81I EcoO109I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FspI HincII HindII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MseI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 751 GACGTC GACGT^C
AccII 33 CGCG CG^CG
AccIII 413 TCCGGA T^CCGGA
AflIII 881 ACGTGT A^C[GA][CT]GT
AlwNI 703 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 416 GGACC G^G[AT]CC
BanI 1011 GGTGCC G^G[CT][GA]CC
BbrPI 958 CACGTG CAC^GTG
BbsI 754 GTCTTC GAAGAC(2/6)
BpuAI 754 GTCTTC GAAGAC(2/6)
BsaAI 958 CACGTG [CT]AC^GT[GA]
BsaWI 413 TCCGGA [AT]^CCGG[AT]
BseAI 413 TCCGGA T^CCGGA
BsiEI 84 CGGCCG CG[GA][CT]^CG
BsmBI 390 CGTCTC CGTCTC(1/5)
BspEI 413 TCCGGA T^CCGGA
BspMI 206 GCAGGT ACCTGC(4/8)
BstEII 946 GGTTACC G^GT[ACGT]ACC
BstPI 946 GGTTACC G^GT[ACGT]ACC
BstUI 33 CGCG CG^CG
BstXI 275 CCAAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 180 CCACGG C^C[GA][CT]GG
EagI 84 CGGCCG C^GGCCG
EclXI 84 CGGCCG C^GGCCG
Eco52I 84 CGGCCG C^GGCCG
EcoNI 351 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 946 GGTTACC G^GT[ACGT]ACC
FseI 85 GGCCGGCC GGCCGG^CC
HaeII 523 AGCGCC [GA]GCGC^[CT]
HindIII 713 AAGCTT A^AGCTT
MroI 413 TCCGGA T^CCGGA
MvnI 33 CGCG CG^CG
NaeI 86 GCCGGC GCC^GGC
NgoMIV 86 GCCGGC G^CCGGC
NlaIII 924 CATG CATG^
PleI 843 GAGTC GAGTC(4/5)
PmaCI 958 CACGTG CAC^GTG
PmlI 958 CACGTG CAC^GTG
ScaI 797 AGTACT AGT^ACT
SmaI 1133 CCCGGG CCC^GGG
XmaCI 1133 CCCGGG C^CCGGG
XmaI 1133 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 270 GACGCC G[GA]^CG[CT]C
751 GACGTC
AspHI 769 GAGCAC G[AT]GC[AT]^C
930 GAGCAC
AvaI 64 CTCGGG C^[CT]CG[GA]G
1133 CCCGGG
BalI 480 TGGCCA TGG^CCA
972 TGGCCA
BamHI 544 GGATCC G^GATCC
563 GGATCC
BanII 624 GGGCTC G[GA]GC[CT]^C
1137 GGGCTC
BsaHI 270 GACGCC G[GA]^CG[CT]C
751 GACGTC
BsaI 217 GAGACC GGTCTC(1/5)
341 GAGACC
BsiHKAI 769 GAGCAC G[AT]GC[AT]^C
930 GAGCAC
BsmFI 460 GGGAC GGGAC(10/14)
631 GTCCC
BsoBI 64 CTCGGG C^[CT]CG[GA]G
1133 CCCGGG
BstYI 544 GGATCC [GA]^GATC[CT]
563 GGATCC
CfoI 114 GCGC GCG^C
524 GCGC
EarI 393 CTCTTC CTCTTC(1/4)
594 GAAGAG
FauI 1093 CCCGC CCCGC(4/6)
171 GCGGG
HhaI 114 GCGC GCG^C
524 GCGC
Hin1I 270 GACGCC G[GA]^CG[CT]C
751 GACGTC
HinP1I 114 GCGC G^CGC
524 GCGC
Ksp632I 393 CTCTTC CTCTTC(1/4)
594 GAAGAG
MflI 544 GGATCC [GA]^GATC[CT]
563 GGATCC
MluNI 480 TGGCCA TGG^CCA
972 TGGCCA
MscI 480 TGGCCA TGG^CCA
972 TGGCCA
PvuII 166 CAGCTG CAG^CTG
706 CAGCTG
TaqI 339 TCGA T^CGA
841 TCGA
TthHB8I 339 TCGA T^CGA
841 TCGA
XhoII 544 GGATCC [GA]^GATC[CT]
563 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI Cac8I Cfr10I Cfr13I
DdeI DpnII EaeI EcoRII Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI TfiI TseI Tsp45I TspRI
XcmI