Restriction map for
pF1KB9710
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaII AviII AvrII BamHI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaWI BseAI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DraIII DrdI Eam1105I
EarI Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I MfeI MluI MroI
MseI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tsp45I Tth111I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcyI 706 GGCGCC G[GA]^CG[CT]C
AfaI 810 GTAC GT^AC
AlwI 246 GATCC GGATC(4/5)
Asp700I 789 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 669 CCCGGG C^[CT]CG[GA]G
BanI 706 GGCGCC G^G[CT][GA]CC
BbeI 706 GGCGCC GGCGC^C
BglI 144 GCCCTACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 628 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 706 GGCGCC G[GA]^CG[CT]C
BsaI 688 GAGACC GGTCTC(1/5)
BsmBI 502 GAGACG CGTCTC(1/5)
BsmFI 973 GTCCC GGGAC(10/14)
BsoBI 669 CCCGGG C^[CT]CG[GA]G
BsrDI 923 GCAATG GCAATG(2/0)
BsrI 640 ACTGG ACTGG(1/-1)
BstYI 245 AGATCC [GA]^GATC[CT]
EcoNI 279 CCTGGCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 706 GGCGCC G[GA]^CG[CT]C
KasI 706 GGCGCC G^GCGCC
MaeIII 407 GTAAC ^GT[ACGT]AC
MamI 628 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 245 AGATCC [GA]^GATC[CT]
MslI 135 CATGTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 706 GGCGCC GG^CGCC
PflMI 675 CCACGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 772 CAGCTG CAG^CTG
RsaI 810 GTAC GT^AC
SfoI 706 GGCGCC GGC^GCC
SmaI 669 CCCGGG CCC^GGG
TaqI 413 TCGA T^CGA
Tsp509I 931 AATT ^AATT
TspRI 799 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 413 TCGA T^CGA
Van91I 675 CCACGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 245 AGATCC [GA]^GATC[CT]
XmaCI 669 CCCGGG C^CCGGG
XmaI 669 CCCGGG C^CCGGG
XmnI 789 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 118 GTCTAC GT^[AC][GT]AC
726 GTCTAC
AlwNI 388 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
772 CAGCTGCTG
Aor51HI 216 AGCGCT AGC^GCT
827 AGCGCT
BanII 474 GGGCTC G[GA]GC[CT]^C
946 GAGCCC
BbsI 565 GAAGAC GAAGAC(2/6)
664 GAAGAC
BfaI 784 CTAG C^TAG
1027 CTAG
BmyI 474 GGGCTC G[AGT]GC[ACT]^C
946 GAGCCC
BpuAI 565 GAAGAC GAAGAC(2/6)
664 GAAGAC
BseRI 586 GAGGAG GAGGAG(10/8)
685 GAGGAG
BsgI 508 GTGCAG GTGCAG(16/14)
865 GTGCAG
BsiEI 151 CGGCCG CG[GA][CT]^CG
497 CGGCCG
BsmAI 502 GAGAC GTCTC(1/5)
688 GAGAC
Bsp1286I 474 GGGCTC G[AGT]GC[ACT]^C
946 GAGCCC
BspMI 867 GCAGGT ACCTGC(4/8)
995 GCAGGT
BstAPI 387 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
919 GCAGGCAATGC
BstF5I 978 CATCC GGATG(2/0)
627 GGATG
DdeI 856 CTCAG C^T[ACGT]AG
962 CTAAG
DsaI 494 CCGCGG C^C[GA][CT]GG
710 CCGCGG
EagI 151 CGGCCG C^GGCCG
497 CGGCCG
EclXI 151 CGGCCG C^GGCCG
497 CGGCCG
Eco47III 216 AGCGCT AGC^GCT
827 AGCGCT
Eco52I 151 CGGCCG C^GGCCG
497 CGGCCG
FokI 978 CATCC GGATG(9/13)
627 GGATG
HgaI 439 GACGC GACGC(5/10)
340 GCGTC
HinfI 73 GACTC G^A[ACGT]TC
553 GAGTC
KspI 494 CCGCGG CCGC^GG
710 CCGCGG
MaeI 784 CTAG C^TAG
1027 CTAG
MaeII 724 ACGT A^CGT
989 ACGT
MboII 565 GAAGA GAAGA(8/7)
664 GAAGA
PleI 73 GACTC GAGTC(4/5)
553 GAGTC
PstI 208 CTGCAG CTGCA^G
430 CTGCAG
SacII 494 CCGCGG CCGC^GG
710 CCGCGG
SfaNI 33 GATGC GCATC(5/9)
1017 GCATC
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AccII AciI AluI BalI BbvI
BcnI BpmI BsaJI BsiYI BslI
BsrFI BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DpnII EaeI
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfcI TseI