Restriction map for
pF1KB9651
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII BalI
BamHI BanII BbrPI BcgI BciVI
BclI BfrI BglI BglII BlpI
BpmI Bpu1102I BsaBI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspEI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HinfI
HpaI HphI KpnI KspI MamI
MfeI MflI MluNI MroI MscI
MseI MslI MunI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp45I Tsp509I TspRI Tth111I XbaI
XhoII
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AflIII 497 ACGCGT A^C[GA][CT]GT
Asp700I 36 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 211 GAGCAC G[AT]GC[AT]^C
AvrII 123 CCTAGG C^CTAGG
BbsI 277 GTCTTC GAAGAC(2/6)
BbvCI 537 CCTCAGC CCTCAGC(-5/-2)
BfaI 124 CTAG C^TAG
BlnI 123 CCTAGG C^CTAGG
BpuAI 277 GTCTTC GAAGAC(2/6)
BsaAI 592 TACGTG [CT]AC^GT[GA]
BsiHKAI 211 GAGCAC G[AT]GC[AT]^C
BspDI 172 ATCGAT AT^CGAT
BspMI 254 ACCTGC ACCTGC(4/8)
BsrBI 310 CCGCTC CCGCTC(-3/-3)
BsrI 679 ACTGG ACTGG(1/-1)
BssHII 359 GCGCGC G^CGCGC
ClaI 172 ATCGAT AT^CGAT
DpnII 111 GATC ^GATC
DraII 337 GGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 268 GACAGGTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 332 CCGTGG C^C[GA][CT]GG
EarI 658 CTCTTC CTCTTC(1/4)
EcoO109I 337 GGGGCCT [GA]G^G[ACGT]CC[CT]
HgaI 499 GCGTC GACGC(5/10)
HindIII 139 AAGCTT A^AGCTT
Ksp632I 658 CTCTTC CTCTTC(1/4)
MaeI 124 CTAG C^TAG
MaeII 593 ACGT A^CGT
MaeIII 605 GTAAC ^GT[ACGT]AC
MboI 111 GATC ^GATC
MluI 497 ACGCGT A^CGCGT
NaeI 390 GCCGGC GCC^GGC
NdeII 111 GATC ^GATC
NgoMIV 390 GCCGGC G^CCGGC
PaeR7I 435 CTCGAG C^TCGAG
PstI 634 CTGCAG CTGCA^G
PvuII 540 CAGCTG CAG^CTG
Sau3AI 111 GATC ^GATC
SfaNI 96 GATGC GCATC(5/9)
SfcI 634 CTGCAG C^T[GA][CT]AG
XcmI 443 CCATCCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 435 CTCGAG C^TCGAG
XmnI 36 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 399 GGCGCC G[GA]^CG[CT]C
523 GGCGCC
AfaI 228 GTAC GT^AC
611 GTAC
BbeI 399 GGCGCC GGCGC^C
523 GGCGCC
BsaHI 399 GGCGCC G[GA]^CG[CT]C
523 GGCGCC
BsmFI 54 GTCCC GGGAC(10/14)
573 GTCCC
BsrFI 390 GCCGGC [GA]^CCGG[CT]
481 ACCGGC
BstF5I 444 CATCC GGATG(2/0)
654 CATCC
Cfr10I 390 GCCGGC [GA]^CCGG[CT]
481 ACCGGC
DdeI 355 CTGAG C^T[ACGT]AG
538 CTCAG
EaeI 163 CGGCCA [CT]^GGCC[GA]
713 CGGCCA
EcoT14I 123 CCTAGG C^C[AT][AT]GG
473 CCTTGG
FauI 309 CCCGC CCCGC(4/6)
421 CCCGC
FokI 444 CATCC GGATG(9/13)
654 CATCC
Hin1I 399 GGCGCC G[GA]^CG[CT]C
523 GGCGCC
KasI 399 GGCGCC G^GCGCC
523 GGCGCC
MspA1I 64 CAGCGG C[AC]G^C[GT]G
540 CAGCTG
NarI 399 GGCGCC GG^CGCC
523 GGCGCC
PflMI 447 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
468 CCACACCTTGG
RsaI 228 GTAC GT^AC
611 GTAC
SfoI 399 GGCGCC GGC^GCC
523 GGCGCC
SmaI 56 CCCGGG CCC^GGG
374 CCCGGG
StyI 123 CCTAGG C^C[AT][AT]GG
473 CCTTGG
TaqI 173 TCGA T^CGA
436 TCGA
TthHB8I 173 TCGA T^CGA
436 TCGA
Van91I 447 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
468 CCACACCTTGG
XmaCI 56 CCCGGG C^CCGGG
374 CCCGGG
XmaI 56 CCCGGG C^CCGGG
374 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AccII AciI AluI AvaI AvaII
BanI BbvI BcnI BmyI BsaJI
BsiYI BslI BsoBI Bsp1286I BssKI
BstNI BstUI Cac8I CfoI Cfr13I
EcoRII Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HpaII ItaI MboII
MnlI MspI MvaI MvnI MwoI
NciI NlaIII NlaIV Sau96I ScrFI
SmlI TseI