Restriction map for
pF1KB9646
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AvaI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BclI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaJI
BseRI BsgI BsiEI BsiWI BsmBI
BsoBI BspDI BspHI BspLU11I BspMI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MslI MunI NaeI NarI NciI
NcoI NgoMIV NheI NotI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI StyI SwaI
Tsp45I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccII 214 CGCG CG^CG
AccIII 83 TCCGGA T^CCGGA
AlwI 442 GATCC GGATC(4/5)
AlwNI 594 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 529 GAGCAC G[AT]GC[AT]^C
AspI 389 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 585 GGACC G^G[AT]CC
BalI 319 TGGCCA TGG^CCA
BcgI 404 CGAAGATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 283 GGATAC GTATCC(6/5)
BmyI 529 GAGCAC G[AGT]GC[ACT]^C
BsaI 209 GGTCTC GGTCTC(1/5)
BsaWI 83 TCCGGA [AT]^CCGG[AT]
BseAI 83 TCCGGA T^CCGGA
BsiHKAI 529 GAGCAC G[AT]GC[AT]^C
BsmAI 210 GTCTC GTCTC(1/5)
BsmFI 584 GGGAC GGGAC(10/14)
Bsp1286I 529 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 488 TGTACA T^GTACA
BspEI 83 TCCGGA T^CCGGA
BsrBI 77 CCGCTC CCGCTC(-3/-3)
BsrGI 488 TGTACA T^GTACA
BstF5I 495 GGATG GGATG(2/0)
BstUI 214 CGCG CG^CG
BstYI 441 AGATCC [GA]^GATC[CT]
CfoI 225 GCGC GCG^C
Cfr13I 585 GGACC G^G[ACGT]CC
EaeI 319 TGGCCA [CT]^GGCC[GA]
EarI 93 CTCTTC CTCTTC(1/4)
FokI 495 GGATG GGATG(9/13)
HapII 84 CCGG C^CGG
HhaI 225 GCGC GCG^C
HinP1I 225 GCGC G^CGC
HinfI 243 GAATC G^A[ACGT]TC
HpaII 84 CCGG C^CGG
HphI 571 TCACC GGTGA(8/7)
Ksp632I 93 CTCTTC CTCTTC(1/4)
MaeII 164 ACGT A^CGT
MaeIII 119 GTAAC ^GT[ACGT]AC
MflI 441 AGATCC [GA]^GATC[CT]
MluNI 319 TGGCCA TGG^CCA
MroI 83 TCCGGA T^CCGGA
MscI 319 TGGCCA TGG^CCA
MseI 25 TTAA T^TAA
MspI 84 CCGG C^CGG
MvnI 214 CGCG CG^CG
NdeI 8 CATATG CA^TATG
NlaIV 584 GGGACC GG[ACGT]^[ACGT]CC
NruI 213 TCGCGA TCG^CGA
PflFI 389 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 459 CTGCAG CTGCA^G
SapI 92 GCTCTTC GCTCTTC(1/4)
Sau96I 585 GGACC G^G[ACGT]CC
SfaNI 408 GATGC GCATC(5/9)
SmlI 519 CTCAAG C^T[CT][GA]AG
SspBI 488 TGTACA T^GTACA
SspI 70 AATATT AAT^ATT
TaqI 387 TCGA T^CGA
TfiI 243 GAATC G^A[AT]TC
Tru9I 25 TTAA T^TAA
Tth111I 389 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 387 TCGA T^CGA
XbaI 237 TCTAGA T^CTAGA
XhoII 441 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Asp700I 89 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
192 GAACGCATTC
BfaI 238 CTAG C^TAG
538 CTAG
BsiYI 77 CCGCTCTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
311 CCGAAAAATGG
BslI 77 CCGCTCTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
311 CCGAAAAATGG
BsmI 251 GAATGC GAATGC(1/-1)
196 GCATTC
BssKI 168 CCAGG ^CC[ACGT]GG
330 CCAGG
BstNI 168 CCAGG CC^[AT]GG
330 CCAGG
Cac8I 272 GCAAGC GC[ACGT]^[ACGT]GC
565 GCCAGC
DdeI 125 CTAAG C^T[ACGT]AG
261 CTCAG
EcoRII 168 CCAGG ^CC[AT]GG
330 CCAGG
MaeI 238 CTAG C^TAG
538 CTAG
MspA1I 56 CAGCTG C[AC]G^C[GT]G
277 CAGCTG
MvaI 168 CCAGG CC^[AT]GG
330 CCAGG
MwoI 107 GCACTGAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
439 GCAGATCCCGC
NlaIII 189 CATG CATG^
354 CATG
PvuII 56 CAGCTG CAG^CTG
277 CAGCTG
ScrFI 168 CCAGG CC^[ACGT]GG
330 CCAGG
SfcI 459 CTGCAG C^T[GA][CT]AG
591 CTACAG
XmnI 89 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
192 GAACGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AciI AfaI AluI BbvI BsrI
DpnII FauI Fnu4HI HaeIII ItaI
MboI MboII MnlI NdeII RsaI
Sau3AI TseI Tsp509I TspRI