Restriction map for
pF1KB9600
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwNI
AocI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BamHI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BsaAI BsaBI
BsaWI BseAI BseRI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspMI BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FspI HincII HindII HindIII HinfI
HpaI KpnI MaeI MaeIII MamI
MfeI MluI MroI MseI MunI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI SbfI ScaI SexAI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp45I Tsp509I Tth111I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 631 GTCTAC GT^[AC][GT]AC
AfaI 861 GTAC GT^AC
AflIII 404 ACATGT A^C[GA][CT]GT
AlwI 339 GATCC GGATC(4/5)
BalI 191 TGGCCA TGG^CCA
BbsI 667 GTCTTC GAAGAC(2/6)
BlpI 693 GCTCAGC GC^T[ACGT]AGC
BpmI 626 CTCCAG CTGGAG(16/14)
Bpu1102I 693 GCTCAGC GC^T[ACGT]AGC
BpuAI 667 GTCTTC GAAGAC(2/6)
BsaI 955 GAGACC GGTCTC(1/5)
BsmBI 70 GAGACG CGTCTC(1/5)
BspLU11I 404 ACATGT A^CATGT
BssHII 562 GCGCGC G^CGCGC
BstF5I 300 CATCC GGATG(2/0)
BstYI 338 AGATCC [GA]^GATC[CT]
CelII 693 GCTCAGC GC^T[ACGT]AGC
EarI 58 GAAGAG CTCTTC(1/4)
EcoT14I 188 CCATGG C^C[AT][AT]GG
FokI 300 CATCC GGATG(9/13)
HphI 248 TCACC GGTGA(8/7)
Ksp632I 58 GAAGAG CTCTTC(1/4)
MaeII 982 ACGT A^CGT
MflI 338 AGATCC [GA]^GATC[CT]
MluNI 191 TGGCCA TGG^CCA
MscI 191 TGGCCA TGG^CCA
MslI 183 CATCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 188 CCATGG C^CATGG
NotI 687 GCGGCCGC GC^GGCCGC
PflMI 281 CCAAAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 145 CTGCAG CTGCA^G
RsaI 861 GTAC GT^AC
SapI 58 GAAGAGC GCTCTTC(1/4)
SfiI 804 GGCCCGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 753 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 334 CTCAAG C^T[CT][GA]AG
StyI 188 CCATGG C^C[AT][AT]GG
Van91I 281 CCAAAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 338 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BsgI 795 CTGCAC GTGCAG(16/14)
1049 GTGCAG
BsmAI 70 GAGAC GTCTC(1/5)
955 GAGAC
BsmFI 912 GGGAC GGGAC(10/14)
91 GTCCC
DdeI 683 CTGAG C^T[ACGT]AG
694 CTCAG
FseI 351 GGCCGGCC GGCCGG^CC
809 GGCCGGCC
KspI 111 CCGCGG CCGC^GG
641 CCGCGG
NspI 404 ACATGT [GA]CATG^[CT]
479 ACATGC
PpuMI 89 GGGTCCC [GA]G^G[AT]CC[CT]
912 GGGACCC
SacII 111 CCGCGG CCGC^GG
641 CCGCGG
SfaNI 233 GCATC GCATC(5/9)
299 GCATC
SfcI 145 CTGCAG C^T[GA][CT]AG
165 CTACAG
SmaI 360 CCCGGG CCC^GGG
576 CCCGGG
TaqI 386 TCGA T^CGA
410 TCGA
TthHB8I 386 TCGA T^CGA
410 TCGA
XmaCI 360 CCCGGG C^CCGGG
576 CCCGGG
XmaI 360 CCCGGG C^CCGGG
576 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcyI AluI Aor51HI
ApaI AvaI AvaII BanI BanII
BbeI BbvI BcnI BglI BmyI
BsaHI BsaJI BsiEI BsiYI BslI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DpnII DraII DsaI
EaeI EagI EclXI Eco47III Eco52I
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII ItaI KasI MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV Sau3AI
Sau96I ScrFI SfoI TseI TspRI