Restriction map for
pF1KB8799
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# NO CUT ENZYMES ( 188 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfrI
BglI BlnI BlpI BmyI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV EcoT14I EcoT22I FauI FseI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII KasI
KpnI KspI MamI MfeI MluI
MluNI MnlI MroI MscI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AlwNI 747 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
BamHI 924 GGATCC G^GATCC
BbvI 947 GCTGC GCAGC(8/12)
BclI 89 TGATCA T^GATCA
BglII 703 AGATCT A^GATCT
BpmI 687 CTCCAG CTGGAG(16/14)
BsaI 467 GAGACC GGTCTC(1/5)
BsaJI 832 CCCTGG C^C[ACGT][ACGT]GG
BsmAI 467 GAGAC GTCTC(1/5)
BsrDI 861 CATTGC GCAATG(2/0)
BstAPI 496 GCACAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 755 GGTTACC G^GT[ACGT]ACC
BstF5I 680 CATCC GGATG(2/0)
BstPI 755 GGTTACC G^GT[ACGT]ACC
DdeI 745 CTCAG C^T[ACGT]AG
DraI 900 TTTAAA TTT^AAA
EarI 12 CTCTTC CTCTTC(1/4)
EcoO65I 755 GGTTACC G^GT[ACGT]ACC
FbaI 89 TGATCA T^GATCA
Fnu4HI 947 GCTGC GC^[ACGT]GC
FokI 680 CATCC GGATG(9/13)
HinfI 804 GATTC G^A[ACGT]TC
HpaI 70 GTTAAC GTT^AAC
HphI 567 GGTGA GGTGA(8/7)
ItaI 947 GCTGC GC^[ACGT]GC
Ksp632I 12 CTCTTC CTCTTC(1/4)
MaeII 45 ACGT A^CGT
MwoI 496 GCACAGAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 564 CATG CATG^
NlaIV 924 GGATCC GG[ACGT]^[ACGT]CC
SexAI 751 ACCTGGT A^CC[AT]GGT
SfcI 866 CTACAG C^T[GA][CT]AG
SmlI 976 CTTGAG C^T[CT][GA]AG
TfiI 804 GATTC G^A[AT]TC
TseI 947 GCTGC G^C[AT]GC
XcmI 928 CCATTTGTCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 156 AAATTT [GA]^AATT[CT]
366 GAATTT
AlwI 925 GATCC GGATC(4/5)
924 GGATC
ApoI 156 AAATTT [GA]^AATT[CT]
366 GAATTT
BssKI 752 CCTGG ^CC[ACGT]GG
833 CCTGG
BstNI 752 CCTGG CC^[AT]GG
833 CCTGG
BstYI 703 AGATCT [GA]^GATC[CT]
924 GGATCC
EcoRII 752 CCTGG ^CC[AT]GG
833 CCTGG
HincII 70 GTTAAC GT[CT]^[GA]AC
934 GTCAAC
HindII 70 GTTAAC GT[CT]^[GA]AC
934 GTCAAC
HindIII 385 AAGCTT A^AGCTT
892 AAGCTT
MflI 703 AGATCT [GA]^GATC[CT]
924 GGATCC
MslI 392 CATTTAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
564 CATGGTGATG
MvaI 752 CCTGG CC^[AT]GG
833 CCTGG
ScrFI 752 CCTGG CC^[ACGT]GG
833 CCTGG
Tsp45I 515 GTCAC ^GT[GC]AC
887 GTCAC
TspRI 517 CACTG CA[GC]TG[ACGT][ACGT]^
869 CAGTG
XhoII 703 AGATCT [GA]^GATC[CT]
924 GGATCC
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# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AfaI AluI BfaI BsmI BsrI
DpnII MaeI MaeIII MboI MboII
MseI NdeII RsaI Sau3AI SspI
Tru9I Tsp509I