Restriction map for
pF1KB8669
##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I AspEI AspI BbrPI BbvCI
BcgI BfrI BsaBI BsaWI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
HincII HindII HindIII HpaI KspI
MamI MfeI MluI MroI MunI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
ScaI SfuI SgrAI SmlI SnaBI
SpeI SphI SspBI SspI SwaI
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Alw44I 1063 GTGCAC G^TGCAC
ApaLI 1063 GTGCAC G^TGCAC
AviII 287 TGCGCA TGC^GCA
AvrII 297 CCTAGG C^CTAGG
BamHI 956 GGATCC G^GATCC
BbeI 1337 GGCGCC GGCGC^C
BciVI 498 GTATCC GTATCC(6/5)
BclI 1424 TGATCA T^GATCA
BlnI 297 CCTAGG C^CTAGG
BsaAI 1408 TACGTG [CT]AC^GT[GA]
BsaI 48 GGTCTC GGTCTC(1/5)
BseRI 739 GAGGAG GAGGAG(10/8)
BsgI 1144 CTGCAC GTGCAG(16/14)
BsmBI 1096 GAGACG CGTCTC(1/5)
BstXI 825 CCAGGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 893 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1006 GACCCCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 214 CTCTTC CTCTTC(1/4)
FbaI 1424 TGATCA T^GATCA
FspI 287 TGCGCA TGC^GCA
KasI 1337 GGCGCC G^GCGCC
Ksp632I 214 CTCTTC CTCTTC(1/4)
MseI 809 TTAA T^TAA
NarI 1337 GGCGCC GG^CGCC
PvuII 943 CAGCTG CAG^CTG
SacI 1300 GAGCTC GAGCT^C
SbfI 960 CCTGCAGG CCTGCA^GG
SexAI 670 ACCAGGT A^CC[AT]GGT
SfoI 1337 GGCGCC GGC^GCC
SmaI 131 CCCGGG CCC^GGG
Sse8387I 960 CCTGCAGG CCTGCA^GG
TaqI 1511 TCGA T^CGA
TfiI 116 GAATC G^A[AT]TC
Tru9I 809 TTAA T^TAA
Tsp509I 1106 AATT ^AATT
TthHB8I 1511 TCGA T^CGA
XcmI 345 CCACCTGGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 131 CCCGGG C^CCGGG
XmaI 131 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 674 GGTACC G^GTACC
923 GGTACC
AcyI 1337 GGCGCC G[GA]^CG[CT]C
1439 GGCGTC
AlwI 957 GATCC GGATC(4/5)
956 GGATC
ApaI 861 GGGCCC GGGCC^C
1611 GGGCCC
Asp718I 674 GGTACC G^GTACC
923 GGTACC
AvaI 131 CCCGGG C^[CT]CG[GA]G
1522 CCCGAG
BalI 366 TGGCCA TGG^CCA
1661 TGGCCA
BbsI 1071 GAAGAC GAAGAC(2/6)
1087 GTCTTC
BfaI 298 CTAG C^TAG
1699 CTAG
BglII 1019 AGATCT A^GATCT
1290 AGATCT
BlpI 427 GCTGAGC GC^T[ACGT]AGC
1545 GCTCAGC
Bpu1102I 427 GCTGAGC GC^T[ACGT]AGC
1545 GCTCAGC
BpuAI 1071 GAAGAC GAAGAC(2/6)
1087 GTCTTC
BsaHI 1337 GGCGCC G[GA]^CG[CT]C
1439 GGCGTC
BsiEI 1331 CGGCCG CG[GA][CT]^CG
1635 CGGCCG
BsoBI 131 CCCGGG C^[CT]CG[GA]G
1522 CCCGAG
BsrBI 1249 CCGCTC CCGCTC(-3/-3)
852 GAGCGG
BsrFI 29 GCCGGC [GA]^CCGG[CT]
845 GCCGGC
CelII 427 GCTGAGC GC^T[ACGT]AGC
1545 GCTCAGC
Cfr10I 29 GCCGGC [GA]^CCGG[CT]
845 GCCGGC
EagI 1331 CGGCCG C^GGCCG
1635 CGGCCG
EclXI 1331 CGGCCG C^GGCCG
1635 CGGCCG
Eco52I 1331 CGGCCG C^GGCCG
1635 CGGCCG
Hin1I 1337 GGCGCC G[GA]^CG[CT]C
1439 GGCGTC
KpnI 674 GGTACC GGTAC^C
923 GGTACC
MaeI 298 CTAG C^TAG
1699 CTAG
MluNI 366 TGGCCA TGG^CCA
1661 TGGCCA
MscI 366 TGGCCA TGG^CCA
1661 TGGCCA
MspA1I 527 CCGCTG C[AC]G^C[GT]G
943 CAGCTG
NaeI 29 GCCGGC GCC^GGC
845 GCCGGC
NgoMIV 29 GCCGGC G^CCGGC
845 GCCGGC
PflMI 865 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
942 CCAGCTGGTGG
PstI 509 CTGCAG CTGCA^G
961 CTGCAG
SfcI 509 CTGCAG C^T[GA][CT]AG
961 CTGCAG
StuI 321 AGGCCT AGG^CCT
1415 AGGCCT
Van91I 865 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
942 CCAGCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaII BanI BanII BbvI
BcnI BglI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfiI StyI
TseI Tsp45I TspRI XhoII