Restriction map for
pF1KB8586
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmFI BsmI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FauI FbaI FokI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MroI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TspRI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 766 CGCG CG^CG
AfaI 453 GTAC GT^AC
AflIII 765 ACGCGT A^C[GA][CT]GT
AlwNI 896 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 313 CCCGGG C^[CT]CG[GA]G
AvaII 858 GGACC G^G[AT]CC
BbsI 447 GAAGAC GAAGAC(2/6)
BfaI 688 CTAG C^TAG
BglII 354 AGATCT A^GATCT
BmyI 463 GTGCCC G[AGT]GC[ACT]^C
BpmI 595 CTGGAG CTGGAG(16/14)
BpuAI 447 GAAGAC GAAGAC(2/6)
BsmAI 580 GTCTC GTCTC(1/5)
BsmBI 579 CGTCTC CGTCTC(1/5)
BsoBI 313 CCCGGG C^[CT]CG[GA]G
Bsp1286I 463 GTGCCC G[AGT]GC[ACT]^C
BspHI 692 TCATGA T^CATGA
BsrI 165 ACTGG ACTGG(1/-1)
BstUI 766 CGCG CG^CG
Cac8I 741 GCCAGC GC[ACGT]^[ACGT]GC
EcoRI 469 GAATTC G^AATTC
HapII 314 CCGG C^CGG
HgaI 764 GACGC GACGC(5/10)
HpaII 314 CCGG C^CGG
HphI 100 GGTGA GGTGA(8/7)
MaeI 688 CTAG C^TAG
MluI 765 ACGCGT A^CGCGT
MseI 290 TTAA T^TAA
MslI 780 CACAATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 743 CAGCTG C[AC]G^C[GT]G
MspI 314 CCGG C^CGG
MvnI 766 CGCG CG^CG
MwoI 801 GCTCCATATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 805 CATATG CA^TATG
PleI 178 GACTC GAGTC(4/5)
PstI 902 CTGCAG CTGCA^G
PvuII 743 CAGCTG CAG^CTG
RcaI 692 TCATGA T^CATGA
RsaI 453 GTAC GT^AC
SexAI 272 ACCAGGT A^CC[AT]GGT
SfcI 902 CTGCAG C^T[GA][CT]AG
SmaI 313 CCCGGG CCC^GGG
Tru9I 290 TTAA T^TAA
Tsp45I 787 GTGAC ^GT[GC]AC
XmaCI 313 CCCGGG C^CCGGG
XmaI 313 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 342 CCGC CCGC(-3/-1)
644 CCGC
AcsI 31 GAATTT [GA]^AATT[CT]
469 GAATTC
ApoI 31 GAATTT [GA]^AATT[CT]
469 GAATTC
BalI 739 TGGCCA TGG^CCA
776 TGGCCA
BamHI 93 GGATCC G^GATCC
124 GGATCC
BcnI 313 CCCGG CC^[GC]GG
314 CCGGG
BsrDI 38 GCAATG GCAATG(2/0)
727 GCAATG
Cfr13I 364 GGCCC G^G[ACGT]CC
858 GGACC
EaeI 739 TGGCCA [CT]^GGCC[GA]
776 TGGCCA
EarI 625 CTCTTC CTCTTC(1/4)
143 GAAGAG
Ksp632I 625 CTCTTC CTCTTC(1/4)
143 GAAGAG
MaeII 385 ACGT A^CGT
451 ACGT
MfeI 782 CAATTG C^AATTG
791 CAATTG
MluNI 739 TGGCCA TGG^CCA
776 TGGCCA
MscI 739 TGGCCA TGG^CCA
776 TGGCCA
MunI 782 CAATTG C^AATTG
791 CAATTG
NciI 313 CCCGG CC^[GC]GG
314 CCGGG
NlaIII 549 CATG CATG^
693 CATG
Sau96I 364 GGCCC G^G[ACGT]CC
858 GGACC
TfiI 414 GAATC G^A[AT]TC
684 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AluI AlwI BbvI BsaJI BsiYI
BslI BssKI BstNI BstYI DdeI
DpnII EcoRII Fnu4HI HaeIII HinfI
ItaI MaeIII MboI MboII MflI
MnlI MvaI NdeII NlaIV Sau3AI
ScrFI SfaNI TseI Tsp509I XhoII