Restriction map for
pF1KB9707
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AgeI AhdI AlwI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AvrII BalI BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BpuAI
BsaBI BsaI BseRI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrDI BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I Bsu36I ClaI DraI DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HpaI MamI MfeI MflI
MluI MluNI MscI MseI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI SalI
SbfI ScaI SexAI SfcI SfiI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp45I Tth111I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 623 GGTACC G^GTACC
AccIII 987 TCCGGA T^CCGGA
AflIII 947 ACACGT A^C[GA][CT]GT
Alw44I 285 GTGCAC G^TGCAC
AlwNI 319 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 285 GTGCAC G^TGCAC
Asp718I 623 GGTACC G^GTACC
AviII 923 TGCGCA TGC^GCA
BfaI 979 CTAG C^TAG
BglI 679 GCCAGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 948 CACGTA [CT]AC^GT[GA]
BsaWI 987 TCCGGA [AT]^CCGG[AT]
BseAI 987 TCCGGA T^CCGGA
BsgI 208 CTGCAC GTGCAG(16/14)
BspEI 987 TCCGGA T^CCGGA
BsrFI 364 GCCGGC [GA]^CCGG[CT]
BssHII 429 GCGCGC G^CGCGC
BssSI 420 CTCGTG CACGAG(-5/-1)
BstXI 821 CCAAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 364 GCCGGC [GA]^CCGG[CT]
CpoI 671 CGGACCG CG^G[AT]CCG
DpnII 100 GATC ^GATC
DraIII 417 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 251 CTCTTC CTCTTC(1/4)
FspI 923 TGCGCA TGC^GCA
HgaI 395 GCGTC GACGC(5/10)
HindIII 174 AAGCTT A^AGCTT
HphI 641 TCACC GGTGA(8/7)
KpnI 623 GGTACC GGTAC^C
Ksp632I 251 CTCTTC CTCTTC(1/4)
MaeI 979 CTAG C^TAG
MaeII 949 ACGT A^CGT
MaeIII 204 GTTAC ^GT[ACGT]AC
MboI 100 GATC ^GATC
MboII 252 TCTTC GAAGA(8/7)
MroI 987 TCCGGA T^CCGGA
MslI 595 CATGCGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 364 GCCGGC GCC^GGC
NcoI 223 CCATGG C^CATGG
NdeII 100 GATC ^GATC
NgoMIV 364 GCCGGC G^CCGGC
PpuMI 714 AGGTCCC [GA]G^G[AT]CC[CT]
RsrII 671 CGGACCG CG^G[AT]CCG
SacI 630 GAGCTC GAGCT^C
SapI 250 GCTCTTC GCTCTTC(1/4)
Sau3AI 100 GATC ^GATC
SphI 594 GCATGC GCATG^C
TaqI 690 TCGA T^CGA
TspRI 501 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 690 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 310 GGCGCC G[GA]^CG[CT]C
376 GGCGCC
AspHI 285 GTGCAC G[AT]GC[AT]^C
630 GAGCTC
AvaI 345 CCCGGG C^[CT]CG[GA]G
585 CCCGGG
BbeI 310 GGCGCC GGCGC^C
376 GGCGCC
BlpI 632 GCTCAGC GC^T[ACGT]AGC
882 GCTGAGC
BpmI 44 CTCCAG CTGGAG(16/14)
742 CTGGAG
Bpu1102I 632 GCTCAGC GC^T[ACGT]AGC
882 GCTGAGC
BsaHI 310 GGCGCC G[GA]^CG[CT]C
376 GGCGCC
BsiHKAI 285 GTGCAC G[AT]GC[AT]^C
630 GAGCTC
BsmAI 139 GAGAC GTCTC(1/5)
1018 GAGAC
BsoBI 345 CCCGGG C^[CT]CG[GA]G
585 CCCGGG
BsrBI 306 GAGCGG CCGCTC(-3/-3)
930 GAGCGG
CelII 632 GCTCAGC GC^T[ACGT]AGC
882 GCTGAGC
EcoT14I 111 CCAAGG C^C[AT][AT]GG
223 CCATGG
HaeII 310 GGCGCC [GA]GCGC^[CT]
376 GGCGCC
Hin1I 310 GGCGCC G[GA]^CG[CT]C
376 GGCGCC
HinfI 241 GACTC G^A[ACGT]TC
298 GACTC
KasI 310 GGCGCC G^GCGCC
376 GGCGCC
KspI 380 CCGCGG CCGC^GG
554 CCGCGG
NarI 310 GGCGCC GG^CGCC
376 GGCGCC
NspI 142 ACATGC [GA]CATG^[CT]
594 GCATGC
PleI 241 GACTC GAGTC(4/5)
298 GACTC
SacII 380 CCGCGG CCGC^GG
554 CCGCGG
SfoI 310 GGCGCC GGC^GCC
376 GGCGCC
SmaI 345 CCCGGG CCC^GGG
585 CCCGGG
StyI 111 CCAAGG C^C[AT][AT]GG
223 CCATGG
Tsp509I 80 AATT ^AATT
871 AATT
XcmI 181 CCACAGCTGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
214 CCAGAAGTTCCATGG
XmaCI 345 CCCGGG C^CCGGG
585 CCCGGG
XmaI 345 CCCGGG C^CCGGG
585 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AfaI AluI ApaI
AvaII BanI BanII BbvI BcnI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraII DsaI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII ItaI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV PflMI PvuII RsaI
Sau96I ScrFI SfaNI TseI Van91I