Restriction map for
pF1KB8671
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# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbsI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoT22I FauI FbaI FokI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaII KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 676 GTCTAC GT^[AC][GT]AC
AflIII 370 ACACGT A^C[GA][CT]GT
AlwNI 310 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 982 CCTCAGG CC^T[ACGT]AGG
ApaI 481 GGGCCC GGGCC^C
Asp700I 448 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 296 GAGCTC G[AT]GC[AT]^C
AvaII 956 GGACC G^G[AT]CC
BbrPI 371 CACGTG CAC^GTG
BbvCI 826 GCTGAGG CCTCAGC(-5/-2)
BsaAI 371 CACGTG [CT]AC^GT[GA]
BsgI 162 CTGCAC GTGCAG(16/14)
BsiHKAI 296 GAGCTC G[AT]GC[AT]^C
BsmAI 834 GAGAC GTCTC(1/5)
BsmFI 970 GGGAC GGGAC(10/14)
BsmI 321 GAATGC GAATGC(1/-1)
BspHI 609 TCATGA T^CATGA
BstYI 417 AGATCC [GA]^GATC[CT]
Bsu36I 982 CCTCAGG CC^T[ACGT]AGG
DraI 1057 TTTAAA TTT^AAA
DraIII 863 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 448 GAAGAG CTCTTC(1/4)
Eco81I 982 CCTCAGG CC^T[ACGT]AGG
EcoRV 391 GATATC GAT^ATC
EcoT14I 797 CCTTGG C^C[AT][AT]GG
HincII 123 GTTAAC GT[CT]^[GA]AC
HindII 123 GTTAAC GT[CT]^[GA]AC
HpaI 123 GTTAAC GTT^AAC
HphI 19 GGTGA GGTGA(8/7)
Ksp632I 448 GAAGAG CTCTTC(1/4)
MaeIII 761 GTGAC ^GT[ACGT]AC
MflI 417 AGATCC [GA]^GATC[CT]
MslI 337 CATATTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PmaCI 371 CACGTG CAC^GTG
PmlI 371 CACGTG CAC^GTG
RcaI 609 TCATGA T^CATGA
SacI 296 GAGCTC GAGCT^C
SapI 448 GAAGAGC GCTCTTC(1/4)
SfaNI 356 GCATC GCATC(5/9)
SmlI 805 CTCAAG C^T[CT][GA]AG
StyI 797 CCTTGG C^C[AT][AT]GG
TaqI 426 TCGA T^CGA
Tsp45I 761 GTGAC ^GT[GC]AC
TthHB8I 426 TCGA T^CGA
XhoII 417 AGATCC [GA]^GATC[CT]
XmnI 448 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 248 AAATTC [GA]^AATT[CT]
1156 AAATTT
AfaI 190 GTAC GT^AC
1101 GTAC
AlwI 172 GATCC GGATC(4/5)
418 GATCC
ApoI 248 AAATTC [GA]^AATT[CT]
1156 AAATTT
BanII 296 GAGCTC G[GA]GC[CT]^C
481 GGGCCC
BfaI 499 CTAG C^TAG
913 CTAG
BmyI 296 GAGCTC G[AGT]GC[ACT]^C
481 GGGCCC
Bsp1286I 296 GAGCTC G[AGT]GC[ACT]^C
481 GGGCCC
DraII 311 AGGGCCT [GA]G^G[ACGT]CC[CT]
793 GGGGCCT
EcoO109I 311 AGGGCCT [GA]G^G[ACGT]CC[CT]
793 GGGGCCT
HinfI 999 GATTC G^A[ACGT]TC
1022 GATTC
MaeI 499 CTAG C^TAG
913 CTAG
MaeII 372 ACGT A^CGT
401 ACGT
PflMI 40 CCAATCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
91 CCAAATGTTGG
PstI 929 CTGCAG CTGCA^G
989 CTGCAG
RsaI 190 GTAC GT^AC
1101 GTAC
SfcI 929 CTGCAG C^T[GA][CT]AG
989 CTGCAG
TfiI 999 GATTC G^A[AT]TC
1022 GATTC
TspRI 65 CACTG CA[GC]TG[ACGT][ACGT]^
927 CACTG
Van91I 40 CCAATCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
91 CCAAATGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AluI BbvI BsaJI BseRI BsiYI
BslI BssKI BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI HaeIII
ItaI MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI Tru9I TseI
Tsp509I