Restriction map for
pF1KB8630
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AhdI Alw44I
AocI ApaLI AscI AseI AsnI
Asp718I AspEI BbeI BbrPI BcgI
BclI BfrI BglI BglII BlpI
Bpu1102I BsaAI BsaBI BsaHI BseAI
BsiEI BsiWI BspDI BspEI BspLU11I
BsrBI BssHII BssSI BstAPI BstBI
BstUI Bsu36I CelII ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoRI EcoRV FbaI
FseI Hin1I HincII HindII HpaI
KasI KpnI KspI MaeII MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI TaqI TthHB8I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AcsI 1523 AAATTT [GA]^AATT[CT]
AgeI 1699 ACCGGT A^CCGGT
Aor51HI 22 AGCGCT AGC^GCT
ApaI 2411 GGGCCC GGGCC^C
ApoI 1523 AAATTT [GA]^AATT[CT]
Asp700I 1891 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1806 GAGCTC G[AT]GC[AT]^C
AvaI 990 CCCGAG C^[CT]CG[GA]G
AviII 532 TGCGCA TGC^GCA
AvrII 1833 CCTAGG C^CTAGG
BalI 1655 TGGCCA TGG^CCA
BamHI 2118 GGATCC G^GATCC
BanI 718 GGTGCC G^G[CT][GA]CC
BbsI 2242 GTCTTC GAAGAC(2/6)
BbvCI 2319 GCTGAGG CCTCAGC(-5/-2)
BciVI 337 GGATAC GTATCC(6/5)
BcnI 722 CCCGG CC^[GC]GG
BlnI 1833 CCTAGG C^CTAGG
BpuAI 2242 GTCTTC GAAGAC(2/6)
BsaI 298 GGTCTC GGTCTC(1/5)
BsaWI 1699 ACCGGT [AT]^CCGG[AT]
BsiHKAI 1806 GAGCTC G[AT]GC[AT]^C
BsmBI 2176 CGTCTC CGTCTC(1/5)
BsoBI 990 CCCGAG C^[CT]CG[GA]G
BspHI 1848 TCATGA T^CATGA
BsrDI 1010 GCAATG GCAATG(2/0)
Bst1107I 1882 GTATAC GTA^TAC
BstEII 1489 GGTAACC G^GT[ACGT]ACC
BstPI 1489 GGTAACC G^GT[ACGT]ACC
BstXI 228 CCATGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1882 GTATAC GTA^TAC
DraI 1692 TTTAAA TTT^AAA
DraIII 934 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 124 CCATGG C^C[GA][CT]GG
Eco47III 22 AGCGCT AGC^GCT
EcoO65I 1489 GGTAACC G^GT[ACGT]ACC
EcoT22I 2143 ATGCAT ATGCA^T
FspI 532 TGCGCA TGC^GCA
HgaI 129 GACGC GACGC(5/10)
MluNI 1655 TGGCCA TGG^CCA
MscI 1655 TGGCCA TGG^CCA
MslI 2368 CACCTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 722 CCCGG CC^[GC]GG
NcoI 124 CCATGG C^CATGG
NsiI 2143 ATGCAT ATGCA^T
PflMI 497 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1699 ACCGGT A^CCGGT
PstI 2416 CTGCAG CTGCA^G
RcaI 1848 TCATGA T^CATGA
SacI 1806 GAGCTC GAGCT^C
SphI 282 GCATGC GCATG^C
SwaI 1691 ATTTAAAT ATTT^AAAT
Van91I 497 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1891 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 997 GTAGAC GT^[AC][GT]AC
1882 GTATAC
AlwNI 290 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
498 CAGAACCTG
AspI 1092 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2431 GACGATGTC
AvaII 105 GGACC G^G[AT]CC
1788 GGACC
BseRI 445 CTCCTC GAGGAG(10/8)
796 CTCCTC
BsgI 204 CTGCAC GTGCAG(16/14)
2365 CTGCAC
BsmI 1006 GAATGC GAATGC(1/-1)
1041 GAATGC
Bsp1407I 1826 TGTACA T^GTACA
1934 TGTACA
BspMI 2405 ACCTGC ACCTGC(4/8)
1622 GCAGGT
BsrFI 194 ACCGGC [GA]^CCGG[CT]
1699 ACCGGT
BsrGI 1826 TGTACA T^GTACA
1934 TGTACA
Cfr10I 194 ACCGGC [GA]^CCGG[CT]
1699 ACCGGT
EaeI 196 CGGCCA [CT]^GGCC[GA]
1655 TGGCCA
HaeII 22 AGCGCT [GA]GCGC^[CT]
1540 AGCGCC
HindIII 309 AAGCTT A^AGCTT
753 AAGCTT
NheI 2058 GCTAGC G^CTAGC
2272 GCTAGC
PflFI 1092 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2431 GACGATGTC
PpuMI 104 AGGACCT [GA]G^G[AT]CC[CT]
1787 AGGACCC
SapI 984 GAAGAGC GCTCTTC(1/4)
2094 GCTCTTC
SspBI 1826 TGTACA T^GTACA
1934 TGTACA
StuI 1669 AGGCCT AGG^CCT
2323 AGGCCT
Tth111I 1092 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2431 GACGATGTC
XcmI 115 CCAGAAACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1474 CCAGCTAAAGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AlwI BanII
BbvI BfaI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII