Restriction map for
pF1KB8256
##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspHI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BciVI
BclI BfrI BglII BlnI BsaAI
BsaBI BsaHI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HindIII HpaI KasI
KpnI MamI MluI MroI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SbfI SexAI SfaNI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccII 8 CGCG CG^CG
AcsI 277 GAATTC [GA]^AATT[CT]
AfaI 972 GTAC GT^AC
AgeI 995 ACCGGT A^CCGGT
AhdI 157 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 277 GAATTC [GA]^AATT[CT]
Asp700I 895 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 157 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 635 CCCGGG C^[CT]CG[GA]G
BalI 290 TGGCCA TGG^CCA
BcgI 104 GCAGCGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 925 CTAG C^TAG
BglI 485 GCCAGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 451 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 451 GCTCAGC GC^T[ACGT]AGC
BsaWI 995 ACCGGT [AT]^CCGG[AT]
BseRI 612 GAGGAG GAGGAG(10/8)
BsoBI 635 CCCGGG C^[CT]CG[GA]G
BspMI 500 ACCTGC ACCTGC(4/8)
BstUI 8 CGCG CG^CG
BstYI 962 AGATCC [GA]^GATC[CT]
CelII 451 GCTCAGC GC^T[ACGT]AGC
DraII 71 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 7 CCGCGG C^C[GA][CT]GG
Eam1105I 157 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 397 GAAGAG CTCTTC(1/4)
EcoO109I 71 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 277 GAATTC G^AATTC
FauI 6 CCCGC CCCGC(4/6)
HincII 831 GTCGAC GT[CT]^[GA]AC
HindII 831 GTCGAC GT[CT]^[GA]AC
Ksp632I 397 GAAGAG CTCTTC(1/4)
KspI 7 CCGCGG CCGC^GG
MaeI 925 CTAG C^TAG
MfeI 836 CAATTG C^AATTG
MflI 962 AGATCC [GA]^GATC[CT]
MluNI 290 TGGCCA TGG^CCA
MscI 290 TGGCCA TGG^CCA
MseI 881 TTAA T^TAA
MunI 836 CAATTG C^AATTG
MvnI 8 CGCG CG^CG
NaeI 494 GCCGGC GCC^GGC
NgoMIV 494 GCCGGC G^CCGGC
PflMI 992 CCAACCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 995 ACCGGT A^CCGGT
PpuMI 71 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 972 GTAC GT^AC
SacII 7 CCGCGG CCGC^GG
SalI 831 GTCGAC G^TCGAC
SapI 397 GAAGAGC GCTCTTC(1/4)
ScaI 971 AGTACT AGT^ACT
SmaI 635 CCCGGG CCC^GGG
SmlI 967 CTCAAG C^T[CT][GA]AG
Tru9I 881 TTAA T^TAA
Van91I 992 CCAACCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 962 AGATCC [GA]^GATC[CT]
XmaCI 635 CCCGGG C^CCGGG
XmaI 635 CCCGGG C^CCGGG
XmnI 895 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 46 GTCTAC GT^[AC][GT]AC
831 GTCGAC
AlwNI 70 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
760 CAGCAGCTG
BanI 497 GGCACC G^G[CT][GA]CC
639 GGCACC
BanII 849 GGGCTC G[GA]GC[CT]^C
929 GAGCCC
BbsI 1033 GTCTTC GAAGAC(2/6)
1054 GTCTTC
BpmI 457 CTCCAG CTGGAG(16/14)
334 CTGGAG
BpuAI 1033 GTCTTC GAAGAC(2/6)
1054 GTCTTC
BsaI 55 GGTCTC GGTCTC(1/5)
528 GGTCTC
BsmAI 56 GTCTC GTCTC(1/5)
529 GTCTC
BsrFI 494 GCCGGC [GA]^CCGG[CT]
995 ACCGGT
CfoI 297 GCGC GCG^C
360 GCGC
Cfr10I 494 GCCGGC [GA]^CCGG[CT]
995 ACCGGT
EaeI 290 TGGCCA [CT]^GGCC[GA]
492 TGGCCG
EcoT14I 476 CCAAGG C^C[AT][AT]GG
719 CCAAGG
HhaI 297 GCGC GCG^C
360 GCGC
HinP1I 297 GCGC G^CGC
360 GCGC
HphI 281 TCACC GGTGA(8/7)
986 TCACC
MaeII 920 ACGT A^CGT
941 ACGT
MaeIII 133 GTGAC ^GT[ACGT]AC
985 GTCAC
PshAI 66 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
135 GACCCTCGTC
PvuII 763 CAGCTG CAG^CTG
934 CAGCTG
StyI 476 CCAAGG C^C[AT][AT]GG
719 CCAAGG
TfiI 230 GAATC G^A[AT]TC
787 GATTC
Tsp45I 133 GTGAC ^GT[GC]AC
985 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI Sau3AI Sau96I ScrFI
SfcI TaqI TseI Tsp509I TspRI
TthHB8I XcmI