Restriction map for
pF1KB8242
##################################
# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaII AviII AvrII
BalI BbrPI BbvCI BcgI BclI
BfaI BfrI BglI BglII BlnI
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
ClaI CpoI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I KspI MaeI MaeII
MamI MluI MluNI MroI MscI
MslI MvnI NcoI NheI NotI
NruI NsiI NspI PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tsp45I Van91I XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 358 GTAGAC GT^[AC][GT]AC
AcyI 22 GGCGCC G[GA]^CG[CT]C
AspI 174 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 9 CCCGGG C^[CT]CG[GA]G
BamHI 96 GGATCC G^GATCC
BanI 22 GGCGCC G^G[CT][GA]CC
BbeI 22 GGCGCC GGCGC^C
BbsI 521 GAAGAC GAAGAC(2/6)
BlpI 535 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 535 GCTCAGC GC^T[ACGT]AGC
BpuAI 521 GAAGAC GAAGAC(2/6)
BsaHI 22 GGCGCC G[GA]^CG[CT]C
BsmFI 410 GTCCC GGGAC(10/14)
BsoBI 9 CCCGGG C^[CT]CG[GA]G
BspHI 584 TCATGA T^CATGA
BsrDI 186 CATTGC GCAATG(2/0)
BstBI 518 TTCGAA TT^CGAA
BstYI 96 GGATCC [GA]^GATC[CT]
CelII 535 GCTCAGC GC^T[ACGT]AGC
CfoI 23 GCGC GCG^C
DraI 283 TTTAAA TTT^AAA
EcoRI 423 GAATTC G^AATTC
HaeII 22 GGCGCC [GA]GCGC^[CT]
HgaI 488 GCGTC GACGC(5/10)
HhaI 23 GCGC GCG^C
Hin1I 22 GGCGCC G[GA]^CG[CT]C
HinP1I 23 GCGC G^CGC
HinfI 408 GAGTC G^A[ACGT]TC
HphI 461 TCACC GGTGA(8/7)
KasI 22 GGCGCC G^GCGCC
MaeIII 110 GTTAC ^GT[ACGT]AC
MboII 521 GAAGA GAAGA(8/7)
MfeI 428 CAATTG C^AATTG
MflI 96 GGATCC [GA]^GATC[CT]
MspA1I 100 CCGCTG C[AC]G^C[GT]G
MunI 428 CAATTG C^AATTG
NarI 22 GGCGCC GG^CGCC
NdeI 560 CATATG CA^TATG
NlaIII 585 CATG CATG^
NspV 518 TTCGAA TT^CGAA
PflFI 174 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 408 GAGTC GAGTC(4/5)
RcaI 584 TCATGA T^CATGA
SfcI 122 CTGTAG C^T[GA][CT]AG
SfoI 22 GGCGCC GGC^GCC
SfuI 518 TTCGAA TT^CGAA
SgrAI 24 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 9 CCCGGG CCC^GGG
TspRI 339 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 174 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 96 GGATCC [GA]^GATC[CT]
XmaCI 9 CCCGGG C^CCGGG
XmaI 9 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AluI 126 AGCT AG^CT
514 AGCT
AlwI 97 GATCC GGATC(4/5)
96 GGATC
BanII 59 GGGCTC G[GA]GC[CT]^C
500 GGGCTC
BbvI 118 GCTGC GCAGC(8/12)
485 GCTGC
BciVI 196 GGATAC GTATCC(6/5)
528 GGATAC
BmyI 59 GGGCTC G[AGT]GC[ACT]^C
500 GGGCTC
BsmAI 217 GAGAC GTCTC(1/5)
315 GAGAC
Bsp1286I 59 GGGCTC G[AGT]GC[ACT]^C
500 GGGCTC
BsrFI 25 GCCGGC [GA]^CCGG[CT]
224 GCCGGC
BstF5I 16 CATCC GGATG(2/0)
135 GGATG
Cfr10I 25 GCCGGC [GA]^CCGG[CT]
224 GCCGGC
Cfr13I 70 GGGCC G^G[ACGT]CC
381 GGCCC
DpnII 97 GATC ^GATC
459 GATC
DraII 69 AGGGCCT [GA]G^G[ACGT]CC[CT]
380 AGGCCCT
EcoO109I 69 AGGGCCT [GA]G^G[ACGT]CC[CT]
380 AGGCCCT
FauI 32 GCGGG CCCGC(4/6)
47 GCGGG
FokI 16 CATCC GGATG(9/13)
135 GGATG
HaeIII 71 GGCC GG^CC
381 GGCC
MboI 97 GATC ^GATC
459 GATC
MseI 209 TTAA T^TAA
284 TTAA
MwoI 23 GCGCCGGCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
118 GCTGCTGTAGC
NaeI 25 GCCGGC GCC^GGC
224 GCCGGC
NdeII 97 GATC ^GATC
459 GATC
NgoMIV 25 GCCGGC G^CCGGC
224 GCCGGC
Sau3AI 97 GATC ^GATC
459 GATC
Sau96I 70 GGGCC G^G[ACGT]CC
381 GGCCC
SfaNI 148 GATGC GCATC(5/9)
15 GCATC
TaqI 467 TCGA T^CGA
519 TCGA
Tru9I 209 TTAA T^TAA
284 TTAA
TseI 118 GCTGC G^C[AT]GC
485 GCTGC
TthHB8I 467 TCGA T^CGA
519 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AcsI AfaI ApoI BcnI
BpmI BsaJI BsiYI BslI BssKI
BstNI Cac8I DdeI EcoRII Fnu4HI
HapII HpaII ItaI MnlI MspI
MvaI NciI NlaIV RsaI ScrFI
Tsp509I