Restriction map for
pF1KB8248
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BanI
BbeI BbvCI BcgI BciVI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BsgI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BsrI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DraIII Eam1105I Eco47III EcoNI EcoO65I
EcoRI EcoRV EcoT22I FspI HaeII
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MspA1I MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccII 116 CGCG CG^CG
AcyI 22 GACGCC G[GA]^CG[CT]C
AfaI 174 GTAC GT^AC
AocI 237 CCTGAGG CC^T[ACGT]AGG
AspHI 613 GAGCTC G[AT]GC[AT]^C
BamHI 501 GGATCC G^GATCC
BbrPI 300 CACGTG CAC^GTG
BbsI 658 GTCTTC GAAGAC(2/6)
BclI 290 TGATCA T^GATCA
BglI 409 GCCTGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 616 CTCCAG CTGGAG(16/14)
BpuAI 658 GTCTTC GAAGAC(2/6)
BsaAI 300 CACGTG [CT]AC^GT[GA]
BsaHI 22 GACGCC G[GA]^CG[CT]C
BsiEI 182 CGGCCG CG[GA][CT]^CG
BsiHKAI 613 GAGCTC G[AT]GC[AT]^C
BsmAI 10 GAGAC GTCTC(1/5)
BsmBI 10 GAGACG CGTCTC(1/5)
BsmFI 487 GGGAC GGGAC(10/14)
BsmI 402 GAATGC GAATGC(1/-1)
BsrBI 283 GAGCGG CCGCTC(-3/-3)
BssSI 493 CTCGTG CACGAG(-5/-1)
BstUI 116 CGCG CG^CG
BstXI 344 CCATCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 501 GGATCC [GA]^GATC[CT]
Bsu36I 237 CCTGAGG CC^T[ACGT]AGG
CfoI 470 GCGC GCG^C
DrdI 151 GACACCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 350 CCGTGG C^C[GA][CT]GG
EagI 182 CGGCCG C^GGCCG
EclXI 182 CGGCCG C^GGCCG
Eco52I 182 CGGCCG C^GGCCG
Eco81I 237 CCTGAGG CC^T[ACGT]AGG
EcoT14I 590 CCAAGG C^C[AT][AT]GG
FbaI 290 TGATCA T^GATCA
FseI 179 GGCCGGCC GGCCGG^CC
HhaI 470 GCGC GCG^C
Hin1I 22 GACGCC G[GA]^CG[CT]C
HinP1I 470 GCGC G^CGC
MflI 501 GGATCC [GA]^GATC[CT]
MvnI 116 CGCG CG^CG
PleI 478 GACTC GAGTC(4/5)
PmaCI 300 CACGTG CAC^GTG
PmlI 300 CACGTG CAC^GTG
RsaI 174 GTAC GT^AC
SacI 613 GAGCTC GAGCT^C
SapI 273 GAAGAGC GCTCTTC(1/4)
SmaI 530 CCCGGG CCC^GGG
StyI 590 CCAAGG C^C[AT][AT]GG
TaqI 227 TCGA T^CGA
TfiI 676 GAATC G^A[AT]TC
Tsp45I 149 GTGAC ^GT[GC]AC
TthHB8I 227 TCGA T^CGA
XhoII 501 GGATCC [GA]^GATC[CT]
XmaCI 530 CCCGGG C^CCGGG
XmaI 530 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 299 ACACGT A^C[GA][CT]GT
654 ACACGT
AluI 572 AGCT AG^CT
614 AGCT
AvaI 62 CTCGGG C^[CT]CG[GA]G
530 CCCGGG
BseRI 4 GAGGAG GAGGAG(10/8)
7 GAGGAG
BsoBI 62 CTCGGG C^[CT]CG[GA]G
530 CCCGGG
BsrFI 180 GCCGGC [GA]^CCGG[CT]
376 GCCGGC
BstF5I 509 CATCC GGATG(2/0)
426 GGATG
Cfr10I 180 GCCGGC [GA]^CCGG[CT]
376 GCCGGC
DraII 66 GGGTCCT [GA]G^G[ACGT]CC[CT]
487 GGGACCC
EaeI 118 CGGCCA [CT]^GGCC[GA]
182 CGGCCG
EarI 451 CTCTTC CTCTTC(1/4)
273 GAAGAG
EcoO109I 66 GGGTCCT [GA]G^G[ACGT]CC[CT]
487 GGGACCC
FokI 509 CATCC GGATG(9/13)
426 GGATG
HgaI 12 GACGC GACGC(5/10)
22 GACGC
HinfI 478 GACTC G^A[ACGT]TC
676 GAATC
Ksp632I 451 CTCTTC CTCTTC(1/4)
273 GAAGAG
MaeIII 149 GTGAC ^GT[ACGT]AC
692 GTTAC
MslI 345 CATCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
348 CACCGTGGTG
NaeI 180 GCCGGC GCC^GGC
376 GCCGGC
NgoMIV 180 GCCGGC G^CCGGC
376 GCCGGC
NlaIII 414 CATG CATG^
441 CATG
PpuMI 66 GGGTCCT [GA]G^G[AT]CC[CT]
487 GGGACCC
SfaNI 373 GATGC GCATC(5/9)
427 GATGC
TspRI 609 CACTG CA[GC]TG[ACGT][ACGT]^
622 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AlwI AlwNI AvaII BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI Bsp1286I BspMI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
FauI Fnu4HI HaeIII HapII HpaII
HphI ItaI MaeII MboI MboII
MnlI MspI MvaI MwoI NciI
NdeII NlaIV PstI Sau3AI Sau96I
ScrFI SfcI TseI