##################################
# NO CUT ENZYMES (  201 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRV       
EcoT14I     EcoT22I     FbaI        FokI        FseI        
FspI        HaeII       HapII       HhaI        HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MamI        MboII       MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        Tru9I       TspRI       Tth111I     Van91I      
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AcsI          316  GAATTC               [GA]^AATT[CT]
AcyI          163  GACGCC               G[GA]^CG[CT]C
ApoI          316  GAATTC               [GA]^AATT[CT]
AvaII         209  GGACC                G^G[AT]CC
BcgI          266  GCATCACCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         310  GGATAC               GTATCC(6/5)
BsaHI         163  GACGCC               G[GA]^CG[CT]C
BsgI          130  CTGCAC               GTGCAG(16/14)
BsmFI         208  GGGAC                GGGAC(10/14)
DdeI           83  CTAAG                C^T[ACGT]AG
DpnII         384  GATC                 ^GATC
DraII         199  GGGGCCT              [GA]G^G[ACGT]CC[CT]
DsaI          156  CCACGG               C^C[GA][CT]GG
EcoO109I      199  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         316  GAATTC               G^AATTC
Hin1I         163  GACGCC               G[GA]^CG[CT]C
HinfI          30  GAATC                G^A[ACGT]TC
MaeIII        374  GTGAC                ^GT[ACGT]AC
MboI          384  GATC                 ^GATC
MslI          171  CACCATCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII         384  GATC                 ^GATC
NlaIII        360  CATG                 CATG^
PvuII         365  CAGCTG               CAG^CTG
Sau3AI        384  GATC                 ^GATC
SfaNI         266  GCATC                GCATC(5/9)
TfiI           30  GAATC                G^A[AT]TC
Tsp45I        374  GTGAC                ^GT[GC]AC
Tsp509I       317  AATT                 ^AATT
XcmI          350  CCATCAACTACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BpmI           41  CTGGAG               CTGGAG(16/14)
              343  CTGGAG              
BsiYI         212  CCGAGCAGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              336  CCTCAACCTGG         
BslI          212  CCGAGCAGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              336  CCTCAACCTGG         
Cac8I          19  GCCAGC               GC[ACGT]^[ACGT]GC
              128  GCCTGC              
Cfr13I        200  GGGCC                G^G[ACGT]CC
              209  GGACC               
FauI          197  GCGGG                CCCGC(4/6)
              219  GCGGG               
HgaI           79  GACGC                GACGC(5/10)
              163  GACGC               
HphI          373  GGTGA                GGTGA(8/7)
              269  TCACC               
MspA1I        217  CAGCGG               C[AC]G^C[GT]G
              365  CAGCTG              
Sau96I        200  GGGCC                G^G[ACGT]CC
              209  GGACC               
TaqI           54  TCGA                 T^CGA
              275  TCGA                
TthHB8I        54  TCGA                 T^CGA
              275  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   16 enzymes) #
#########################################

AciI        AluI        BbvI        BsaJI       BssKI       
BstNI       EcoRII      Fnu4HI      HaeIII      ItaI        
MnlI        MvaI        MwoI        NlaIV       ScrFI       
TseI