##################################
# NO CUT ENZYMES (  206 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwI        AlwNI       Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BspDI       
BspEI       BspHI       BspMI       BsrBI       BsrFI       
BssHII      BssKI       Bst1107I    BstAPI      BstBI       
BstEII      BstNI       BstPI       BstUI       BstXI       
BstYI       BstZ17I     Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DpnII       
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT14I     FauI        
FbaI        Fnu4HI      FseI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        HpaII       
ItaI        KasI        KpnI        Ksp632I     KspI        
MamI        MboI        MflI        MluI        MluNI       
MroI        MscI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeII       NgoMIV      NheI        NlaIV       
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
Sau96I      SbfI        ScaI        ScrFI       SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    StuI        StyI        SwaI        
TaqI        TseI        Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   22 enzymes) #
###################################

AciI          149  GCGG                 CCGC(-3/-1)
AflIII        166  ACATGT               A^C[GA][CT]GT
AluI          606  AGCT                 AG^CT
AocI          356  CCTTAGG              CC^T[ACGT]AGG
BsiYI          92  CCCTTCATAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           92  CCCTTCATAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspLU11I      166  ACATGT               A^CATGT
BssSI         721  CACGAG               CACGAG(-5/-1)
BstF5I         89  CATCC                GGATG(2/0)
Bsu36I        356  CCTTAGG              CC^T[ACGT]AGG
Eco81I        356  CCTTAGG              CC^T[ACGT]AGG
EcoT22I        54  ATGCAT               ATGCA^T
FokI           89  CATCC                GGATG(9/13)
HindIII       605  AAGCTT               A^AGCTT
MaeII          81  ACGT                 A^CGT
MnlI          142  GAGG                 CCTC(7/6)
NdeI          131  CATATG               CA^TATG
NsiI           54  ATGCAT               ATGCA^T
NspI          166  ACATGT               [GA]CATG^[CT]
PleI          813  GACTC                GAGTC(4/5)
SspI          277  AATATT               AAT^ATT
TfiI          393  GATTC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

BfaI           35  CTAG                 C^TAG
              872  CTAG                
BsmAI         215  GAGAC                GTCTC(1/5)
              617  GTCTC               
BsmI           52  GAATGC               GAATGC(1/-1)
              145  GAATGC              
Bsp1407I      163  TGTACA               T^GTACA
              253  TGTACA              
BsrDI         426  GCAATG               GCAATG(2/0)
              678  GCAATG              
BsrGI         163  TGTACA               T^GTACA
              253  TGTACA              
DdeI          357  CTTAG                C^T[ACGT]AG
              619  CTCAG               
HinfI         393  GATTC                G^A[ACGT]TC
              813  GACTC               
HphI          655  GGTGA                GGTGA(8/7)
              456  TCACC               
MaeI           35  CTAG                 C^TAG
              872  CTAG                
MboII         658  GAAGA                GAAGA(8/7)
               74  TCTTC               
MfeI          537  CAATTG               C^AATTG
              762  CAATTG              
MunI          537  CAATTG               C^AATTG
              762  CAATTG              
SspBI         163  TGTACA               T^GTACA
              253  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (   16 enzymes) #
#########################################

AcsI        AfaI        ApoI        Asp700I     BpmI        
BsrI        MaeIII      MseI        MslI        NlaIII      
RsaI        Tru9I       Tsp45I      Tsp509I     TspRI       
XmnI