Restriction map for
pF1KB8602
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbsI BcgI BclI BcnI BfaI
BfrI BlnI BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HapII Hin1I HincII
HindII HpaI HpaII KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MseI MspI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI Tru9I Tsp509I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 609 CGCG CG^CG
ApaI 604 GGGCCC GGGCC^C
Asp700I 123 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 225 TGGCCA TGG^CCA
BanII 604 GGGCCC G[GA]GC[CT]^C
BbvCI 405 CCTCAGC CCTCAGC(-5/-2)
BciVI 137 GTATCC GTATCC(6/5)
BglI 606 GCCCGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 209 AGATCT A^GATCT
BlpI 325 GCTCAGC GC^T[ACGT]AGC
BmyI 604 GGGCCC G[AGT]GC[ACT]^C
BpmI 346 CTGGAG CTGGAG(16/14)
Bpu1102I 325 GCTCAGC GC^T[ACGT]AGC
BsaAI 115 TACGTG [CT]AC^GT[GA]
BsgI 358 CTGCAC GTGCAG(16/14)
BsmFI 215 GGGAC GGGAC(10/14)
Bsp1286I 604 GGGCCC G[AGT]GC[ACT]^C
BsrBI 415 GAGCGG CCGCTC(-3/-3)
BstEII 423 GGTGACC G^GT[ACGT]ACC
BstPI 423 GGTGACC G^GT[ACGT]ACC
BstUI 609 CGCG CG^CG
BstYI 209 AGATCT [GA]^GATC[CT]
CelII 325 GCTCAGC GC^T[ACGT]AGC
DpnII 210 GATC ^GATC
DraIII 499 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 86 CCGTGG C^C[GA][CT]GG
EarI 107 CTCTTC CTCTTC(1/4)
EcoO65I 423 GGTGACC G^GT[ACGT]ACC
HgaI 245 GCGTC GACGC(5/10)
HindIII 202 AAGCTT A^AGCTT
HinfI 310 GATTC G^A[ACGT]TC
Ksp632I 107 CTCTTC CTCTTC(1/4)
MaeII 116 ACGT A^CGT
MaeIII 424 GTGAC ^GT[ACGT]AC
MboI 210 GATC ^GATC
MflI 209 AGATCT [GA]^GATC[CT]
MluNI 225 TGGCCA TGG^CCA
MscI 225 TGGCCA TGG^CCA
MslI 168 CACAAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 609 CGCG CG^CG
NdeII 210 GATC ^GATC
NlaIII 474 CATG CATG^
PstI 541 CTGCAG CTGCA^G
SapI 106 GCTCTTC GCTCTTC(1/4)
Sau3AI 210 GATC ^GATC
SexAI 500 ACCAGGT A^CC[AT]GGT
SfcI 541 CTGCAG C^T[GA][CT]AG
TfiI 310 GATTC G^A[AT]TC
Tsp45I 424 GTGAC ^GT[GC]AC
XhoII 209 AGATCT [GA]^GATC[CT]
XmnI 123 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AciI 608 CCGC CCGC(-3/-1)
417 GCGG
AlwNI 243 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
331 CAGTGGCTG
BanI 187 GGTGCC G^G[CT][GA]CC
268 GGTGCC
BstF5I 180 GGATG GGATG(2/0)
579 GGATG
CfoI 280 GCGC GCG^C
629 GCGC
DdeI 326 CTCAG C^T[ACGT]AG
406 CTCAG
EaeI 225 TGGCCA [CT]^GGCC[GA]
485 CGGCCA
EcoNI 345 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
405 CCTCAGCCAGG
FauI 607 CCCGC CCCGC(4/6)
417 GCGGG
FokI 180 GGATG GGATG(9/13)
579 GGATG
HhaI 280 GCGC GCG^C
629 GCGC
HinP1I 280 GCGC G^CGC
629 GCGC
MspA1I 221 CAGCTG C[AC]G^C[GT]G
584 CAGCTG
PpuMI 16 AGGACCC [GA]G^G[AT]CC[CT]
341 AGGACCT
PvuII 221 CAGCTG CAG^CTG
584 CAGCTG
SfaNI 381 GATGC GCATC(5/9)
580 GATGC
SmlI 205 CTTGAG C^T[CT][GA]AG
319 CTCAAG
TspRI 331 CAGTG CA[GC]TG[ACGT][ACGT]^
524 CAGTG
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AfaI AluI AvaII BbvI BsaJI
BseRI BsiYI BslI BssKI BstNI
Cac8I Cfr13I DraII EcoO109I EcoRII
Fnu4HI HaeIII HphI ItaI MboII
MnlI MvaI MwoI NlaIV RsaI
Sau96I ScrFI TseI