Restriction map for
pF1KB8269
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# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbvCI
BcgI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiWI BsmBI BsmFI BsmI BsoBI
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI HphI
KasI KpnI Ksp632I KspI MaeI
MaeIII MamI MfeI MflI MluI
MroI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tsp45I Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AciI 62 CCGC CCGC(-3/-1)
AocI 477 CCTTAGG CC^T[ACGT]AGG
Aor51HI 339 AGCGCT AGC^GCT
AspHI 496 GAGCTC G[AT]GC[AT]^C
AvaII 284 GGACC G^G[AT]CC
BalI 371 TGGCCA TGG^CCA
BanI 403 GGTGCC G^G[CT][GA]CC
BbrPI 455 CACGTG CAC^GTG
BbsI 272 GTCTTC GAAGAC(2/6)
BcnI 54 CCGGG CC^[GC]GG
BpuAI 272 GTCTTC GAAGAC(2/6)
BsaAI 455 CACGTG [CT]AC^GT[GA]
BsiHKAI 496 GAGCTC G[AT]GC[AT]^C
Bsp1407I 159 TGTACA T^GTACA
BspHI 93 TCATGA T^CATGA
BsrGI 159 TGTACA T^GTACA
BstBI 539 TTCGAA TT^CGAA
BstXI 393 CCACGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 477 CCTTAGG CC^T[ACGT]AGG
CfoI 340 GCGC GCG^C
DpnII 91 GATC ^GATC
Eco47III 339 AGCGCT AGC^GCT
Eco81I 477 CCTTAGG CC^T[ACGT]AGG
HaeII 339 AGCGCT [GA]GCGC^[CT]
HapII 54 CCGG C^CGG
HhaI 340 GCGC GCG^C
HinP1I 340 GCGC G^CGC
HpaII 54 CCGG C^CGG
MboI 91 GATC ^GATC
MboII 273 TCTTC GAAGA(8/7)
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
MseI 107 TTAA T^TAA
MslI 394 CACGGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 54 CCGG C^CGG
NciI 54 CCGGG CC^[GC]GG
NdeII 91 GATC ^GATC
NspV 539 TTCGAA TT^CGAA
PflMI 564 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 455 CACGTG CAC^GTG
PmlI 455 CACGTG CAC^GTG
RcaI 93 TCATGA T^CATGA
SacI 496 GAGCTC GAGCT^C
Sau3AI 91 GATC ^GATC
SfuI 539 TTCGAA TT^CGAA
SspBI 159 TGTACA T^GTACA
SspI 70 AATATT AAT^ATT
Tru9I 107 TTAA T^TAA
Tsp509I 221 AATT ^AATT
Van91I 564 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 6 GTAC GT^AC
160 GTAC
AluI 240 AGCT AG^CT
497 AGCT
Asp700I 172 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
504 GAAACGCTTC
BanII 496 GAGCTC G[GA]GC[CT]^C
597 GGGCTC
BciVI 17 GTATCC GTATCC(6/5)
422 GTATCC
BmyI 496 GAGCTC G[AGT]GC[ACT]^C
597 GGGCTC
BseRI 304 CTCCTC GAGGAG(10/8)
493 GAGGAG
BsiEI 51 CGACCG CG[GA][CT]^CG
429 CGACCG
BsmAI 152 GAGAC GTCTC(1/5)
225 GTCTC
Bsp1286I 496 GAGCTC G[AGT]GC[ACT]^C
597 GGGCTC
BstF5I 513 CATCC GGATG(2/0)
561 CATCC
DdeI 418 CTCAG C^T[ACGT]AG
478 CTTAG
DraII 24 AGGCCCC [GA]G^G[ACGT]CC[CT]
377 AGGCCCC
DsaI 230 CCACGG C^C[GA][CT]GG
393 CCACGG
EaeI 59 TGGCCG [CT]^GGCC[GA]
371 TGGCCA
EcoO109I 24 AGGCCCC [GA]G^G[ACGT]CC[CT]
377 AGGCCCC
FokI 513 CATCC GGATG(9/13)
561 CATCC
MaeII 188 ACGT A^CGT
456 ACGT
PleI 414 GACTC GAGTC(4/5)
279 GAGTC
RsaI 6 GTAC GT^AC
160 GTAC
SfaNI 204 GATGC GCATC(5/9)
560 GCATC
TaqI 428 TCGA T^CGA
540 TCGA
TfiI 519 GAATC G^A[AT]TC
542 GAATC
TthHB8I 428 TCGA T^CGA
540 TCGA
XmnI 172 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
504 GAAACGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
BbvI BpmI BsaJI BsiYI BslI
BssKI BstNI Cac8I Cfr13I EcoRII
EcoT14I Fnu4HI HaeIII HinfI ItaI
MnlI MvaI MwoI NlaIII NlaIV
Sau96I ScrFI StyI TseI TspRI