Restriction map for
pF1KB9633
##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BlnI BsaAI
BsaBI BsiEI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HhaI HinP1I HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MluNI MscI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SapI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccI 281 GTCGAC GT^[AC][GT]AC
AccIII 980 TCCGGA T^CCGGA
AciI 240 GCGG CCGC(-3/-1)
AcyI 39 GGCGTC G[GA]^CG[CT]C
AlwNI 1376 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 888 GGGCCC GGGCC^C
AvaI 813 CCCGAG C^[CT]CG[GA]G
BglII 265 AGATCT A^GATCT
BlpI 349 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 349 GCTGAGC GC^T[ACGT]AGC
BsaHI 39 GGCGTC G[GA]^CG[CT]C
BsaI 1172 GGTCTC GGTCTC(1/5)
BsaWI 980 TCCGGA [AT]^CCGG[AT]
BseAI 980 TCCGGA T^CCGGA
BseRI 127 GAGGAG GAGGAG(10/8)
BsgI 610 GTGCAG GTGCAG(16/14)
BsiYI 1110 CCCAAGTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1110 CCCAAGTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 41 CGTCTC CGTCTC(1/5)
BsmFI 880 GTCCC GGGAC(10/14)
BsoBI 813 CCCGAG C^[CT]CG[GA]G
BspEI 980 TCCGGA T^CCGGA
BsrDI 59 GCAATG GCAATG(2/0)
BssKI 294 CCTGG ^CC[ACGT]GG
BssSI 269 CTCGTG CACGAG(-5/-1)
BstBI 1087 TTCGAA TT^CGAA
BstF5I 894 CATCC GGATG(2/0)
BstNI 294 CCTGG CC^[AT]GG
BstYI 265 AGATCT [GA]^GATC[CT]
CelII 349 GCTGAGC GC^T[ACGT]AGC
DraI 342 TTTAAA TTT^AAA
EcoRII 294 CCTGG ^CC[AT]GG
EcoT14I 884 CCAAGG C^C[AT][AT]GG
FokI 894 CATCC GGATG(9/13)
HapII 981 CCGG C^CGG
Hin1I 39 GGCGTC G[GA]^CG[CT]C
HpaII 981 CCGG C^CGG
MaeII 820 ACGT A^CGT
MaeIII 1153 GTGAC ^GT[ACGT]AC
MflI 265 AGATCT [GA]^GATC[CT]
MroI 980 TCCGGA T^CCGGA
MslI 919 CATGTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 981 CCGG C^CGG
MvaI 294 CCTGG CC^[AT]GG
MwoI 969 GCCACACAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 398 CATATG CA^TATG
NspV 1087 TTCGAA TT^CGAA
PpuMI 878 AGGTCCC [GA]G^G[AT]CC[CT]
SacI 603 GAGCTC GAGCT^C
SalI 281 GTCGAC G^TCGAC
ScrFI 294 CCTGG CC^[ACGT]GG
SexAI 293 ACCTGGT A^CC[AT]GGT
SfuI 1087 TTCGAA TT^CGAA
SphI 924 GCATGC GCATG^C
StyI 884 CCAAGG C^C[AT][AT]GG
TfiI 411 GATTC G^A[AT]TC
Tsp45I 1153 GTGAC ^GT[GC]AC
XcmI 53 CCAGTAGCAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 265 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwI 797 GATCC GGATC(4/5)
983 GGATC
AvaII 78 GGTCC G^G[AT]CC
879 GGTCC
BanII 603 GAGCTC G[GA]GC[CT]^C
888 GGGCCC
BbsI 109 GAAGAC GAAGAC(2/6)
865 GAAGAC
BpuAI 109 GAAGAC GAAGAC(2/6)
865 GAAGAC
BsaJI 705 CCGAGG C^C[ACGT][ACGT]GG
884 CCAAGG
HaeIII 889 GGCC GG^CC
1038 GGCC
HincII 281 GTCGAC GT[CT]^[GA]AC
790 GTCAAC
HindII 281 GTCGAC GT[CT]^[GA]AC
790 GTCAAC
HphI 124 GGTGA GGTGA(8/7)
1057 TCACC
MspA1I 4 CAGCTG C[AC]G^C[GT]G
434 CAGCTG
PleI 112 GACTC GAGTC(4/5)
1378 GACTC
PstI 621 CTGCAG CTGCA^G
1322 CTGCAG
PvuII 4 CAGCTG CAG^CTG
434 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
AspHI BbvI BmyI BpmI BsiHKAI
BsmAI Bsp1286I BsrI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRI
Fnu4HI HgaI HinfI ItaI MboI
MboII MnlI MseI NdeII NlaIII
NlaIV NspI RsaI Sau3AI Sau96I
SfaNI SfcI SmlI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I XmnI