Restriction map for
pF1KB8258
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BsaAI BsaBI BsaHI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CfoI ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MflI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcsI 688 AAATTC [GA]^AATT[CT]
AlwI 621 GATCC GGATC(4/5)
ApoI 688 AAATTC [GA]^AATT[CT]
AspHI 495 GAGCTC G[AT]GC[AT]^C
AvaI 23 CTCGGG C^[CT]CG[GA]G
BalI 587 TGGCCA TGG^CCA
BanII 495 GAGCTC G[GA]GC[CT]^C
BbsI 376 GAAGAC GAAGAC(2/6)
BfaI 16 CTAG C^TAG
BlpI 242 GCTCAGC GC^T[ACGT]AGC
BmyI 495 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 242 GCTCAGC GC^T[ACGT]AGC
BpuAI 376 GAAGAC GAAGAC(2/6)
BsaI 674 GAGACC GGTCTC(1/5)
BseRI 614 GAGGAG GAGGAG(10/8)
BsgI 118 GTGCAG GTGCAG(16/14)
BsiEI 216 CGACCG CG[GA][CT]^CG
BsiHKAI 495 GAGCTC G[AT]GC[AT]^C
BsmAI 674 GAGAC GTCTC(1/5)
BsoBI 23 CTCGGG C^[CT]CG[GA]G
Bsp1286I 495 GAGCTC G[AGT]GC[ACT]^C
BspMI 429 GCAGGT ACCTGC(4/8)
BsrDI 636 CATTGC GCAATG(2/0)
BsrFI 42 ACCGGC [GA]^CCGG[CT]
BstF5I 600 GGATG GGATG(2/0)
BstXI 104 CCACTATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 242 GCTCAGC GC^T[ACGT]AGC
Cfr10I 42 ACCGGC [GA]^CCGG[CT]
DpnII 621 GATC ^GATC
DsaI 590 CCATGG C^C[GA][CT]GG
EarI 550 GAAGAG CTCTTC(1/4)
FokI 600 GGATG GGATG(9/13)
HphI 278 TCACC GGTGA(8/7)
Ksp632I 550 GAAGAG CTCTTC(1/4)
MaeI 16 CTAG C^TAG
MboI 621 GATC ^GATC
MluNI 587 TGGCCA TGG^CCA
MscI 587 TGGCCA TGG^CCA
MseI 350 TTAA T^TAA
MspA1I 245 CAGCTG C[AC]G^C[GT]G
NcoI 590 CCATGG C^CATGG
NdeII 621 GATC ^GATC
PvuII 245 CAGCTG CAG^CTG
SacI 495 GAGCTC GAGCT^C
Sau3AI 621 GATC ^GATC
ScaI 663 AGTACT AGT^ACT
SmlI 416 CTCAAG C^T[CT][GA]AG
TaqI 186 TCGA T^CGA
TfiI 392 GAATC G^A[AT]TC
Tru9I 350 TTAA T^TAA
TthHB8I 186 TCGA T^CGA
XcmI 37 CCAGCACCGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AfaI 435 GTAC GT^AC
664 GTAC
AlwNI 245 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
263 CAGTAGCTG
BsmFI 100 GGGAC GGGAC(10/14)
410 GGGAC
BsrBI 595 GAGCGG CCGCTC(-3/-3)
655 GAGCGG
BstAPI 221 GCATTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
631 GCAGCCATTGC
BstNI 258 CCAGG CC^[AT]GG
627 CCTGG
DraII 169 GGGTCCT [GA]G^G[ACGT]CC[CT]
410 GGGACCC
EaeI 113 TGGCCG [CT]^GGCC[GA]
587 TGGCCA
EcoNI 345 CCTCTTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
415 CCTCAAGAAGG
EcoO109I 169 GGGTCCT [GA]G^G[ACGT]CC[CT]
410 GGGACCC
EcoRII 258 CCAGG ^CC[AT]GG
627 CCTGG
EcoT14I 545 CCAAGG C^C[AT][AT]GG
590 CCATGG
MboII 376 GAAGA GAAGA(8/7)
550 GAAGA
MslI 105 CACTATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
111 CATGGCCGTG
MvaI 258 CCAGG CC^[AT]GG
627 CCTGG
PleI 151 GACTC GAGTC(4/5)
643 GAGTC
PpuMI 169 GGGTCCT [GA]G^G[AT]CC[CT]
410 GGGACCC
RsaI 435 GTAC GT^AC
664 GTAC
SfaNI 271 GATGC GCATC(5/9)
520 GATGC
StyI 545 CCAAGG C^C[AT][AT]GG
590 CCATGG
Tsp45I 190 GTGAC ^GT[GC]AC
277 GTCAC
Tsp509I 563 AATT ^AATT
689 AATT
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BpmI BsaJI BsiYI BslI BsrI
BssKI Cac8I Cfr13I DdeI FauI
Fnu4HI HaeIII HapII HinfI HpaII
ItaI MaeIII MnlI MspI MwoI
NciI NlaIII NlaIV Sau96I ScrFI
TseI TspRI