Restriction map for
pF1KB9644
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BalI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BsaI BseAI
BsgI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I MamI MboII MfeI
MluI MluNI MroI MscI MseI
MunI NarI NcoI NdeI NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SalI SapI SbfI ScaI
SexAI SfcI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp45I Tsp509I Van91I XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AocI 253 CCTCAGG CC^T[ACGT]AGG
AspHI 81 GAGCTC G[AT]GC[AT]^C
AspI 302 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 524 TGCGCA TGC^GCA
BamHI 503 GGATCC G^GATCC
BanI 48 GGTGCC G^G[CT][GA]CC
BfaI 299 CTAG C^TAG
BlpI 83 GCTCAGC GC^T[ACGT]AGC
BpmI 214 CTGGAG CTGGAG(16/14)
Bpu1102I 83 GCTCAGC GC^T[ACGT]AGC
BsaWI 54 ACCGGA [AT]^CCGG[AT]
BseRI 111 CTCCTC GAGGAG(10/8)
BsiHKAI 81 GAGCTC G[AT]GC[AT]^C
BsmAI 313 GAGAC GTCTC(1/5)
BsmBI 313 GAGACG CGTCTC(1/5)
BssHII 271 GCGCGC G^CGCGC
BstYI 503 GGATCC [GA]^GATC[CT]
Bsu36I 253 CCTCAGG CC^T[ACGT]AGG
CelII 83 GCTCAGC GC^T[ACGT]AGC
EaeI 26 CGGCCA [CT]^GGCC[GA]
Eco81I 253 CCTCAGG CC^T[ACGT]AGG
EcoNI 477 CCTGCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 428 CCAAGG C^C[AT][AT]GG
FspI 524 TGCGCA TGC^GCA
HaeII 249 AGCGCC [GA]GCGC^[CT]
HphI 130 TCACC GGTGA(8/7)
MaeI 299 CTAG C^TAG
MaeII 316 ACGT A^CGT
MaeIII 98 GTAAC ^GT[ACGT]AC
MflI 503 GGATCC [GA]^GATC[CT]
MslI 516 CACGGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 292 GCCGGC GCC^GGC
NgoMIV 292 GCCGGC G^CCGGC
PflFI 302 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 360 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 81 GAGCTC GAGCT^C
SfiI 201 GGCCTCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 365 CCCGGG CCC^GGG
StuI 125 AGGCCT AGG^CCT
StyI 428 CCAAGG C^C[AT][AT]GG
TaqI 79 TCGA T^CGA
Tth111I 302 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 79 TCGA T^CGA
XbaI 298 TCTAGA T^CTAGA
XhoII 503 GGATCC [GA]^GATC[CT]
XmaCI 365 CCCGGG C^CCGGG
XmaI 365 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AlwI 504 GATCC GGATC(4/5)
503 GGATC
AlwNI 86 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
403 CAGTGGCTG
ApaI 229 GGGCCC GGGCC^C
494 GGGCCC
BsrFI 73 GCCGGT [GA]^CCGG[CT]
292 GCCGGC
Cfr10I 73 GCCGGT [GA]^CCGG[CT]
292 GCCGGC
DraII 285 AGGCCCC [GA]G^G[ACGT]CC[CT]
360 GGGTCCC
EcoO109I 285 AGGCCCC [GA]G^G[ACGT]CC[CT]
360 GGGTCCC
HinfI 36 GAGTC G^A[ACGT]TC
302 GACTC
PleI 302 GACTC GAGTC(4/5)
36 GAGTC
PvuII 382 CAGCTG CAG^CTG
463 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AluI AvaI AvaII
BanII BbvI BcnI BglI BmyI
BsaJI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI KspI MboI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIV SacII Sau3AI
Sau96I ScrFI SfaNI TseI TspRI