Restriction map for
pF1KB9697
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BamHI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HgaI HincII HindII HindIII HinfI
HpaI KpnI Ksp632I MaeI MamI
MfeI MluI MroI MseI MslI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp45I Tsp509I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AcyI 794 GGCGCC G[GA]^CG[CT]C
Alw44I 172 GTGCAC G^TGCAC
ApaLI 172 GTGCAC G^TGCAC
AvaI 463 CCCGGG C^[CT]CG[GA]G
AvaII 531 GGACC G^G[AT]CC
AviII 695 TGCGCA TGC^GCA
BalI 321 TGGCCA TGG^CCA
BbeI 794 GGCGCC GGCGC^C
BbrPI 169 CACGTG CAC^GTG
BbsI 52 GAAGAC GAAGAC(2/6)
BglI 773 GCCGCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 52 GAAGAC GAAGAC(2/6)
BsaAI 169 CACGTG [CT]AC^GT[GA]
BsaHI 794 GGCGCC G[GA]^CG[CT]C
BsaI 330 GGTCTC GGTCTC(1/5)
BsgI 226 GTGCAG GTGCAG(16/14)
BsoBI 463 CCCGGG C^[CT]CG[GA]G
BssSI 97 CACGAG CACGAG(-5/-1)
DraII 779 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 166 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 779 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 296 CCATGG C^C[AT][AT]GG
FspI 695 TGCGCA TGC^GCA
Hin1I 794 GGCGCC G[GA]^CG[CT]C
KasI 794 GGCGCC G^GCGCC
KspI 774 CCGCGG CCGC^GG
MaeIII 3 GTTAC ^GT[ACGT]AC
MluNI 321 TGGCCA TGG^CCA
MscI 321 TGGCCA TGG^CCA
NarI 794 GGCGCC GG^CGCC
NcoI 296 CCATGG C^CATGG
PflMI 675 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 169 CACGTG CAC^GTG
PmlI 169 CACGTG CAC^GTG
PstI 241 CTGCAG CTGCA^G
PvuII 238 CAGCTG CAG^CTG
SacI 214 GAGCTC GAGCT^C
SacII 774 CCGCGG CCGC^GG
SfcI 241 CTGCAG C^T[GA][CT]AG
SfiI 772 GGCCGCGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 794 GGCGCC GGC^GCC
SmaI 463 CCCGGG CCC^GGG
StyI 296 CCATGG C^C[AT][AT]GG
Van91I 675 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 309 CCATCCCAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 463 CCCGGG C^CCGGG
XmaI 463 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AfaI 360 GTAC GT^AC
516 GTAC
AlwI 507 GATCC GGATC(4/5)
75 GGATC
AlwNI 520 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
561 CAGCATCTG
Asp700I 22 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
201 GAACAACTTC
AspHI 172 GTGCAC G[AT]GC[AT]^C
214 GAGCTC
BseRI 397 GAGGAG GAGGAG(10/8)
400 GAGGAG
BsiHKAI 172 GTGCAC G[AT]GC[AT]^C
214 GAGCTC
BsmAI 487 GAGAC GTCTC(1/5)
331 GTCTC
BspMI 338 ACCTGC ACCTGC(4/8)
533 ACCTGC
BsrBI 409 GAGCGG CCGCTC(-3/-3)
790 GAGCGG
BstYI 75 GGATCT [GA]^GATC[CT]
506 AGATCC
DdeI 115 CTCAG C^T[ACGT]AG
390 CTCAG
DpnII 76 GATC ^GATC
507 GATC
DsaI 296 CCATGG C^C[GA][CT]GG
774 CCGCGG
EaeI 321 TGGCCA [CT]^GGCC[GA]
785 TGGCCG
HaeII 621 GGCGCT [GA]GCGC^[CT]
794 GGCGCC
HapII 345 CCGG C^CGG
464 CCGG
HpaII 345 CCGG C^CGG
464 CCGG
HphI 729 GGTGA GGTGA(8/7)
743 TCACC
MaeII 170 ACGT A^CGT
281 ACGT
MboI 76 GATC ^GATC
507 GATC
MflI 75 GGATCT [GA]^GATC[CT]
506 AGATCC
MspI 345 CCGG C^CGG
464 CCGG
NdeII 76 GATC ^GATC
507 GATC
RsaI 360 GTAC GT^AC
516 GTAC
Sau3AI 76 GATC ^GATC
507 GATC
SexAI 56 ACCTGGT A^CC[AT]GGT
326 ACCAGGT
TaqI 185 TCGA T^CGA
287 TCGA
TspRI 120 CAGTG CA[GC]TG[ACGT][ACGT]^
425 CACTG
TthHB8I 185 TCGA T^CGA
287 TCGA
XhoII 75 GGATCT [GA]^GATC[CT]
506 AGATCC
XmnI 22 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
201 GAACAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AccII AciI AluI BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssHII BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I EcoRII FauI
Fnu4HI FokI HaeIII HhaI HinP1I
ItaI MboII MnlI MspA1I MvaI
MvnI MwoI NciI NlaIII NlaIV
Sau96I ScrFI SfaNI TseI