##################################
# NO CUT ENZYMES (  199 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AvaII       
AviII       AvrII       BamHI       BanI        BbeI        
BbrPI       BbvI        BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsiEI       BsiWI       BsmAI       BsmBI       
BsmFI       BsoBI       BspDI       BspEI       BspHI       
BspMI       BsrBI       BsrFI       BssHII      BssKI       
Bst1107I    BstAPI      BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DpnII       DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT14I     EcoT22I     FbaI        Fnu4HI      FokI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        HpaII       ItaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MaeII       MamI        
MboI        MfeI        MflI        MluI        MroI        
MspA1I      MspI        MunI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacII       SalI        SapI        Sau3AI      
Sau96I      SbfI        ScaI        ScrFI       SexAI       
SfaNI       SfcI        SfiI        SfoI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        StyI        SwaI        
TseI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AciI          314  GCGG                 CCGC(-3/-1)
AflIII        603  ACATGT               A^C[GA][CT]GT
AspHI         669  GAGCTC               G[AT]GC[AT]^C
BalI          495  TGGCCA               TGG^CCA
BanII         669  GAGCTC               G[GA]GC[CT]^C
BbsI           70  GTCTTC               GAAGAC(2/6)
BbvCI           9  CCTCAGC              CCTCAGC(-5/-2)
BmyI          669  GAGCTC               G[AGT]GC[ACT]^C
BpuAI          70  GTCTTC               GAAGAC(2/6)
BsgI          807  GTGCAG               GTGCAG(16/14)
BsiHKAI       669  GAGCTC               G[AT]GC[AT]^C
BsiYI         699  CCATACAGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          699  CCATACAGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI          637  GAATGC               GAATGC(1/-1)
Bsp1286I      669  GAGCTC               G[AGT]GC[ACT]^C
Bsp1407I      600  TGTACA               T^GTACA
BspLU11I      603  ACATGT               A^CATGT
BsrDI         641  GCAATG               GCAATG(2/0)
BsrGI         600  TGTACA               T^GTACA
BssSI         114  CACGAG               CACGAG(-5/-1)
BstBI         214  TTCGAA               TT^CGAA
Cac8I          14  GCCAGC               GC[ACGT]^[ACGT]GC
DraI          466  TTTAAA               TTT^AAA
EaeI          495  TGGCCA               [CT]^GGCC[GA]
FauI          314  GCGGG                CCCGC(4/6)
HaeIII        496  GGCC                 GG^CC
HinfI         612  GATTC                G^A[ACGT]TC
HphI          622  GGTGA                GGTGA(8/7)
MluNI         495  TGGCCA               TGG^CCA
MscI          495  TGGCCA               TGG^CCA
MslI          616  CATTCAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspV          214  TTCGAA               TT^CGAA
SacI          669  GAGCTC               GAGCT^C
SfuI          214  TTCGAA               TT^CGAA
SspBI         600  TGTACA               T^GTACA
TaqI          215  TCGA                 T^CGA
TfiI          612  GATTC                G^A[AT]TC
TthHB8I       215  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AfaI          507  GTAC                 GT^AC
              601  GTAC                
BpmI          284  CTGGAG               CTGGAG(16/14)
              536  CTGGAG              
MboII         501  GAAGA                GAAGA(8/7)
               71  TCTTC               
MseI          179  TTAA                 T^TAA
              467  TTAA                
NspI          603  ACATGT               [GA]CATG^[CT]
              687  GCATGT              
RsaI          507  GTAC                 GT^AC
              601  GTAC                
Tru9I         179  TTAA                 T^TAA
              467  TTAA                
Tsp45I          3  GTCAC                ^GT[GC]AC
              840  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   13 enzymes) #
#########################################

AcsI        AluI        ApoI        Asp700I     BsrI        
DdeI        HindIII     MaeIII      MnlI        NlaIII      
Tsp509I     TspRI       XmnI