Restriction map for
pF1KB8266
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BclI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DpnII DraI DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I MaeI MaeII MaeIII
MamI MboI MfeI MflI MluI
MroI MslI MunI NaeI NarI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SalI SapI Sau3AI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspI
StuI StyI SwaI TfiI Tsp45I
Tth111I Van91I XbaI XcmI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 484 AAATTC [GA]^AATT[CT]
AocI 249 CCTCAGG CC^T[ACGT]AGG
ApaI 411 GGGCCC GGGCC^C
ApoI 484 AAATTC [GA]^AATT[CT]
AvaI 153 CTCGAG C^[CT]CG[GA]G
AvaII 299 GGTCC G^G[AT]CC
BalI 434 TGGCCA TGG^CCA
BanII 411 GGGCCC G[GA]GC[CT]^C
BbsI 532 GTCTTC GAAGAC(2/6)
BciVI 522 GGATAC GTATCC(6/5)
BcnI 165 CCGGG CC^[GC]GG
BmyI 411 GGGCCC G[AGT]GC[ACT]^C
BpuAI 532 GTCTTC GAAGAC(2/6)
BsgI 88 GTGCAG GTGCAG(16/14)
BsoBI 153 CTCGAG C^[CT]CG[GA]G
Bsp1286I 411 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 392 TGTACA T^GTACA
BspMI 56 ACCTGC ACCTGC(4/8)
BsrGI 392 TGTACA T^GTACA
Bsu36I 249 CCTCAGG CC^T[ACGT]AGG
DraIII 15 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 434 TGGCCA [CT]^GGCC[GA]
Eco81I 249 CCTCAGG CC^T[ACGT]AGG
EcoNI 245 CCTTCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 122 GGCGCT [GA]GCGC^[CT]
HinfI 324 GAGTC G^A[ACGT]TC
HphI 488 TCACC GGTGA(8/7)
KspI 293 CCGCGG CCGC^GG
MluNI 434 TGGCCA TGG^CCA
MscI 434 TGGCCA TGG^CCA
MseI 475 TTAA T^TAA
MspA1I 293 CCGCGG C[AC]G^C[GT]G
NciI 165 CCGGG CC^[GC]GG
PaeR7I 153 CTCGAG C^TCGAG
PleI 324 GAGTC GAGTC(4/5)
PpuMI 298 GGGTCCC [GA]G^G[AT]CC[CT]
SacII 293 CCGCGG CCGC^GG
SbfI 57 CCTGCAGG CCTGCA^GG
SmlI 153 CTCGAG C^T[CT][GA]AG
Sse8387I 57 CCTGCAGG CCTGCA^GG
SspBI 392 TGTACA T^GTACA
TaqI 154 TCGA T^CGA
Tru9I 475 TTAA T^TAA
Tsp509I 485 AATT ^AATT
TthHB8I 154 TCGA T^CGA
XhoI 153 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AfaI 381 GTAC GT^AC
393 GTAC
AluI 157 AGCT AG^CT
316 AGCT
BsaWI 69 ACCGGA [AT]^CCGG[AT]
320 ACCGGA
BstF5I 23 GGATG GGATG(2/0)
219 GGATG
DdeI 250 CTCAG C^T[ACGT]AG
470 CTGAG
DsaI 215 CCACGG C^C[GA][CT]GG
293 CCGCGG
FokI 23 GGATG GGATG(9/13)
219 GGATG
MboII 171 GAAGA GAAGA(8/7)
533 TCTTC
PstI 58 CTGCAG CTGCA^G
286 CTGCAG
RsaI 381 GTAC GT^AC
393 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AccII AciI BbvI BsaI BsaJI
BsiYI BslI BsmAI BsmFI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DraII EcoO109I EcoRII FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI MnlI MspI MvaI
MvnI MwoI NlaIII NlaIV Sau96I
ScrFI SfcI TseI TspRI