##################################
# NO CUT ENZYMES (  225 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BbvI        BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaJI       BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DpnII       DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        Fnu4HI      FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
HphI        ItaI        KasI        KpnI        KspI        
MaeII       MaeIII      MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MspI        MunI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        Sau3AI      Sau96I      SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   23 enzymes) #
###################################

AluI          101  AGCT                 AG^CT
AocI           73  CCTGAGG              CC^T[ACGT]AGG
BfaI          103  CTAG                 C^TAG
BpmI          157  CTCCAG               CTGGAG(16/14)
BsrDI         197  GCAATG               GCAATG(2/0)
BssKI         159  CCAGG                ^CC[ACGT]GG
BstNI         159  CCAGG                CC^[AT]GG
Bsu36I         73  CCTGAGG              CC^T[ACGT]AGG
DdeI           74  CTGAG                C^T[ACGT]AG
DraI          166  TTTAAA               TTT^AAA
Eco81I         73  CCTGAGG              CC^T[ACGT]AGG
EcoRII        159  CCAGG                ^CC[AT]GG
HaeIII        114  GGCC                 GG^CC
MaeI          103  CTAG                 C^TAG
MvaI          159  CCAGG                CC^[AT]GG
NlaIII         55  CATG                 CATG^
PleI          245  GAGTC                GAGTC(4/5)
SapI           61  GAAGAGC              GCTCTTC(1/4)
ScrFI         159  CCAGG                CC^[ACGT]GG
SfaNI         267  GATGC                GCATC(5/9)
TaqI          285  TCGA                 T^CGA
TfiI          147  GATTC                G^A[AT]TC
TthHB8I       285  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    5 enzymes) #
###################################

BstF5I        292  CATCC                GGATG(2/0)
              222  GGATG               
EarI           61  GAAGAG               CTCTTC(1/4)
               88  GAAGAG              
FokI          292  CATCC                GGATG(9/13)
              222  GGATG               
HinfI         147  GATTC                G^A[ACGT]TC
              245  GAGTC               
Ksp632I        61  GAAGAG               CTCTTC(1/4)
               88  GAAGAG              


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

BseRI       MboII       MnlI        MseI        Tru9I