Restriction map for
pF1KB9587
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AclI AcsI AfaI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbsI BcgI BciVI BclI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BsaI BsgI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraI DrdI Eam1105I
EarI Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI HphI KasI KpnI Ksp632I
MaeII MaeIII MamI MboII MfeI
MluI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp45I Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 249 CGCG CG^CG
AccIII 621 TCCGGA T^CCGGA
AcyI 234 GGCGTC G[GA]^CG[CT]C
AlwI 79 GATCC GGATC(4/5)
AocI 592 CCTGAGG CC^T[ACGT]AGG
ApaI 474 GGGCCC GGGCC^C
AspHI 148 GTGCTC G[AT]GC[AT]^C
BanII 474 GGGCCC G[GA]GC[CT]^C
BbvCI 363 CCTCAGC CCTCAGC(-5/-2)
BglI 337 GCCTCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3 GCTGAGC GC^T[ACGT]AGC
BsaHI 234 GGCGTC G[GA]^CG[CT]C
BsaWI 621 TCCGGA [AT]^CCGG[AT]
BseAI 621 TCCGGA T^CCGGA
BsiHKAI 148 GTGCTC G[AT]GC[AT]^C
BspEI 621 TCCGGA T^CCGGA
BstAPI 195 GCACCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 249 CGCG CG^CG
BstXI 347 CCAGCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 78 AGATCC [GA]^GATC[CT]
Bsu36I 592 CCTGAGG CC^T[ACGT]AGG
CelII 3 GCTGAGC GC^T[ACGT]AGC
DpnII 79 GATC ^GATC
DraIII 196 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 113 CGGCCG C^GGCCG
EclXI 113 CGGCCG C^GGCCG
Eco52I 113 CGGCCG C^GGCCG
Eco81I 592 CCTGAGG CC^T[ACGT]AGG
FauI 247 CCCGC CCCGC(4/6)
HaeII 444 AGCGCC [GA]GCGC^[CT]
Hin1I 234 GGCGTC G[GA]^CG[CT]C
HincII 61 GTCGAC GT[CT]^[GA]AC
HindII 61 GTCGAC GT[CT]^[GA]AC
KspI 248 CCGCGG CCGC^GG
MboI 79 GATC ^GATC
MflI 78 AGATCC [GA]^GATC[CT]
MroI 621 TCCGGA T^CCGGA
MvnI 249 CGCG CG^CG
NdeII 79 GATC ^GATC
PpuMI 359 AGGACCT [GA]G^G[AT]CC[CT]
SacII 248 CCGCGG CCGC^GG
SalI 61 GTCGAC G^TCGAC
Sau3AI 79 GATC ^GATC
SexAI 197 ACCTGGT A^CC[AT]GGT
TaqI 62 TCGA T^CGA
Tsp509I 641 AATT ^AATT
TthHB8I 62 TCGA T^CGA
XhoII 78 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 61 GTCGAC GT^[AC][GT]AC
491 GTCTAC
BalI 84 TGGCCA TGG^CCA
344 TGGCCA
BpmI 355 CTGGAG CTGGAG(16/14)
682 CTGGAG
BsiEI 113 CGGCCG CG[GA][CT]^CG
688 CGACCG
HgaI 322 GACGC GACGC(5/10)
235 GCGTC
HinfI 64 GACTC G^A[ACGT]TC
384 GAGTC
MluNI 84 TGGCCA TGG^CCA
344 TGGCCA
MscI 84 TGGCCA TGG^CCA
344 TGGCCA
MspA1I 92 CAGCGG C[AC]G^C[GT]G
248 CCGCGG
NcoI 239 CCATGG C^CATGG
341 CCATGG
PflMI 396 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
478 CCAGCCACTGG
PleI 64 GACTC GAGTC(4/5)
384 GAGTC
PstI 169 CTGCAG CTGCA^G
281 CTGCAG
SfaNI 227 GCATC GCATC(5/9)
431 GCATC
SfcI 169 CTGCAG C^T[GA][CT]AG
281 CTGCAG
SmlI 151 CTCAAG C^T[CT][GA]AG
508 CTTGAG
Van91I 396 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
478 CCAGCCACTGG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanI
BbvI BcnI BfaI BmyI BsaJI
BseRI BsiYI BslI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EaeI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII ItaI MaeI MnlI
MslI MspI MvaI MwoI NciI
NlaIII NlaIV Sau96I ScrFI StyI
TseI TspRI