Restriction map for
pF1KB8377
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BalI BbeI BbrPI BbsI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BspLU11I BsrBI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CfoI ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoT22I FseI FspI HaeII
HhaI HinP1I HincII HindII HpaI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MseI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspI SwaI TfiI
Tru9I XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcyI 1816 GACGCC G[GA]^CG[CT]C
Asp700I 1828 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 791 GAGCAC G[AT]GC[AT]^C
AspI 367 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1438 CTCGAG C^[CT]CG[GA]G
BamHI 1796 GGATCC G^GATCC
BbvCI 562 GCTGAGG CCTCAGC(-5/-2)
BciVI 1587 GGATAC GTATCC(6/5)
BclI 659 TGATCA T^GATCA
BlpI 1260 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1260 GCTCAGC GC^T[ACGT]AGC
BsaHI 1816 GACGCC G[GA]^CG[CT]C
BsaWI 97 TCCGGT [AT]^CCGG[AT]
BsiHKAI 791 GAGCAC G[AT]GC[AT]^C
BsmBI 1082 GAGACG CGTCTC(1/5)
BsoBI 1438 CTCGAG C^[CT]CG[GA]G
Bsp1407I 743 TGTACA T^GTACA
BspHI 696 TCATGA T^CATGA
BsrFI 62 ACCGGC [GA]^CCGG[CT]
BsrGI 743 TGTACA T^GTACA
CelII 1260 GCTCAGC GC^T[ACGT]AGC
Cfr10I 62 ACCGGC [GA]^CCGG[CT]
DsaI 721 CCACGG C^C[GA][CT]GG
EaeI 1581 CGGCCA [CT]^GGCC[GA]
EcoRV 283 GATATC GAT^ATC
FbaI 659 TGATCA T^GATCA
Hin1I 1816 GACGCC G[GA]^CG[CT]C
HindIII 246 AAGCTT A^AGCTT
PaeR7I 1438 CTCGAG C^TCGAG
PflFI 367 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1404 CCAAAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 696 TCATGA T^CATGA
SapI 1446 GCTCTTC GCTCTTC(1/4)
SmlI 1438 CTCGAG C^T[CT][GA]AG
SspBI 743 TGTACA T^GTACA
Tth111I 367 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1404 CCAAAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1438 CTCGAG C^TCGAG
XmnI 1828 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 1423 GAATTT [GA]^AATT[CT]
1831 GAATTT
ApaI 331 GGGCCC GGGCC^C
1168 GGGCCC
ApoI 1423 GAATTT [GA]^AATT[CT]
1831 GAATTT
BcgI 1430 GCAGGTGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1527 GCAGCAGCTTCG
BcnI 1295 CCCGG CC^[GC]GG
1452 CCGGG
BglI 79 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
422 GCCTGGTTGGC
BstXI 14 CCACCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
29 CCAGCTGCCTGG
BstYI 525 GGATCT [GA]^GATC[CT]
1796 GGATCC
EarI 1447 CTCTTC CTCTTC(1/4)
1674 GAAGAG
EcoNI 816 CCTGAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1368 CCTGCTTCAGG
FauI 5 CCCGC CCCGC(4/6)
954 CCCGC
HgaI 1084 GACGC GACGC(5/10)
1816 GACGC
Ksp632I 1447 CTCTTC CTCTTC(1/4)
1674 GAAGAG
MflI 525 GGATCT [GA]^GATC[CT]
1796 GGATCC
NciI 1295 CCCGG CC^[GC]GG
1452 CCGGG
SbfI 432 CCTGCAGG CCTGCA^GG
588 CCTGCAGG
Sse8387I 432 CCTGCAGG CCTGCA^GG
588 CCTGCAGG
XhoII 525 GGATCT [GA]^GATC[CT]
1796 GGATCC
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AvaII BanI BanII BbvI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Bsu36I Cac8I Cfr13I
DdeI DpnII DraII Eco81I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I