##################################
# NO CUT ENZYMES (  174 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BcnI        BfrI        BglI        BlnI        BlpI        
BmyI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsoBI       Bsp1286I    BspDI       BspEI       BspHI       
BspMI       BsrBI       BsrDI       BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstNI       BstPI       BstXI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr13I      ClaI        
CpoI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRII      EcoRV       EcoT14I     FseI        FspI        
HaeII       HhaI        Hin1I       HinP1I      HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MspA1I      
MunI        MvaI        MwoI        NaeI        NarI        
NciI        NcoI        NgoMIV      NheI        NlaIV       
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScaI        ScrFI       SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    StyI        
TaqI        Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccII        1483  CGCG                 CG^CG
AflIII       1540  ACATGT               A^C[GA][CT]GT
AlwI         1431  GATCC                GGATC(4/5)
BglII         383  AGATCT               A^GATCT
BsaWI         598  ACCGGA               [AT]^CCGG[AT]
BsmAI         352  GAGAC                GTCTC(1/5)
BsmBI         352  GAGACG               CGTCTC(1/5)
BsmFI         265  GGGAC                GGGAC(10/14)
BsmI         1141  GCATTC               GAATGC(1/-1)
BspLU11I     1540  ACATGT               A^CATGT
BsrFI        1102  GCCGGT               [GA]^CCGG[CT]
BstUI        1483  CGCG                 CG^CG
BstYI         383  AGATCT               [GA]^GATC[CT]
Cfr10I       1102  GCCGGT               [GA]^CCGG[CT]
DdeI          153  CTTAG                C^T[ACGT]AG
DraI          361  TTTAAA               TTT^AAA
EarI         1477  CTCTTC               CTCTTC(1/4)
EcoT22I         1  ATGCAT               ATGCA^T
FauI           60  CCCGC                CCCGC(4/6)
HaeIII        300  GGCC                 GG^CC
HgaI          354  GACGC                GACGC(5/10)
HincII        139  GTCAAC               GT[CT]^[GA]AC
HindII        139  GTCAAC               GT[CT]^[GA]AC
HphI         1105  GGTGA                GGTGA(8/7)
Ksp632I      1477  CTCTTC               CTCTTC(1/4)
MflI          383  AGATCT               [GA]^GATC[CT]
MnlI         1413  CCTC                 CCTC(7/6)
MvnI         1483  CGCG                 CG^CG
NdeI          209  CATATG               CA^TATG
NsiI            1  ATGCAT               ATGCA^T
NspI         1540  ACATGT               [GA]CATG^[CT]
PstI           82  CTGCAG               CTGCA^G
SfcI           82  CTGCAG               C^T[GA][CT]AG
SspI          259  AATATT               AAT^ATT
StuI          299  AGGCCT               AGG^CCT
SwaI          360  ATTTAAAT             ATTT^AAAT
XhoII         383  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AciI           61  CCGC                 CCGC(-3/-1)
             1482  CCGC                
BbvI           30  GCAGC                GCAGC(8/12)
               84  GCAGC               
BclI          120  TGATCA               T^GATCA
              755  TGATCA              
Bsp1407I      744  TGTACA               T^GTACA
             1164  TGTACA              
BsrGI         744  TGTACA               T^GTACA
             1164  TGTACA              
FbaI          120  TGATCA               T^GATCA
              755  TGATCA              
Fnu4HI         30  GCAGC                GC^[ACGT]GC
               84  GCAGC               
HindIII      1280  AAGCTT               A^AGCTT
             1391  AAGCTT              
ItaI           30  GCAGC                GC^[ACGT]GC
               84  GCAGC               
MaeII         171  ACGT                 A^CGT
             1503  ACGT                
SfaNI          19  GATGC                GCATC(5/9)
              498  GCATC               
SspBI         744  TGTACA               T^GTACA
             1164  TGTACA              
TseI           30  GCAGC                G^C[AT]GC
               84  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        Asp700I     
BbsI        BfaI        BpmI        BpuAI       BsrI        
BstF5I      DpnII       EcoRI       FokI        HapII       
HinfI       HpaII       MaeI        MaeIII      MboI        
MboII       MseI        MslI        MspI        NdeII       
NlaIII      RsaI        Sau3AI      TfiI        Tru9I       
Tsp45I      Tsp509I     TspRI       XmnI