Restriction map for
pF1KB9689
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AfaI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI ApaI ApoI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BalI BamHI BbrPI
BbsI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BseRI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
MaeII MamI MfeI MluI MluNI
MscI MseI MslI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SalI SbfI ScaI SexAI
SfcI SfiI SfuI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tsp509I Tth111I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 143 TCCGGA T^CCGGA
Alw44I 505 GTGCAC G^TGCAC
ApaLI 505 GTGCAC G^TGCAC
AscI 577 GGCGCGCC GG^CGCGCC
AspHI 505 GTGCAC G[AT]GC[AT]^C
AviII 532 TGCGCA TGC^GCA
BanII 217 GAGCCC G[GA]GC[CT]^C
BbvCI 102 GCTGAGG CCTCAGC(-5/-2)
BfaI 521 CTAG C^TAG
BsaI 678 GAGACC GGTCTC(1/5)
BseAI 143 TCCGGA T^CCGGA
BsiHKAI 505 GTGCAC G[AT]GC[AT]^C
BsmAI 678 GAGAC GTCTC(1/5)
BspEI 143 TCCGGA T^CCGGA
BsrBI 121 GAGCGG CCGCTC(-3/-3)
BstEII 3 GGTCACC G^GT[ACGT]ACC
BstPI 3 GGTCACC G^GT[ACGT]ACC
BstYI 423 AGATCC [GA]^GATC[CT]
DraII 647 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 647 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 3 GGTCACC G^GT[ACGT]ACC
FspI 532 TGCGCA TGC^GCA
HinfI 571 GATTC G^A[ACGT]TC
KspI 230 CCGCGG CCGC^GG
MaeI 521 CTAG C^TAG
MaeIII 4 GTCAC ^GT[ACGT]AC
MflI 423 AGATCC [GA]^GATC[CT]
MroI 143 TCCGGA T^CCGGA
NaeI 582 GCCGGC GCC^GGC
NgoMIV 582 GCCGGC G^CCGGC
NlaIII 536 CATG CATG^
PvuII 282 CAGCTG CAG^CTG
SacII 230 CCGCGG CCGC^GG
SgrAI 581 CGCCGGCG C[GA]^CCGG[CT]G
StuI 430 AGGCCT AGG^CCT
TaqI 15 TCGA T^CGA
TfiI 571 GATTC G^A[AT]TC
Tsp45I 4 GTCAC ^GT[GC]AC
TspRI 503 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 15 TCGA T^CGA
XhoII 423 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwI 424 GATCC GGATC(4/5)
12 GGATC
AvaI 8 CCCGGG C^[CT]CG[GA]G
128 CCCGGG
BmyI 217 GAGCCC G[AGT]GC[ACT]^C
505 GTGCAC
BsgI 379 CTGCAC GTGCAG(16/14)
487 CTGCAC
BsiEI 224 CGGCCG CG[GA][CT]^CG
350 CGGCCG
BsoBI 8 CCCGGG C^[CT]CG[GA]G
128 CCCGGG
Bsp1286I 217 GAGCCC G[AGT]GC[ACT]^C
505 GTGCAC
BsrI 256 CCAGT ACTGG(1/-1)
502 CCAGT
BssHII 561 GCGCGC G^CGCGC
578 GCGCGC
BstF5I 449 CATCC GGATG(2/0)
545 CATCC
DdeI 20 CTGAG C^T[ACGT]AG
103 CTGAG
DpnII 13 GATC ^GATC
424 GATC
DsaI 185 CCGTGG C^C[GA][CT]GG
230 CCGCGG
EaeI 224 CGGCCG [CT]^GGCC[GA]
350 CGGCCG
EagI 224 CGGCCG C^GGCCG
350 CGGCCG
EarI 367 CTCTTC CTCTTC(1/4)
97 GAAGAG
EclXI 224 CGGCCG C^GGCCG
350 CGGCCG
Eco52I 224 CGGCCG C^GGCCG
350 CGGCCG
FokI 449 CATCC GGATG(9/13)
545 CATCC
HgaI 265 GACGC GACGC(5/10)
340 GACGC
HphI 5 TCACC GGTGA(8/7)
593 TCACC
Ksp632I 367 CTCTTC CTCTTC(1/4)
97 GAAGAG
MboI 13 GATC ^GATC
424 GATC
MboII 97 GAAGA GAAGA(8/7)
368 TCTTC
NdeII 13 GATC ^GATC
424 GATC
SapI 97 GAAGAGC GCTCTTC(1/4)
366 GCTCTTC
Sau3AI 13 GATC ^GATC
424 GATC
SfaNI 42 GATGC GCATC(5/9)
80 GCATC
SmaI 8 CCCGGG CCC^GGG
128 CCCGGG
SmlI 46 CTCAAG C^T[CT][GA]AG
195 CTTGAG
XmaCI 8 CCCGGG C^CCGGG
128 CCCGGG
XmaI 8 CCCGGG C^CCGGG
128 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AcyI AluI AvaII
BanI BbeI BbvI BcnI BglI
BpmI BsaHI BsaJI BsaWI BsiYI
BslI BsmFI BsrFI BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
EcoRII EcoT14I FauI Fnu4HI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII ItaI KasI MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NlaIV Sau96I ScrFI SfoI
StyI TseI