Restriction map for
pF1KB9631
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaII AviII AvrII
BalI BamHI BcgI BciVI BclI
BfaI BfrI BglII BlnI BpmI
BsaBI BsaWI BseAI BsiWI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DsaI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HinfI HpaI KpnI KspI MaeI
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp45I Tth111I Van91I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 338 GTAGAC GT^[AC][GT]AC
AfaI 247 GTAC GT^AC
AflIII 44 ACGTGT A^C[GA][CT]GT
AspHI 412 GTGCTC G[AT]GC[AT]^C
BanII 576 GAGCCC G[GA]GC[CT]^C
BbrPI 636 CACGTG CAC^GTG
BbsI 330 GAAGAC GAAGAC(2/6)
BbvCI 169 GCTGAGG CCTCAGC(-5/-2)
BlpI 573 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 573 GCTGAGC GC^T[ACGT]AGC
BpuAI 330 GAAGAC GAAGAC(2/6)
BsaAI 636 CACGTG [CT]AC^GT[GA]
BsiHKAI 412 GTGCTC G[AT]GC[AT]^C
BsrFI 221 GCCGGC [GA]^CCGG[CT]
BsrI 393 ACTGG ACTGG(1/-1)
BstF5I 101 CATCC GGATG(2/0)
CelII 573 GCTGAGC GC^T[ACGT]AGC
Cfr10I 221 GCCGGC [GA]^CCGG[CT]
DraII 536 GGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 90 GACCCCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 536 GGGGCCT [GA]G^G[ACGT]CC[CT]
FokI 101 CATCC GGATG(9/13)
MslI 775 CACCGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 221 GCCGGC GCC^GGC
NgoMIV 221 GCCGGC G^CCGGC
NspI 371 GCATGC [GA]CATG^[CT]
PmaCI 636 CACGTG CAC^GTG
PmlI 636 CACGTG CAC^GTG
PvuII 702 CAGCTG CAG^CTG
RsaI 247 GTAC GT^AC
SapI 552 GCTCTTC GCTCTTC(1/4)
SfaNI 323 GCATC GCATC(5/9)
SfcI 699 CTACAG C^T[GA][CT]AG
SphI 371 GCATGC GCATG^C
TaqI 452 TCGA T^CGA
Tsp509I 685 AATT ^AATT
TthHB8I 452 TCGA T^CGA
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AluI 440 AGCT AG^CT
703 AGCT
AlwI 61 GGATC GGATC(4/5)
513 GGATC
BsgI 705 CTGCAC GTGCAG(16/14)
316 GTGCAG
BsiEI 305 CGGCCG CG[GA][CT]^CG
728 CGGCCG
BsmBI 285 CGTCTC CGTCTC(1/5)
310 GAGACG
BstNI 507 CCTGG CC^[AT]GG
803 CCAGG
EaeI 305 CGGCCG [CT]^GGCC[GA]
728 CGGCCG
EagI 305 CGGCCG C^GGCCG
728 CGGCCG
EclXI 305 CGGCCG C^GGCCG
728 CGGCCG
Eco52I 305 CGGCCG C^GGCCG
728 CGGCCG
EcoRII 507 CCTGG ^CC[AT]GG
803 CCAGG
EcoT14I 20 CCTTGG C^C[AT][AT]GG
77 CCTTGG
MvaI 507 CCTGG CC^[AT]GG
803 CCAGG
NlaIII 372 CATG CATG^
741 CATG
SmaI 272 CCCGGG CCC^GGG
579 CCCGGG
StyI 20 CCTTGG C^C[AT][AT]GG
77 CCTTGG
XmaCI 272 CCCGGG C^CCGGG
579 CCCGGG
XmaI 272 CCCGGG C^CCGGG
579 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcyI AvaI BanI
BbeI BbvI BcnI BglI BmyI
BsaHI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsoBI Bsp1286I BssKI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII EarI FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI KasI
Ksp632I MaeII MboI MboII MnlI
MspA1I MspI MvnI MwoI NarI
NciI NdeII NlaIV Sau3AI Sau96I
ScrFI SfoI TseI TspRI