Restriction map for
pF1KB8250
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# NO CUT ENZYMES ( 191 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaI KasI KpnI Ksp632I
KspI MaeII MaeIII MamI MfeI
MluI MluNI MroI MscI MslI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI SwaI TaqI
TfiI TseI Tsp45I TspRI Tth111I
TthHB8I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AciI 5 CCGC CCGC(-3/-1)
AflIII 176 ACATGT A^C[GA][CT]GT
AlwI 15 GGATC GGATC(4/5)
ApaI 359 GGGCCC GGGCC^C
BanII 359 GGGCCC G[GA]GC[CT]^C
BbvCI 322 GCTGAGG CCTCAGC(-5/-2)
BcnI 336 CCGGG CC^[GC]GG
BmyI 359 GGGCCC G[AGT]GC[ACT]^C
BpmI 301 CTGGAG CTGGAG(16/14)
BsmAI 169 GAGAC GTCTC(1/5)
Bsp1286I 359 GGGCCC G[AGT]GC[ACT]^C
BspHI 152 TCATGA T^CATGA
BspLU11I 176 ACATGT A^CATGT
BstF5I 333 CATCC GGATG(2/0)
BstNI 300 CCTGG CC^[AT]GG
BstYI 15 GGATCT [GA]^GATC[CT]
DdeI 323 CTGAG C^T[ACGT]AG
DraII 358 AGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 11 CCGTGG C^C[GA][CT]GG
EaeI 2 TGGCCG [CT]^GGCC[GA]
EcoO109I 358 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRII 300 CCTGG ^CC[AT]GG
EcoT14I 215 CCAAGG C^C[AT][AT]GG
Fnu4HI 4 GCCGC GC^[ACGT]GC
FokI 333 CATCC GGATG(9/13)
HapII 336 CCGG C^CGG
HpaII 336 CCGG C^CGG
HphI 78 GGTGA GGTGA(8/7)
ItaI 4 GCCGC GC^[ACGT]GC
MflI 15 GGATCT [GA]^GATC[CT]
MnlI 325 GAGG CCTC(7/6)
MspA1I 235 CAGCTG C[AC]G^C[GT]G
MspI 336 CCGG C^CGG
MvaI 300 CCTGG CC^[AT]GG
MwoI 352 GCACGAAGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 336 CCGGG CC^[GC]GG
NlaIV 359 GGGCCC GG[ACGT]^[ACGT]CC
PvuII 235 CAGCTG CAG^CTG
RcaI 152 TCATGA T^CATGA
SfaNI 342 GATGC GCATC(5/9)
SphI 126 GCATGC GCATG^C
StuI 32 AGGCCT AGG^CCT
StyI 215 CCAAGG C^C[AT][AT]GG
XbaI 18 TCTAGA T^CTAGA
XhoII 15 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
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Asp700I 30 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
220 GAAGCAATTC
BfaI 19 CTAG C^TAG
280 CTAG
BsaJI 11 CCGTGG C^C[ACGT][ACGT]GG
215 CCAAGG
BssKI 300 CCTGG ^CC[ACGT]GG
336 CCGGG
Cac8I 84 GCTCGC GC[ACGT]^[ACGT]GC
126 GCATGC
Cfr13I 359 GGGCC G^G[ACGT]CC
360 GGCCC
DpnII 16 GATC ^GATC
150 GATC
MaeI 19 CTAG C^TAG
280 CTAG
MboI 16 GATC ^GATC
150 GATC
MseI 272 TTAA T^TAA
314 TTAA
NdeII 16 GATC ^GATC
150 GATC
NspI 126 GCATGC [GA]CATG^[CT]
176 ACATGT
Sau3AI 16 GATC ^GATC
150 GATC
Sau96I 359 GGGCC G^G[ACGT]CC
360 GGCCC
ScrFI 300 CCTGG CC^[ACGT]GG
336 CCGGG
Tru9I 272 TTAA T^TAA
314 TTAA
Tsp509I 225 AATT ^AATT
270 AATT
XmnI 30 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
220 GAAGCAATTC
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# 3 OR MORE CUT ENZYMES ( 4 enzymes) #
#########################################
AluI HaeIII MboII NlaIII