##################################
# NO CUT ENZYMES (  205 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstF5I      BstNI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DdeI        
DraI        DraII       DraIII      DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoRI       
EcoRII      EcoRV       EcoT14I     EcoT22I     FbaI        
FokI        FseI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HpaI        HpaII       KasI        
KpnI        Ksp632I     MaeI        MaeII       MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MspI        MunI        MvaI        
NaeI        NarI        NciI        NcoI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PstI        PvuI        
RsrII       SacI        SalI        SapI        Sau96I      
SbfI        ScaI        ScrFI       SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TspRI       Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AccII         231  CGCG                 CG^CG
AcsI          322  AAATTT               [GA]^AATT[CT]
AfaI          177  GTAC                 GT^AC
ApoI          322  AAATTT               [GA]^AATT[CT]
Asp700I       111  GAACCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvI          130  GCTGC                GCAGC(8/12)
BsaJI         230  CCGCGG               C^C[ACGT][ACGT]GG
BspHI         258  TCATGA               T^CATGA
BstEII         31  GGTCACC              G^GT[ACGT]ACC
BstPI          31  GGTCACC              G^GT[ACGT]ACC
BstUI         231  CGCG                 CG^CG
Cac8I          69  GCCAGC               GC[ACGT]^[ACGT]GC
DpnII         365  GATC                 ^GATC
DsaI          230  CCGCGG               C^C[GA][CT]GG
EcoO65I        31  GGTCACC              G^GT[ACGT]ACC
FauI          232  GCGGG                CCCGC(4/6)
Fnu4HI        130  GCTGC                GC^[ACGT]GC
HindIII         9  AAGCTT               A^AGCTT
ItaI          130  GCTGC                GC^[ACGT]GC
KspI          230  CCGCGG               CCGC^GG
MboI          365  GATC                 ^GATC
MseI          415  TTAA                 T^TAA
MvnI          231  CGCG                 CG^CG
NdeI          238  CATATG               CA^TATG
NdeII         365  GATC                 ^GATC
PshAI          97  GACCATCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII          71  CAGCTG               CAG^CTG
RcaI          258  TCATGA               T^CATGA
RsaI          177  GTAC                 GT^AC
SacII         230  CCGCGG               CCGC^GG
Sau3AI        365  GATC                 ^GATC
Tru9I         415  TTAA                 T^TAA
TseI          130  GCTGC                G^C[AT]GC
XmnI          111  GAACCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AciI          230  CCGC                 CCGC(-3/-1)
              232  GCGG                
AflIII        155  ACATGT               A^C[GA][CT]GT
              173  ACATGT              
BspLU11I      155  ACATGT               A^CATGT
              173  ACATGT              
BsrI           46  CCAGT                ACTGG(1/-1)
              114  CCAGT               
HinfI          85  GAATC                G^A[ACGT]TC
              333  GAATC               
HphI           33  TCACC                GGTGA(8/7)
              227  TCACC               
MaeIII         32  GTCAC                ^GT[ACGT]AC
              226  GTCAC               
MboII         274  GAAGA                GAAGA(8/7)
              402  GAAGA               
MnlI          141  CCTC                 CCTC(7/6)
              243  GAGG                
MspA1I         71  CAGCTG               C[AC]G^C[GT]G
              230  CCGCGG              
MwoI           73  GCTGGGAAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              124  GCTCATGCTGC         
NspI          155  ACATGT               [GA]CATG^[CT]
              173  ACATGT              
TfiI           85  GAATC                G^A[AT]TC
              333  GAATC               
Tsp45I         32  GTCAC                ^GT[GC]AC
              226  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

AluI        NlaIII      TaqI        Tsp509I     TthHB8I