##################################
# NO CUT ENZYMES (  169 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbsI        BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        Bpu1102I    BpuAI       
BsaBI       BsaHI       BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsoBI       Bsp1286I    
Bsp1407I    BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      CpoI        DpnII       DraIII      DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HapII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KpnI        KspI        MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflMI       
PinAI       PleI        PmeI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        Sau3AI      SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Van91I      XbaI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AciI          993  GCGG                 CCGC(-3/-1)
Asp700I       183  GAAAATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI           43  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI         785  CACGTG               CAC^GTG
BbvCI          37  GCTGAGG              CCTCAGC(-5/-2)
BfaI          116  CTAG                 C^TAG
BpmI         1072  CTCCAG               CTGGAG(16/14)
BsaAI         785  CACGTG               [CT]AC^GT[GA]
BsaI          746  GAGACC               GGTCTC(1/5)
BseRI        1069  CTCCTC               GAGGAG(10/8)
BsgI          534  CTGCAC               GTGCAG(16/14)
BsmAI         746  GAGAC                GTCTC(1/5)
BsmI         1137  GAATGC               GAATGC(1/-1)
BspDI         583  ATCGAT               AT^CGAT
BstAPI       1132  GCAGAGAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I         536  GCACGC               GC[ACGT]^[ACGT]GC
CfoI          887  GCGC                 GCG^C
ClaI          583  ATCGAT               AT^CGAT
DraI          955  TTTAAA               TTT^AAA
DrdI          325  GACCTTGGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          276  GAAGAG               CTCTTC(1/4)
HhaI          887  GCGC                 GCG^C
HinP1I        887  GCGC                 G^CGC
HinfI         609  GAATC                G^A[ACGT]TC
Ksp632I       276  GAAGAG               CTCTTC(1/4)
MaeI          116  CTAG                 C^TAG
MaeII         786  ACGT                 A^CGT
NspI          576  GCATGT               [GA]CATG^[CT]
PflFI          43  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         785  CACGTG               CAC^GTG
PmlI          785  CACGTG               CAC^GTG
PstI         1130  CTGCAG               CTGCA^G
SapI          276  GAAGAGC              GCTCTTC(1/4)
SexAI          44  ACCTGGT              A^CC[AT]GGT
SfcI         1130  CTGCAG               C^T[GA][CT]AG
SmlI          298  CTCAAG               C^T[CT][GA]AG
StuI         1118  AGGCCT               AGG^CCT
TfiI          609  GAATC                G^A[AT]TC
Tth111I        43  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI           56  CCAAGTATAAGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          183  GAAAATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    2 enzymes) #
###################################

BstXI         161  CCATGATTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              336  CCATGTGGATGG        
PpuMI          41  AGGACCT              [GA]G^G[AT]CC[CT]
              394  GGGACCC             


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        AvaII       
BbvI        BsaJI       BsiYI       BslI        BsmFI       
BsrI        BssKI       BstF5I      BstNI       Cfr13I      
DdeI        DraII       EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HphI        ItaI        
MaeIII      MboII       MnlI        MseI        MslI        
MvaI        MwoI        NlaIII      NlaIV       RsaI        
Sau96I      ScrFI       SfaNI       StyI        TaqI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I