Restriction map for
pF1KB8263
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BamHI BanII BbeI
BbrPI BciVI BclI BfrI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaHI BsgI BsiHKAI BsiWI BsmBI
BspDI BspHI BspLU11I BspMI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI KspI MaeII MfeI MluI
MspA1I MunI MvnI NaeI NarI
NcoI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 546 TCCGGA T^CCGGA
AluI 428 AGCT AG^CT
AlwI 462 GATCC GGATC(4/5)
Asp700I 411 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 608 TGGCCA TGG^CCA
BanI 511 GGCACC G^G[CT][GA]CC
BbsI 286 GTCTTC GAAGAC(2/6)
BbvCI 818 CCTCAGC CCTCAGC(-5/-2)
BcgI 671 GCATGCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 510 GGGCAC G[AGT]GC[ACT]^C
BpuAI 286 GTCTTC GAAGAC(2/6)
BsaBI 462 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 546 TCCGGA [AT]^CCGG[AT]
BseAI 546 TCCGGA T^CCGGA
BsiEI 39 CGGCCG CG[GA][CT]^CG
BsmI 744 GAATGC GAATGC(1/-1)
Bsp1286I 510 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 935 TGTACA T^GTACA
BspEI 546 TCCGGA T^CCGGA
BsrDI 682 GCAATG GCAATG(2/0)
BsrGI 935 TGTACA T^GTACA
BstYI 461 AGATCC [GA]^GATC[CT]
DraIII 775 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 39 CGGCCG C^GGCCG
EclXI 39 CGGCCG C^GGCCG
Eco52I 39 CGGCCG C^GGCCG
EcoT14I 950 CCAAGG C^C[AT][AT]GG
FauI 92 GCGGG CCCGC(4/6)
HincII 888 GTTGAC GT[CT]^[GA]AC
HindII 888 GTTGAC GT[CT]^[GA]AC
MamI 462 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 461 AGATCC [GA]^GATC[CT]
MluNI 608 TGGCCA TGG^CCA
MroI 546 TCCGGA T^CCGGA
MscI 608 TGGCCA TGG^CCA
NdeI 902 CATATG CA^TATG
NheI 429 GCTAGC G^CTAGC
NspI 671 GCATGC [GA]CATG^[CT]
PflMI 915 CCATGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SexAI 329 ACCTGGT A^CC[AT]GGT
SmlI 895 CTTGAG C^T[CT][GA]AG
SphI 671 GCATGC GCATG^C
SspBI 935 TGTACA T^GTACA
StyI 950 CCAAGG C^C[AT][AT]GG
Tsp509I 393 AATT ^AATT
TspRI 314 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 915 CCATGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 461 AGATCC [GA]^GATC[CT]
XmnI 411 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 399 GTAC GT^AC
936 GTAC
AvaI 496 CTCGGG C^[CT]CG[GA]G
507 CTCGGG
BfaI 430 CTAG C^TAG
556 CTAG
BsaI 911 GAGACC GGTCTC(1/5)
519 GGTCTC
BsmFI 17 GTCCC GGGAC(10/14)
334 GTCCC
BsoBI 496 CTCGGG C^[CT]CG[GA]G
507 CTCGGG
BsrBI 420 CCGCTC CCGCTC(-3/-3)
582 CCGCTC
BsrI 315 ACTGG ACTGG(1/-1)
240 CCAGT
BstEII 55 GGTGACC G^GT[ACGT]ACC
318 GGTCACC
BstPI 55 GGTGACC G^GT[ACGT]ACC
318 GGTCACC
Cac8I 429 GCTAGC GC[ACGT]^[ACGT]GC
671 GCATGC
DdeI 482 CTAAG C^T[ACGT]AG
819 CTCAG
EcoO65I 55 GGTGACC G^GT[ACGT]ACC
318 GGTCACC
MaeI 430 CTAG C^TAG
556 CTAG
MseI 602 TTAA T^TAA
791 TTAA
PleI 808 GACTC GAGTC(4/5)
365 GAGTC
RsaI 399 GTAC GT^AC
936 GTAC
TfiI 11 GATTC G^A[AT]TC
343 GAATC
Tru9I 602 TTAA T^TAA
791 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AvaII BbvI BcnI BglI
BsaJI BseRI BsiYI BslI BsmAI
BssKI BstF5I BstNI BstXI Cfr13I
DpnII DsaI EaeI EarI EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TaqI TseI Tsp45I TthHB8I