Restriction map for
pF1KB8327
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspHI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BcgI
BciVI BfaI BfrI BlnI BpuAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MroI MseI MslI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AhdI 124 GACAGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 471 GATCC GGATC(4/5)
ApaI 320 GGGCCC GGGCC^C
AspEI 124 GACAGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 154 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 399 TGGCCA TGG^CCA
BclI 191 TGATCA T^GATCA
BglI 41 GCCAGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 542 AGATCT A^GATCT
BlpI 36 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 36 GCTGAGC GC^T[ACGT]AGC
BsaAI 108 CACGTA [CT]AC^GT[GA]
BsaI 382 GAGACC GGTCTC(1/5)
BsmFI 132 GTCCC GGGAC(10/14)
BspMI 113 ACCTGC ACCTGC(4/8)
BstYI 542 AGATCT [GA]^GATC[CT]
CelII 36 GCTGAGC GC^T[ACGT]AGC
DraII 319 GGGGCCC [GA]G^G[ACGT]CC[CT]
EaeI 399 TGGCCA [CT]^GGCC[GA]
Eam1105I 124 GACAGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 319 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 591 CCATGG C^C[AT][AT]GG
FbaI 191 TGATCA T^GATCA
MaeII 109 ACGT A^CGT
MboII 545 TCTTC GAAGA(8/7)
MflI 542 AGATCT [GA]^GATC[CT]
MluNI 399 TGGCCA TGG^CCA
MscI 399 TGGCCA TGG^CCA
NcoI 591 CCATGG C^CATGG
NspI 731 ACATGC [GA]CATG^[CT]
PflFI 154 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 438 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SmaI 134 CCCGGG CCC^GGG
StyI 591 CCATGG C^C[AT][AT]GG
Tth111I 154 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 438 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 542 AGATCT [GA]^GATC[CT]
XmaCI 134 CCCGGG C^CCGGG
XmaI 134 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvaII 246 GGACC G^G[AT]CC
531 GGTCC
BanII 59 GAGCCC G[GA]GC[CT]^C
320 GGGCCC
BbvCI 648 CCTCAGC CCTCAGC(-5/-2)
700 CCTCAGC
BmyI 59 GAGCCC G[AGT]GC[ACT]^C
320 GGGCCC
BpmI 56 CTGGAG CTGGAG(16/14)
455 CTGGAG
BsgI 99 CTGCAC GTGCAG(16/14)
745 GTGCAG
Bsp1286I 59 GAGCCC G[AGT]GC[ACT]^C
320 GGGCCC
Cac8I 41 GCCAGC GC[ACGT]^[ACGT]GC
391 GCCAGC
FauI 308 CCCGC CCCGC(4/6)
50 GCGGG
HaeII 171 AGCGCC [GA]GCGC^[CT]
368 GGCGCT
HapII 27 CCGG C^CGG
135 CCGG
HpaII 27 CCGG C^CGG
135 CCGG
MaeIII 122 GTGAC ^GT[ACGT]AC
418 GTCAC
MspI 27 CCGG C^CGG
135 CCGG
SfaNI 181 GATGC GCATC(5/9)
460 GATGC
TaqI 86 TCGA T^CGA
152 TCGA
Tsp45I 122 GTGAC ^GT[GC]AC
418 GTCAC
TthHB8I 86 TCGA T^CGA
152 TCGA
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
BbvI BcnI BsaJI BseRI BsiYI
BslI BsmAI BsoBI BsrI BssKI
BstF5I BstNI BstUI CfoI Cfr13I
DdeI DpnII DsaI EcoRII Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI MboI MnlI MspA1I
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI Sau96I ScrFI TseI TspRI