Restriction map for
pF1KB8249
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRV EcoT22I
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MunI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI Sau96I SbfI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 26 CGCG CG^CG
AcsI 372 GAATTC [GA]^AATT[CT]
Alw44I 589 GTGCAC G^TGCAC
AlwNI 503 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 589 GTGCAC G^TGCAC
ApoI 372 GAATTC [GA]^AATT[CT]
BanII 643 GAGCTC G[GA]GC[CT]^C
BclI 404 TGATCA T^GATCA
BcnI 602 CCCGG CC^[GC]GG
BsaJI 121 CCATGG C^C[ACGT][ACGT]GG
BsrBI 676 GAGCGG CCGCTC(-3/-3)
BsrI 520 ACTGG ACTGG(1/-1)
BstAPI 502 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 470 GGATG GGATG(2/0)
BstNI 548 CCAGG CC^[AT]GG
BstUI 26 CGCG CG^CG
DsaI 121 CCATGG C^C[GA][CT]GG
EcoNI 705 CCTGCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 372 GAATTC G^AATTC
EcoRII 548 CCAGG ^CC[AT]GG
EcoT14I 121 CCATGG C^C[AT][AT]GG
FauI 678 GCGGG CCCGC(4/6)
FbaI 404 TGATCA T^GATCA
FokI 470 GGATG GGATG(9/13)
HapII 603 CCGG C^CGG
HpaII 603 CCGG C^CGG
MslI 465 CACTTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 562 CAGCTG C[AC]G^C[GT]G
MspI 603 CCGG C^CGG
MvaI 548 CCAGG CC^[AT]GG
MvnI 26 CGCG CG^CG
NciI 602 CCCGG CC^[GC]GG
NcoI 121 CCATGG C^CATGG
NdeI 278 CATATG CA^TATG
NlaIII 122 CATG CATG^
PleI 685 GAGTC GAGTC(4/5)
PvuII 562 CAGCTG CAG^CTG
SacI 643 GAGCTC GAGCT^C
SapI 640 GAAGAGC GCTCTTC(1/4)
ScaI 446 AGTACT AGT^ACT
StyI 121 CCATGG C^C[AT][AT]GG
TfiI 552 GAATC G^A[AT]TC
Tsp45I 136 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AfaI 51 GTAC GT^AC
447 GTAC
AspHI 589 GTGCAC G[AT]GC[AT]^C
643 GAGCTC
BmyI 589 GTGCAC G[AGT]GC[ACT]^C
643 GAGCTC
BsiHKAI 589 GTGCAC G[AT]GC[AT]^C
643 GAGCTC
BsiYI 159 CCTGTGTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
705 CCTGCGTGAGG
BslI 159 CCTGTGTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
705 CCTGCGTGAGG
Bsp1286I 589 GTGCAC G[AGT]GC[ACT]^C
643 GAGCTC
BssKI 548 CCAGG ^CC[ACGT]GG
602 CCCGG
Cac8I 621 GCTGGC GC[ACGT]^[ACGT]GC
678 GCGGGC
CfoI 27 GCGC GCG^C
524 GCGC
DpnII 405 GATC ^GATC
529 GATC
HaeIII 534 GGCC GG^CC
699 GGCC
HhaI 27 GCGC GCG^C
524 GCGC
HinP1I 27 GCGC G^CGC
524 GCGC
HinfI 552 GAATC G^A[ACGT]TC
685 GAGTC
HphI 402 GGTGA GGTGA(8/7)
567 GGTGA
MboI 405 GATC ^GATC
529 GATC
MseI 89 TTAA T^TAA
328 TTAA
NdeII 405 GATC ^GATC
529 GATC
NlaIV 168 GGTTCC GG[ACGT]^[ACGT]CC
412 GGAGCC
PstI 476 CTGCAG CTGCA^G
574 CTGCAG
RsaI 51 GTAC GT^AC
447 GTAC
Sau3AI 405 GATC ^GATC
529 GATC
ScrFI 548 CCAGG CC^[ACGT]GG
602 CCCGG
SfcI 476 CTGCAG C^T[GA][CT]AG
574 CTGCAG
StuI 533 AGGCCT AGG^CCT
698 AGGCCT
Tru9I 89 TTAA T^TAA
328 TTAA
Tsp509I 110 AATT ^AATT
373 AATT
TspRI 58 CAGTG CA[GC]TG[ACGT][ACGT]^
230 CAGTG
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
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AciI AluI BbvI BseRI BspMI
DdeI EarI Fnu4HI ItaI Ksp632I
MaeIII MboII MnlI MwoI TseI