Restriction map for
pF1KB8281
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AlwNI AocI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII BamHI BanI
BbeI BbrPI BbsI BcgI BciVI
BclI BfrI BglII BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaWI BseAI
BsgI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco81I EcoO65I EcoRI EcoRV FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MroI MspA1I MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccII 169 CGCG CG^CG
Alw44I 487 GTGCAC G^TGCAC
Aor51HI 199 AGCGCT AGC^GCT
ApaLI 487 GTGCAC G^TGCAC
AspHI 487 GTGCAC G[AT]GC[AT]^C
AvrII 727 CCTAGG C^CTAGG
BalI 687 TGGCCA TGG^CCA
BbvCI 299 CCTCAGC CCTCAGC(-5/-2)
BlnI 727 CCTAGG C^CTAGG
BsaAI 619 TACGTA [CT]AC^GT[GA]
BsiEI 171 CGGCCG CG[GA][CT]^CG
BsiHKAI 487 GTGCAC G[AT]GC[AT]^C
BsiWI 617 CGTACG C^GTACG
BstUI 169 CGCG CG^CG
EagI 171 CGGCCG C^GGCCG
EclXI 171 CGGCCG C^GGCCG
Eco47III 199 AGCGCT AGC^GCT
Eco52I 171 CGGCCG C^GGCCG
EcoNI 722 CCTGGCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 589 ATGCAT ATGCA^T
MaeII 620 ACGT A^CGT
MaeIII 514 GTGAC ^GT[ACGT]AC
MluNI 687 TGGCCA TGG^CCA
MscI 687 TGGCCA TGG^CCA
MseI 560 TTAA T^TAA
MvnI 169 CGCG CG^CG
NsiI 589 ATGCAT ATGCA^T
NspI 572 ACATGC [GA]CATG^[CT]
SexAI 223 ACCAGGT A^CC[AT]GGT
SmaI 867 CCCGGG CCC^GGG
SmlI 276 CTCAAG C^T[CT][GA]AG
SnaBI 619 TACGTA TAC^GTA
Tru9I 560 TTAA T^TAA
Tsp45I 514 GTGAC ^GT[GC]AC
XmaCI 867 CCCGGG C^CCGGG
XmaI 867 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 221 GTAC GT^AC
618 GTAC
AvaI 828 CCCGAG C^[CT]CG[GA]G
867 CCCGGG
BanII 71 GGGCTC G[GA]GC[CT]^C
462 GAGCCC
BsaI 227 GGTCTC GGTCTC(1/5)
794 GGTCTC
BseRI 906 CTCCTC GAGGAG(10/8)
810 GAGGAG
BsoBI 828 CCCGAG C^[CT]CG[GA]G
867 CCCGGG
BspMI 791 GCAGGT ACCTGC(4/8)
836 GCAGGT
BsrI 271 ACTGG ACTGG(1/-1)
206 CCAGT
Cac8I 29 GCTTGC GC[ACGT]^[ACGT]GC
714 GCTGGC
DraII 817 AGGTCCC [GA]G^G[ACGT]CC[CT]
838 AGGTCCC
DsaI 578 CCGTGG C^C[GA][CT]GG
802 CCACGG
EaeI 171 CGGCCG [CT]^GGCC[GA]
687 TGGCCA
EcoO109I 817 AGGTCCC [GA]G^G[ACGT]CC[CT]
838 AGGTCCC
HphI 79 TCACC GGTGA(8/7)
503 TCACC
PpuMI 817 AGGTCCC [GA]G^G[AT]CC[CT]
838 AGGTCCC
RsaI 221 GTAC GT^AC
618 GTAC
TaqI 154 TCGA T^CGA
241 TCGA
TfiI 633 GAATC G^A[AT]TC
746 GATTC
Tsp509I 558 AATT ^AATT
681 AATT
TthHB8I 154 TCGA T^CGA
241 TCGA
XcmI 704 CCACCGCCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
775 CCACCGCCTATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BcnI BfaI BglI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrBI BssKI BstF5I BstNI
CfoI Cfr13I DdeI DpnII EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI Ksp632I MaeI
MboI MboII MnlI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI SapI Sau3AI Sau96I
ScrFI SfaNI StyI TseI TspRI