Restriction map for
pF1KB8279
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AflIII AgeI Alw44I AlwI AlwNI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspI AviII AvrII
BamHI BanI BbeI BbrPI BcgI
BclI BcnI BfaI BglI BlnI
BlpI Bpu1102I BsaAI BsiEI BsiWI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI BstBI
BstEII BstPI BstUI CelII ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI EarI EclXI Eco52I
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HincII
HindII HpaI KasI KpnI Ksp632I
KspI MaeI MaeII MfeI MluI
MspA1I MunI MvnI NarI NciI
NcoI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 542 GTATAC GT^[AC][GT]AC
AccIII 309 TCCGGA T^CCGGA
AcyI 710 GACGCC G[GA]^CG[CT]C
AhdI 147 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 274 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1046 AGCGCT AGC^GCT
AspEI 147 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 158 CCCGAG C^[CT]CG[GA]G
BalI 99 TGGCCA TGG^CCA
BanII 280 GAGCTC G[GA]GC[CT]^C
BbsI 49 GAAGAC GAAGAC(2/6)
BbvCI 999 CCTCAGC CCTCAGC(-5/-2)
BciVI 1028 GGATAC GTATCC(6/5)
BglII 345 AGATCT A^GATCT
BpuAI 49 GAAGAC GAAGAC(2/6)
BsaBI 494 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 710 GACGCC G[GA]^CG[CT]C
BsaI 1212 GAGACC GGTCTC(1/5)
BsaWI 309 TCCGGA [AT]^CCGG[AT]
BseAI 309 TCCGGA T^CCGGA
BsmBI 708 GAGACG CGTCTC(1/5)
BsoBI 158 CCCGAG C^[CT]CG[GA]G
BspEI 309 TCCGGA T^CCGGA
BsrFI 173 GCCGGC [GA]^CCGG[CT]
Bst1107I 542 GTATAC GTA^TAC
BstAPI 1142 GCAGTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 390 CCATGAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 345 AGATCT [GA]^GATC[CT]
BstZ17I 542 GTATAC GTA^TAC
Bsu36I 274 CCTGAGG CC^T[ACGT]AGG
CfoI 1047 GCGC GCG^C
Cfr10I 173 GCCGGC [GA]^CCGG[CT]
EaeI 99 TGGCCA [CT]^GGCC[GA]
Eam1105I 147 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1046 AGCGCT AGC^GCT
Eco81I 274 CCTGAGG CC^T[ACGT]AGG
EcoNI 270 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 297 GCGGG CCCGC(4/6)
HaeII 1046 AGCGCT [GA]GCGC^[CT]
HgaI 710 GACGC GACGC(5/10)
HhaI 1047 GCGC GCG^C
Hin1I 710 GACGCC G[GA]^CG[CT]C
HinP1I 1047 GCGC G^CGC
HindIII 738 AAGCTT A^AGCTT
MamI 494 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 345 AGATCT [GA]^GATC[CT]
MluNI 99 TGGCCA TGG^CCA
MroI 309 TCCGGA T^CCGGA
MscI 99 TGGCCA TGG^CCA
NaeI 173 GCCGGC GCC^GGC
NgoMIV 173 GCCGGC G^CCGGC
PflMI 555 CCAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 280 GAGCTC GAGCT^C
ScaI 756 AGTACT AGT^ACT
SfcI 335 CTGTAG C^T[GA][CT]AG
Tsp45I 481 GTGAC ^GT[GC]AC
Van91I 555 CCAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 345 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflII 28 CTTAAG C^TTAAG
1180 CTTAAG
AvaII 63 GGACC G^G[AT]CC
154 GGTCC
BbvI 684 GCAGC GCAGC(8/12)
240 GCTGC
BfrI 28 CTTAAG C^TTAAG
1180 CTTAAG
BsaJI 290 CCTGGG C^C[ACGT][ACGT]GG
370 CCAGGG
BsgI 1140 GTGCAG GTGCAG(16/14)
1149 GTGCAG
BsmFI 62 GGGAC GGGAC(10/14)
155 GTCCC
BsmI 970 GAATGC GAATGC(1/-1)
1086 GAATGC
BsrDI 722 GCAATG GCAATG(2/0)
920 GCAATG
BstF5I 307 CATCC GGATG(2/0)
423 GGATG
Cfr13I 63 GGACC G^G[ACGT]CC
154 GGTCC
DpnII 25 GATC ^GATC
346 GATC
Fnu4HI 240 GCTGC GC^[ACGT]GC
684 GCAGC
FokI 307 CATCC GGATG(9/13)
423 GGATG
ItaI 240 GCTGC GC^[ACGT]GC
684 GCAGC
MaeIII 137 GTTAC ^GT[ACGT]AC
481 GTGAC
MboI 25 GATC ^GATC
346 GATC
NdeI 797 CATATG CA^TATG
965 CATATG
NdeII 25 GATC ^GATC
346 GATC
Sau3AI 25 GATC ^GATC
346 GATC
Sau96I 63 GGACC G^G[ACGT]CC
154 GGTCC
SmlI 28 CTTAAG C^T[CT][GA]AG
1180 CTTAAG
StuI 287 AGGCCT AGG^CCT
821 AGGCCT
TaqI 81 TCGA T^CGA
933 TCGA
TseI 240 GCTGC G^C[AT]GC
684 GCAGC
Tsp509I 257 AATT ^AATT
518 AATT
TthHB8I 81 TCGA T^CGA
933 TCGA
XcmI 141 CCAAGAGACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
306 CCATCCGGAGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AfaI AluI Asp700I AspHI
BmyI BpmI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstNI Cac8I DdeI EcoRII HaeIII
HapII HinfI HpaII HphI MboII
MnlI MseI MslI MspI MvaI
MwoI NlaIII NlaIV PleI RsaI
ScrFI SfaNI TfiI Tru9I TspRI
XmnI