Restriction map for
pF1KB9629
##################################
# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AciI
AclI AcyI AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV FauI
FbaI FseI HaeII Hin1I KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MspA1I MunI MwoI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI SexAI
SfcI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XcmI
XhoI
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# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AcsI 443 AAATTC [GA]^AATT[CT]
AfaI 737 GTAC GT^AC
AlwNI 382 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 149 GGGCCC GGGCC^C
ApoI 443 AAATTC [GA]^AATT[CT]
Asp700I 56 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 643 GAGCTC G[AT]GC[AT]^C
AvaI 657 CCCGGG C^[CT]CG[GA]G
AviII 959 TGCGCA TGC^GCA
BalI 413 TGGCCA TGG^CCA
BanI 907 GGTGCC G^G[CT][GA]CC
BbsI 187 GAAGAC GAAGAC(2/6)
BglII 468 AGATCT A^GATCT
BpmI 155 CTGGAG CTGGAG(16/14)
BpuAI 187 GAAGAC GAAGAC(2/6)
BsaI 462 GAGACC GGTCTC(1/5)
BseRI 181 GAGGAG GAGGAG(10/8)
BsiHKAI 643 GAGCTC G[AT]GC[AT]^C
BsmI 70 GAATGC GAATGC(1/-1)
BsoBI 657 CCCGGG C^[CT]CG[GA]G
BsrI 411 ACTGG ACTGG(1/-1)
BssHII 301 GCGCGC G^CGCGC
BstEII 602 GGTCACC G^GT[ACGT]ACC
BstF5I 317 GGATG GGATG(2/0)
BstPI 602 GGTCACC G^GT[ACGT]ACC
BstYI 468 AGATCT [GA]^GATC[CT]
DpnII 469 GATC ^GATC
EaeI 413 TGGCCA [CT]^GGCC[GA]
EcoNI 897 CCTTGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 602 GGTCACC G^GT[ACGT]ACC
EcoT22I 865 ATGCAT ATGCA^T
FokI 317 GGATG GGATG(9/13)
FspI 959 TGCGCA TGC^GCA
HapII 658 CCGG C^CGG
HgaI 334 GACGC GACGC(5/10)
HincII 3 GTTAAC GT[CT]^[GA]AC
HindII 3 GTTAAC GT[CT]^[GA]AC
HindIII 105 AAGCTT A^AGCTT
HpaI 3 GTTAAC GTT^AAC
HpaII 658 CCGG C^CGG
MaeIII 603 GTCAC ^GT[ACGT]AC
MboI 469 GATC ^GATC
MflI 468 AGATCT [GA]^GATC[CT]
MluNI 413 TGGCCA TGG^CCA
MscI 413 TGGCCA TGG^CCA
MslI 868 CATTACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 658 CCGG C^CGG
NdeII 469 GATC ^GATC
NsiI 865 ATGCAT ATGCA^T
NspI 863 GCATGC [GA]CATG^[CT]
RsaI 737 GTAC GT^AC
SacI 643 GAGCTC GAGCT^C
Sau3AI 469 GATC ^GATC
ScaI 736 AGTACT AGT^ACT
SmaI 657 CCCGGG CCC^GGG
SphI 863 GCATGC GCATG^C
TaqI 129 TCGA T^CGA
Tsp45I 603 GTCAC ^GT[GC]AC
TthHB8I 129 TCGA T^CGA
XbaI 64 TCTAGA T^CTAGA
XhoII 468 AGATCT [GA]^GATC[CT]
XmaCI 657 CCCGGG C^CCGGG
XmaI 657 CCCGGG C^CCGGG
XmnI 56 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AflII 348 CTTAAG C^TTAAG
777 CTTAAG
BanII 149 GGGCCC G[GA]GC[CT]^C
643 GAGCTC
BcnI 657 CCCGG CC^[GC]GG
658 CCGGG
BfaI 65 CTAG C^TAG
221 CTAG
BfrI 348 CTTAAG C^TTAAG
777 CTTAAG
EarI 178 GAAGAG CTCTTC(1/4)
196 GAAGAG
EcoT14I 833 CCTTGG C^C[AT][AT]GG
897 CCTTGG
HphI 604 TCACC GGTGA(8/7)
647 TCACC
Ksp632I 178 GAAGAG CTCTTC(1/4)
196 GAAGAG
MaeI 65 CTAG C^TAG
221 CTAG
NciI 657 CCCGG CC^[GC]GG
658 CCGGG
PleI 669 GACTC GAGTC(4/5)
22 GAGTC
PpuMI 771 AGGTCCC [GA]G^G[AT]CC[CT]
891 GGGTCCC
SfaNI 318 GATGC GCATC(5/9)
140 GCATC
SmlI 348 CTTAAG C^T[CT][GA]AG
777 CTTAAG
StyI 833 CCTTGG C^C[AT][AT]GG
897 CCTTGG
TspRI 711 CAGTG CA[GC]TG[ACGT][ACGT]^
878 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AccII AluI AvaII BbvI BmyI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI DraII
EcoO109I EcoRII Fnu4HI HaeIII HhaI
HinP1I HinfI ItaI MboII MnlI
MseI MvaI MvnI NlaIII NlaIV
Sau96I ScrFI TfiI Tru9I TseI
Tsp509I