Restriction map for
pF1KB9688
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BciVI BclI
BfaI BglI BlnI BlpI Bpu1102I
BpuAI BsaHI BsaI BsaWI BseAI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BspMI BsrDI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DraIII DrdI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I MaeI MfeI MluI MluNI
MroI MscI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SpeI SphI Sse8387I SspI
StuI StyI SwaI Tsp45I Tth111I
XbaI XcmI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 181 GTAGAC GT^[AC][GT]AC
AcsI 517 GAATTC [GA]^AATT[CT]
AflII 132 CTTAAG C^TTAAG
AlwI 82 GGATC GGATC(4/5)
ApoI 517 GAATTC [GA]^AATT[CT]
Asp700I 513 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 3 GAGCTC G[AT]GC[AT]^C
AvaII 470 GGTCC G^G[AT]CC
BcgI 554 GCATAGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 132 CTTAAG C^TTAAG
BglII 599 AGATCT A^GATCT
BpmI 322 CTGGAG CTGGAG(16/14)
BsaAI 10 TACGTA [CT]AC^GT[GA]
BsaBI 594 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 3 GAGCTC G[AT]GC[AT]^C
Bsp1407I 293 TGTACA T^GTACA
BsrBI 239 CCGCTC CCGCTC(-3/-3)
BsrFI 402 ACCGGC [GA]^CCGG[CT]
BsrGI 293 TGTACA T^GTACA
BsrI 479 CCAGT ACTGG(1/-1)
BssSI 451 CACGAG CACGAG(-5/-1)
BstNI 107 CCAGG CC^[AT]GG
BstYI 599 AGATCT [GA]^GATC[CT]
Cfr10I 402 ACCGGC [GA]^CCGG[CT]
EaeI 236 CGGCCG [CT]^GGCC[GA]
EagI 236 CGGCCG C^GGCCG
EclXI 236 CGGCCG C^GGCCG
Eco52I 236 CGGCCG C^GGCCG
EcoRI 517 GAATTC G^AATTC
EcoRII 107 CCAGG ^CC[AT]GG
HphI 207 TCACC GGTGA(8/7)
KspI 233 CCGCGG CCGC^GG
MaeII 11 ACGT A^CGT
MaeIII 482 GTTAC ^GT[ACGT]AC
MamI 594 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 630 GAAGA GAAGA(8/7)
MflI 599 AGATCT [GA]^GATC[CT]
MspA1I 233 CCGCGG C[AC]G^C[GT]G
MvaI 107 CCAGG CC^[AT]GG
NotI 235 GCGGCCGC GC^GGCCGC
NspI 59 ACATGC [GA]CATG^[CT]
PaeR7I 508 CTCGAG C^TCGAG
PflMI 495 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 498 GACTC GAGTC(4/5)
PstI 225 CTGCAG CTGCA^G
SacI 3 GAGCTC GAGCT^C
SacII 233 CCGCGG CCGC^GG
SfcI 225 CTGCAG C^T[GA][CT]AG
SnaBI 10 TACGTA TAC^GTA
SspBI 293 TGTACA T^GTACA
TaqI 509 TCGA T^CGA
TfiI 637 GATTC G^A[AT]TC
TspRI 342 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 509 TCGA T^CGA
Van91I 495 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 508 CTCGAG C^TCGAG
XhoII 599 AGATCT [GA]^GATC[CT]
XmnI 513 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 234 CGCG CG^CG
488 CGCG
AfaI 111 GTAC GT^AC
294 GTAC
AluI 4 AGCT AG^CT
333 AGCT
AlwNI 108 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
496 CAGACTCTG
BsgI 439 CTGCAC GTGCAG(16/14)
97 GTGCAG
BsiEI 213 CGACCG CG[GA][CT]^CG
236 CGGCCG
BsmBI 263 GAGACG CGTCTC(1/5)
454 GAGACG
BsmFI 246 GGGAC GGGAC(10/14)
471 GTCCC
BstUI 234 CGCG CG^CG
488 CGCG
Cfr13I 405 GGCCC G^G[ACGT]CC
470 GGTCC
HaeII 229 AGCGCC [GA]GCGC^[CT]
318 GGCGCT
HinfI 498 GACTC G^A[ACGT]TC
637 GATTC
MseI 133 TTAA T^TAA
527 TTAA
MvnI 234 CGCG CG^CG
488 CGCG
RsaI 111 GTAC GT^AC
294 GTAC
Sau96I 405 GGCCC G^G[ACGT]CC
470 GGTCC
SfaNI 103 GCATC GCATC(5/9)
137 GCATC
SmaI 200 CCCGGG CCC^GGG
286 CCCGGG
SmlI 132 CTTAAG C^T[CT][GA]AG
508 CTCGAG
Tru9I 133 TTAA T^TAA
527 TTAA
Tsp509I 19 AATT ^AATT
518 AATT
XmaCI 200 CCCGGG C^CCGGG
286 CCCGGG
XmaI 200 CCCGGG C^CCGGG
286 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AvaI BanII BbvI BcnI
BmyI BsaJI BseRI BsiYI BslI
BsmAI BsoBI Bsp1286I BssKI BstF5I
Cac8I CfoI DdeI DpnII DsaI
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MboI
MnlI MspI MwoI NciI NdeII
NlaIII NlaIV Sau3AI ScrFI TseI