Restriction map for
pF1KB8261
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AvaII AviII
AvrII BalI BamHI BanII BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaWI BseAI BseRI
BsgI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DraIII DrdI Eam1105I
EarI Eco47III Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI HphI Ksp632I KspI MaeI
MaeII MaeIII MfeI MflI MluI
MluNI MroI MscI MseI MunI
MvnI NciI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tsp45I Tsp509I Tth111I TthHB8I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 112 GGTACC G^GTACC
AfaI 113 GTAC GT^AC
AlwI 393 GATCC GGATC(4/5)
Asp718I 112 GGTACC G^GTACC
AspHI 418 GTGCTC G[AT]GC[AT]^C
AvaI 22 CCCGAG C^[CT]CG[GA]G
BbsI 70 GTCTTC GAAGAC(2/6)
BglI 299 GCCTGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 271 CTCCAG CTGGAG(16/14)
BpuAI 70 GTCTTC GAAGAC(2/6)
BsaBI 399 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 433 GGTCTC GGTCTC(1/5)
BsiHKAI 418 GTGCTC G[AT]GC[AT]^C
BsmAI 434 GTCTC GTCTC(1/5)
BsmFI 341 GGGAC GGGAC(10/14)
BsoBI 22 CCCGAG C^[CT]CG[GA]G
BspHI 155 TCATGA T^CATGA
BsrDI 120 CATTGC GCAATG(2/0)
BsrI 172 CCAGT ACTGG(1/-1)
DsaI 65 CCATGG C^C[GA][CT]GG
EcoT14I 65 CCATGG C^C[AT][AT]GG
FauI 354 GCGGG CCCGC(4/6)
HgaI 343 GACGC GACGC(5/10)
KpnI 112 GGTACC GGTAC^C
MamI 399 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 168 CATCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 274 CAGCTG C[AC]G^C[GT]G
NcoI 65 CCATGG C^CATGG
PleI 7 GAGTC GAGTC(4/5)
PvuII 274 CAGCTG CAG^CTG
RcaI 155 TCATGA T^CATGA
RsaI 113 GTAC GT^AC
SfiI 298 GGCCTGAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI 65 CCATGG C^C[AT][AT]GG
TfiI 399 GATTC G^A[AT]TC
TspRI 173 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 52 GGCGCC G[GA]^CG[CT]C
280 GGCGCC
AluI 265 AGCT AG^CT
275 AGCT
BbeI 52 GGCGCC GGCGC^C
280 GGCGCC
BmyI 357 GGGCAC G[AGT]GC[ACT]^C
418 GTGCTC
BsaHI 52 GGCGCC G[GA]^CG[CT]C
280 GGCGCC
BsiEI 59 CGGCCG CG[GA][CT]^CG
142 CGGCCG
Bsp1286I 357 GGGCAC G[AGT]GC[ACT]^C
418 GTGCTC
BstF5I 168 CATCC GGATG(2/0)
450 CATCC
Cfr13I 19 GGCCC G^G[ACGT]CC
297 GGGCC
DdeI 289 CTGAG C^T[ACGT]AG
301 CTGAG
DpnII 153 GATC ^GATC
393 GATC
EagI 59 CGGCCG C^GGCCG
142 CGGCCG
EclXI 59 CGGCCG C^GGCCG
142 CGGCCG
Eco52I 59 CGGCCG C^GGCCG
142 CGGCCG
FokI 168 CATCC GGATG(9/13)
450 CATCC
Hin1I 52 GGCGCC G[GA]^CG[CT]C
280 GGCGCC
HinfI 7 GAGTC G^A[ACGT]TC
399 GATTC
KasI 52 GGCGCC G^GCGCC
280 GGCGCC
MboI 153 GATC ^GATC
393 GATC
MboII 71 TCTTC GAAGA(8/7)
407 TCTTC
NaeI 283 GCCGGC GCC^GGC
316 GCCGGC
NarI 52 GGCGCC GG^CGCC
280 GGCGCC
NdeII 153 GATC ^GATC
393 GATC
NgoMIV 283 GCCGGC G^CCGGC
316 GCCGGC
Sau3AI 153 GATC ^GATC
393 GATC
Sau96I 19 GGCCC G^G[ACGT]CC
297 GGGCC
SfaNI 188 GCATC GCATC(5/9)
332 GCATC
SfoI 52 GGCGCC GGC^GCC
280 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI BanI BbvI BsaJI BsiYI
BslI BsrBI BsrFI BssKI BstNI
Cac8I CfoI Cfr10I EaeI EcoRII
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HpaII ItaI MnlI MspI
MvaI MwoI NlaIII NlaIV ScrFI
TseI XcmI