Restriction map for
pF1KB8268
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# NO CUT ENZYMES ( 184 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AvaII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BlnI BpmI BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsgI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstZ17I Cac8I Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DsaI
Eam1105I Eco47III EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FokI FseI HaeII HapII HgaI
Hin1I HincII HindII HindIII HpaI
HpaII HphI KasI KpnI KspI
MaeII MaeIII MamI MluI MluNI
MroI MscI MslI MspA1I MspI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
Sau96I SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tsp45I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AciI 4 GCGG CCGC(-3/-1)
AfaI 132 GTAC GT^AC
AocI 244 CCTGAGG CC^T[ACGT]AGG
AspHI 124 GAGCAC G[AT]GC[AT]^C
AviII 41 TGCGCA TGC^GCA
BbvI 39 GCTGC GCAGC(8/12)
BglII 204 AGATCT A^GATCT
BlpI 277 GCTAAGC GC^T[ACGT]AGC
BmyI 124 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 277 GCTAAGC GC^T[ACGT]AGC
BsiEI 5 CGGCCG CG[GA][CT]^CG
BsiHKAI 124 GAGCAC G[AT]GC[AT]^C
BsiYI 359 CCTTGGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 359 CCTTGGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 273 GAATGC GAATGC(1/-1)
Bsp1286I 124 GAGCAC G[AGT]GC[ACT]^C
BssKI 474 CCAGG ^CC[ACGT]GG
BstNI 474 CCAGG CC^[AT]GG
BstYI 204 AGATCT [GA]^GATC[CT]
Bsu36I 244 CCTGAGG CC^T[ACGT]AGG
CelII 277 GCTAAGC GC^T[ACGT]AGC
CfoI 42 GCGC GCG^C
DpnII 205 GATC ^GATC
DrdI 18 GACAAAGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 5 CGGCCG [CT]^GGCC[GA]
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
Eco81I 244 CCTGAGG CC^T[ACGT]AGG
EcoRII 474 CCAGG ^CC[AT]GG
EcoT14I 359 CCTTGG C^C[AT][AT]GG
FspI 41 TGCGCA TGC^GCA
HaeIII 6 GGCC GG^CC
HhaI 42 GCGC GCG^C
HinP1I 42 GCGC G^CGC
MboI 205 GATC ^GATC
MfeI 371 CAATTG C^AATTG
MflI 204 AGATCT [GA]^GATC[CT]
MunI 371 CAATTG C^AATTG
MvaI 474 CCAGG CC^[AT]GG
NdeII 205 GATC ^GATC
PleI 70 GACTC GAGTC(4/5)
RsaI 132 GTAC GT^AC
Sau3AI 205 GATC ^GATC
ScrFI 474 CCAGG CC^[ACGT]GG
SfaNI 261 GATGC GCATC(5/9)
StyI 359 CCTTGG C^C[AT][AT]GG
TfiI 33 GAATC G^A[AT]TC
TseI 39 GCTGC G^C[AT]GC
TspRI 127 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 204 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AluI 149 AGCT AG^CT
317 AGCT
Asp700I 202 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
456 GAAGATGTTC
BfaI 91 CTAG C^TAG
344 CTAG
BsaI 355 GAGACC GGTCTC(1/5)
398 GAGACC
BsaJI 359 CCTTGG C^C[ACGT][ACGT]GG
473 CCCAGG
BsmFI 63 GGGAC GGGAC(10/14)
367 GGGAC
BsrI 66 ACTGG ACTGG(1/-1)
128 ACTGG
Fnu4HI 4 GCGGC GC^[ACGT]GC
39 GCTGC
HinfI 33 GAATC G^A[ACGT]TC
70 GACTC
ItaI 4 GCGGC GC^[ACGT]GC
39 GCTGC
MaeI 91 CTAG C^TAG
344 CTAG
MnlI 233 GAGG CCTC(7/6)
247 GAGG
NlaIII 45 CATG CATG^
403 CATG
SapI 121 GAAGAGC GCTCTTC(1/4)
178 GAAGAGC
XmnI 202 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
456 GAAGATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
#########################################
BsmAI DdeI EarI Ksp632I MboII
MseI Tru9I Tsp509I